Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 11

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Phylogenetic diversity of bacteria and Archaea associated with the marine sponge Pachychalina sp.

100%
Cheng N., Fang Z., Huang H., Fang Z., Wu X., Bao S.
Polish Journal of Ecology
|
2008
|
tom 56
|
nr 3
505-510
Molecular techniques were employed to document the microbial diversity associated with the marine sponge Pachychalina sp. from South China Sea in March 2003. Using the total microbial DNA as template, bacterial and archaeal 16S rDNAs were amplified by PCR with universal primers. Amplified products were cloned, sequenced and secondarily amplified by PCR. Then the secondarily amplified products were purified to be further characterized by amplified rDNA restriction analysis (ARDRA). According to the enzyme restriction mapping, the apparent difference among them were disclosed. 22 bacterial cloned partial sequences were acquired and most of them were related to proteobacterium. Also, 7 archaeal cloned partial sequences were acquired and a phylogenetic tree was built up.Result shows the prolific bacterial and archaeal diversity of marine sponge Pachychalina sp.
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Optimization of microwave-assisted extraction of flavonoids from young barley leaves

94%
Gao T., Min Z., Fang Z., Zhong Q.
International Agrophysics
|
2017
|
tom 31
|
nr 1
A central composite design combined with response surface methodology was utilized to optimise microwave-assisted extraction of flavonoids from young barley leaves. The results showed that using water as solvent, the optimum conditions of microwave-assisted extraction were extracted twice at 1.27 W g-1 microwave power and liquid-solid ratio 34.02 ml g-1 for 11.12 min. The maximum extraction yield of flavonoids (rutin equivalents) was 80.78±0.52%. Compared with conventional extraction method, the microwave-assisted extraction was more efficient as the extraction time was only 6.18% of conventional extraction, but the extraction yield of flavonoids was increased by 5.47%. The main flavonoid components from the young barley leaf extract were probably 33.36% of isoorientin-7-O-glueoside and 54.17% of isovitexin-7-O-glucoside, based on the HPLC-MS analysis. The barley leaf extract exhibited strong reducing power as well as the DPPH radical scavenging capacity.
3

Selection of reference genes for quantitative real-time PCR during flower bud development in CMS7311 of heading Chinese cabbage (Brassica rapa L. ssp. pekinensis)

83%
Xu X., Yang Z., Sun X., Zhang L., Fang Z.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 03
The accuracy of quantitative real-time PCR (qRT-PCR) depends on the stability of the reference gene used for normalization. In heading Chinese cabbage (Brassica rapa L. ssp. pekinensis), the most stable reference genes for qRT-PCR during flower bud development have not been elucidated. In this study, the statistical software geNorm was used to test eight candidate reference genes during flower bud development in male sterile (Ms) and fertile (Mf) plants. The result revealed that the stability order was Tub/GAPDH > Cyp > EF1a > U34559 > BrTip41 > Apr > 18S rRNA, Tub and GAPDH were the most stable genes [average expression stability (M) 0.614], and the combined use of six reference genes [pairwise variation (V) 0.15] was suggested to be the optimal reference gene for qRT-PCR during flower bud development. Furthermore, the expressions of BcPME31 during flower bud development normalized with the combined use of six reference genes and with GAPDH or Tub alone were compared; the various results also suggested that selection of the optimal reference gene was necessary for gene expression analysis.
4

Content and species of extracellular phosphorus in activated sludge of biological phosphorus removal systems

83%
Long X., Tang R., Xie C., Fang Z., Li Y.
Polish Journal of Environmental Studies
|
2019
|
tom 28
|
nr 5
Extracellular polymeric substances (EPS) contain a large amount of phosphorus, but the content of extracellular phosphorus in activated sludge and the decomposition of extracellular polyphosphate (polyP) in extraction process is still unclear. In this study, the extraction efficiencies of extracellular phosphorus using sonication, cation exchange resin (CER) and sonication-CER methods were investigated and compared, taking the aerobic sludge from four lab-scale A/O-SBR reactors at different temperatures and in different matrices as the objects. The sonication-CER method was an efficient and reliable method for extracting EPS and extracellular phosphorus, which could avoid the decomposition of extracellular polyP and the massive lysis of a bacterial cell. Importantly, utilizing CER with two successive alkaline washes was a key factor for efficiently extracting extracellular phosphorus. The extracellular phosphorus content of aerobic-activated sludge in 4 A/O-SBR reactors was 28.17~73.13 mg P/g VSS, accounting for 59.6~74.1% TPsludge. Thereby phosphorus of sludge floc was mainly located in EPS. Furthermore, polyP was the main species of extracellular phosphorus in EBPR sludge, which mainly existed in the inner layer of sludge floc. There was a close relationship between the content and species of extracellular phosphorus and the EBPR performance of activated sludge.
5

Improved gene targeting frequency in marine-derived filamentous fungus Aspergillus glaucus by disrupting ligD

