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1

Nonlinear drift of the spring gravimeter caused by air pressure from the Kunming GS15 gravimeters

100%
Wei J., Shen W., Li H., Liu Z.
Polish Maritime Research
|
2016
|
tom 23
|
nr Special Issue S1
In order to monitor and correct the meteorological factors of the spring gravity meter, the characteristics of the time varying gravity changes caused by meteorological factors were analyzed. Kunming GS15 gravity meter from 2007 continuous gravity observation has been carried out with the sampling rate of the pressure observation. In this study, we first compare the effects of 4 types of gravity meter and 3 different types of stations on the gravity observed seasonal gravity signals. It is indicated that the observed seasonal gravity signal of the cave is only 1/10, and there is a constant temperature and constant pressure device. Compared with the same time, the gravity signal of the gravity signal is about 100 times smaller. The influence of the pressure load of the gravity meter is tested by using the theory of pressure. The results show that only the actual value of 2cpd - 3cpd pressure load varies from -0.395 to -0.280×10-8ms-2 , and the 1cpd to 1 cpm periodic partial type gravity meter is also in accordance with the law of gravity and air pressure. And with the characteristics of time lag. In this paper, the nonlinear zero drift parameters of the linear regression model with time lag and the time series of the GS15 gravity meter are used to simulate the nonlinear zero drift parameters of the gravity meter. The results show that the gravity signal contains time lag 35 hours, and the air pressure admittance is 0.8 × 10-8ms-2/mbar. The correlation can reach 79%. The gravity changes signal and satellite gravity as well as the gravity water load signal of the land water model are the same as the gravity water load signal in the autumn as the minimum value, and the seasonal variation of the maximum value of gravity in summer
2

Gallbladder interstitial Cajal-like cells and gallbladder contractility in patients with cholelithiasis: a prospective study

100%
Ding R., Wei J., Xu J.
Folia Histochemica et Cytobiologica
|
2019
|
tom 57
|
nr 2
3

Rice florigen gene Hd3a has conserved functions in callus development

88%
Wu Y., Wei J., Choi S.-C., Fei Y., Xiong F.
Acta Physiologiae Plantarum
|
2019
|
tom 41
|
nr 07
The transition of meristems is an important developmental process for crop plants. Florigen is considered to be produced in leaves, then moves into the shoot apical meristem (SAM), triggers the transition from the vegetative to the reproductive phase. However, little is known whether Florigen functions in callus development or not. By fused reporter gene β-glucuronidase (GUS) to 1.7 kb promoter of Heading date 3a (Hd3a), GUS signals were detected in the scutellum cells, as well as in green point of the putative transgenic calli. Quantitative RT-PCR results demonstrated that the expression level of Hd3a was increased gradually over time along with the transition from scutellum-deprived callus to shoot. As reported that ectopic expression of FT-like genes caused earlier flowering, we also found that 80% constitutive expression of Hd3a transgenic callus showed formation floral-like organ structures. However, Hd3a RNA interference (RNAi) transgenic calli did not show any obvious phenotype, although AP1 or AP1-like genes—OsMADS14, OsMADS15, and OsMADS18- expression level is decreased during callus development. Both in Hd3a and RFT1 overexpression transgenic calli, Hd3a also modulated AP1 or AP1-like genes, as well AEPALLATA (SEP)-like gene, OsMADS34 during green point formation. Meanwhile, transgenic calli of RFT1and OsMADS50, but not OsEhd1, shared similar results as Hd3a. All of these findings suggested that florigen genes Hd3a and RFT1 have partial conserved functions in the transition of meristems during callus development.
4

DNA microarray gene expression profile of Mycobacterium tuberculosis when exposed to osthole

