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Salinity and drought are important agro-environmental problems occurring separately as well as together with the combined occurrence increasing with time due to climate change. Screening of bread wheat genotypes against salinity or drought alone is common; however, little information is available on the response of wheat genotypes to a combination of these stresses. This study investigates the response of a salt-resistant (SARC-1) and a salt-sensitive (7-Cerros) wheat genotype to drought at different growth stages under non-saline (ECe 2.1 dS m-1) and saline soil (ECe 15 dS m-1) conditions. Drought was applied by withholding water for 21 days at a particular growth stage viz. tillering, booting, and grain filling stages. At booting stage measurements regarding water relations, leaf ionic composition and photosynthetic attributes were made. At maturity grain yield and different yield, components were recorded. Salinity and drought significantly decreased grain yield and different yield components with a higher decrease in the case of combined stress of salinity 9 drought. The complete drought treatment (drought at tillering + booting + grain filling stages) was most harmful for wheat followed by drought at booting stage and grain filling–tillering stages, respectively. The salt-resistant wheat genotype SARC-1 performed better than the saltsensitive genotype 7-Cerros in different stress treatments. A decrease in the water and turgor potentials, photosynthetic and transpiration rates, stomatal conductance, leaf K+, and increased leaf Na+ were the apparent causes of growth and yield reduction of bread wheat due to salinity, drought, and salinity 9 drought.
Four tobacco lines over-expressing LlaNAC (FJ423495) gene have been assessed for various parameters like abiotic stress response, phytochemical constituents, biomass composition, response to elevated carbon dioxide (CO₂), etc. All the analyses have been carried out in T2 generation of the transgenic plants with proven genetic stability. In response to the heat and drought stress, the accumulation of antioxidant enzymes elevated up to 2.5-fold. Photosynthetic pigments, i.e., Chl a, Chl b and xanthophylls, and phenolics in the transgenic lines were found significantly (two–fourfold) enhanced in comparison to wild-type (WT) plants. The transgenic lines were also shown capturing 5–10 times more CO₂ from the environment, which presumably gets accumulated as biomass. However, ratio of dry weight to fresh weight was slightly in favor of the WTs. Cellulose, hemi-cellulose and lignin contents in stem and leaves of each of the transgenic lines varied, and overlapped with the lignocellulosic contents of the WT plants. In conclusion, LlaNAC gene shows promise for exploitation in design of the future crops with various end uses.
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