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The effect of eight anthelmintics (Rintal, Fenbesan, Telmin, Banminth, Pyrantel, Nilverm, Levamisol and Bioscardina) on the α- and γ-amylases, trypsin, and lipase from pig's pancreas and gut of Ascaris suum was determined. In extracts from A. suum gut also the maltase, trehalase and saccharase activities were examined. All drugs tested did not influence on the host's α-amylase. Telmin and Bioscardina were inhibitors of γ-amylase, Rintal and Telmin - of trypsin, Fenbesan and Bioscardina - of lipase from pig's pancreas. Among the parasite's enzymes the lipase was the most sensitive. Its activity was decreased (35-60%) by Telmin, Nilverm and both pyrantel derivatives. The activity of maltase and trehalase was reduced by Levamisol and Banminth, that of saccharase by Levamisol. It is concluded that the anthelmintics Levamisol and Banminth seemed the most efficient among the tested drugs because they did not alter the activity of host's enzymes and, showed the inhibitory effect on three of parasite's enzymes.
Adult Ascaris suum were incubated at 37°C in Ringer solution for Ascaris (ARSb) and in ARSb with 0.2 % addition of glucose, maltose, trehalose, saccharose, starch and glycogen. Control worms were incubated in ARSb without natrium acetate (ARS0). The survival rate, motility and elasticity of each individual, changes of the body weight and glycogen contents in muscles were determined. The incubation in ARS0 was finished on the 7th day. The longest (11 days) lived the worms kept in the basic medium ARSb and ARSb with maltose. Regarding their influence on the survival rate of parasites, the rest sugars could be set in order as follows: glycogen and glucose (10 days), trehalose, saccharose and starch (9 days). In the media containing saccharose and starch worms showed reduced motility and elasticity. On the 7th day of incubation the glycogen level in muscles of control worms was reduced to 0.75% of its beginning concentration, whereas in muscles of Ascaris from media with glucose and trehalose over 50% and with maltose 43% of polysaccharide's reserves were preserved. A slight increase (10-15%) in average body weight of worms incubated with glucose, maltose and glycogen was observed, only in the control and medium with saccharose changes of worms' mass were significant, amounting to 30% and 80%, respectively.
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