76%
Wei J., Guo N., Liang J., Yuan P., Shi Q., Tang X., Yu L.
Polish Journal of Microbiology
|
2013
|
tom 62
|
nr 1
Tuberculosis (TB), affecting one-third of the global population, kills an estimated two to three million people every year. The development of drug resistance is becoming a serious threat to any attempt to control this disease, which underscores the need for new agents targeting Mycobacterium tuberculosis (M. tuberculosis). Osthole (7-methoxy-8-isopentenoxycoumarin) is a coumarin derivative present in many medicinal plants. Previous studies have shown that osthole possesses antimycobacterial effects, however, the action mechanism of osthole is unclear. In the study, we used a commercial oligonucleotide microarray to determine the overall transcriptional response of M.tuberculosis H37Rv triggered by exposure to osthole. Analysis of the microarray data revealed that a total of 478 genes were differentially regulated by osthole. Of these, 241 genes were upregulated, and 237 genes were downregulated. Some of the important genes that were significantly regulated are related to different pathways such as fumarate reductase, class I peroxidase, cell wall, nitrate respiration, and protein synthesis. Real-time quantitative RT-PCR was performed for chosen genes to validate the microarray results. To our knowledge, this genome-wide transcriptomics approach has produced the first insights into the response of M. tuberculosis when exposed to osthole.
5

DNA methylation alterations of upland cotton (Gossypium hirsutum) in response to cold stress

76%
Fan H. H., Wei J., Li T. C., Li Z. P., Guo N., Cai Y. P., Lin Y.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 08
DNA methylation plays an important role in regulating gene expression in plants. In the experiment, we studied effects of cold on DNA methylation variation in upland cotton. Using the methylation-sensitive amplified polymorphism procedure, we chose 66 pairs of selective amplification primers to assess the status and levels of cytosine methylation. The hemimethylation of the external cytosine and the full methylation of the internal cytosine were scored. As a result, cold triggered the demethylation of hemimethylated or internally full methylated cytosine. With the prolongation of cold treatment, the demethylation loci increased and the methylation loci decreased. Nevertheless, this change could be reverted when cotton was subsequently recovered under normal temperature. In addition, 29 polymorphic bands that appeared in the electrophoretogram were sequenced. By homologous alignment analysis, most of these 29 fragments were identified as genes or DNA clones involved in abiotic stress response. The variation in methylation loci existed at both coding and non-coding regions. Furthermore, the expression of the abiotic stressrelated genes, GhCLSD (Seq21), GhARK (Seq22), GhARM (Seq15, Seq18, Seq19 and Seq21) and GhTPS (Seq8), were tested. The results revealed that cold treatment induced down-regulation of GhCLSD, GhARK and GhARM, but upregulated the expression of GhTPS. These changes were in accordance with the alteration of DNA methylation. Thus, cold may affect the gene expression via changing the methylation status in the cytosine nucleotide.
6

The accumulation of pigment in fiber related to proanthocyanidins synthesis for brown cotton

76%
Li T., Fan H., Li Z., Wei J., Lin Y., Cai Y.
Acta Physiologiae Plantarum
|
2012
|
tom 34
|
nr 2
Brown cotton is a kind of naturally colored cotton. Because of less processing and little dying, it is more friendlier to environment than white cotton. For brown cotton, pigment accumulation in fiber is one of the most important characteristics. In this study, we selected a brown fiber line and a white fiber cultivar to determine the factor that affects the pigmentation in brown fiber. Accordingly, fibers were collected to verify the presence of PAs by p-dimethylaminocinnamaldehyde (DMACA) and toluidine blue O (TBO) staining. The PAs content and related genes expressions were determined. As a result, there were obvious differences on the aspect of PAs synthesis in fiber between white cotton and brown cotton. For white fiber, the PAs content reached maximum at 5 DPA, and then gradually decreased to zero. But for brown fiber, the PAs content was increased from 5 to 15 DPA stage, and reached the maximum at the 15 DPA stage, then gradually decreased from 15 to 40 DPA stage. On the contrary, in white cotton, PAs were synthesized in the whole developmental stage from 5 to 40 DPA. And PAs content in brown fiber were far more than that in white fiber, which may be the reason why the brown pigment accumulated in brown fiber.
7
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Magnolol inhibits Streptococcus suis-induced inflammation and ROS formation via TLR2/MAPK/NF-κB signaling in RAW264.7 cells

64%
Zhang L., Wang J., Xu W., Sun Y., You J., Lu H., Song Y., Wei J., Li L.
Polish Journal of Veterinary Sciences
|
2018
|
tom 21
|
nr 1
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