72%
Fang Z., Zhang Y., Cai M., Zhang J., Zhang Y., Zhou X.
Journal of Applied Genetics
|
2012
|
tom 53
|
nr 3
Filamentous fungi from the marine environment have shown great potential as cell factories for the production of pharmacologically active metabolites, but extremely low frequency of homologous recombination brings difficulty to further molecular biology studies. To bypass this problem and develop a highly efficient gene targeting system in marine-derived filamentous fungus Aspergillus glaucus, LigD, a homolog of Neurospora crassa Mus-53 which is considered to play a significant role in nonhomologous end joining (NHEJ), was coloned and deleted, and frequency of targeted gene replacement (TGR) increased dramatically from <2 % to 85 % in comparison with that in the wild type, when containing 1000 bp of homologous flanking sequence. Such results strongly indicate that AgLigD is indeed involved in the repair of NHEJ in A. glaucus and functions in this pathway. Furthermore, the AgLigD-defective mutant has no discernible differences with wild type regarding sensitivity to mutagens and UV, growth characteristics and transformation frequency. The AgligD-deficient transformant, as the first NHEJ-defective mutant in the field of marine-derived filamentous fungus, will help in expediting studies of molecular biology of marine-derived microorganisms.
6

Identification of candidate genes associated with male sterility in CMS7311 of heading Chinese cabbage (Brassica campestris L. ssp. pekinensis)

72%
Xu X., Sun X., Zhang J., Huang W., Zhang L., Fang Z.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 11
Polima (pol) cytoplasmic male sterility (CMS) is reported to be regulated by orf224, but the molecular mechanisms of CMS are less known. In previous research, CMS7311 of heading Chinese cabbage (Brassica campestris L.ssp. pekinensis) was successfully generated from a pol-like CMS, named 77A. To further understand the molecular mechanisms of this male sterility, cDNA-AFLP and qRT-PCR were used to identify genes differentially expressed during flower bud development between cytoplasmic male-sterile plant (Ms) and male-fertile plant (Mf) derived from the hybrid of CMS7311 and a restorer (Rf). Thirty-two transcripts of different fragments (TDFs) of over 80 bp in length were identified using cDNA-AFLP. Among them, ten TDFs were found expressing in Ms bud, and the others expressing in Mf bud. Gene ontology analysis revealed that these genes were involved in stress response, synthesis and metabolism of fatty acid, cell reconstruction, etc. Further, five genes closely related to male sterile were selected and their expression during the flower bud development of Ms and Mf line were investigated by qRT-PCR. Three genes, BcGRP17, BcMS2, and BcPME31, which were necessary for normal male organ development, showed significantly lower expressions in Ms bud; two genes, BcROPGEF8 and BcTNL3, were induced with significantly higher expression in Ms bud by unknown stress and involved in the formation of male sterility. Further work will be focused on cloning these genes and identifying their functions in the male-sterile process of heading Chinese cabbage CMS7311.
7

Biological nitrogen removal in a flow-separating biochemical reactor with coral sand

72%
Long X., Tang R., Zhan B., Fang Z., Xie C., Li Y.
Polish Journal of Environmental Studies
|
2019
|
tom 28
|
nr 5
In order to provide some support for economically and effectively resolving the problem of water pollution around the islands in the South China Sea, biological nitrogen removal (BNR) performance and biotechnological characterization of the FSBR filled with abandoned coral sand were investigated. The results showed that the TN removal efficiency of the FSBR with a good performance of simultaneous nitrification and denitrification (SND) was 74.68±6.49% during the stable operation. The total biomass and removal efficiency of pollutants in the three reaction regions all decreased along the flow direction. Therefore, the first region was the key area for SND reaction and TN removal. In the first region, the nitrification process was the result of the combination of autotrophic nitrification and heterotrophic nitrification. Meanwhile, aerobic denitrification played a key role in the process of denitrification. In the region, the most denitrifying bacteria belonged to Proteobacteria, in which unclassified_ f _Comamonadace, Acidovorax and Dokdonella were the major aerobic denitrifying bacteria.
8

A blastocyst biopsy approach for preimplantation genetic diagnosis technique that affects the expression of SNAP-α in mice

60%
Zhang Y., Fang Z., Lu H., Li Y., Baloch Z., Liu Y., Yang S., Lu Z., Ding C.
Reproductive Biology
|
2020
|
tom 20
|
nr 3
9

Antioxidant and anti-diabetic effects of Auricularia auricular polysaccharides and their degradation by artificial gastrointestinal digestion - Bioactivity of Auricularia auricular polysaccharides and their hydrolysates

60%
Lu A., Yu M., Shen M., Fang Z., Xu Y., Wang S., Zhang Y., Wang
Acta Scientiarum Polonorum. Technologia Alimentaria
|
2018
|
tom 17
|
nr 3
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Seasonal variations in the abundance and sinking flux of biogenic silica in Daya Bay, Northern South China Sea

60%
Zhao X., Yang W., Ma H., Li J., Chen M., Fang Z., Zhang X., Zeng J., Qiu Y., Zheng M.
Oceanologia
|
2019
|
tom 61
|
nr 2
11

Identification of seed-specific transcription factors from a global analysis of gene activity during the Arabidopsis life cycle

49%
Le B. H., Bui A. Q., Pelletier J., Kwong L., Fang Z., Horvath S., Drews G. N., Fischer R. L., Okamuro J. K., Harada J. J., Goldberg R. B.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2005
|
tom 47
|
nr 1
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.