Wyniki wyszukiwania

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Changes in the morphology of the acinar cells of the rat pancreas in the oedematous and necrotic types of experimental acute pancreatitis

100%

Krajewski E., Krajewski J., Spodnik J. H., Figarski A., Kubasik-Juraniec J.

Folia Morphologica

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2005

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tom 64

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nr 4

292-303

Limited experimental models of the oedematous and necrotic types of acute pancreatitis provide some understanding of the pathophysiology of this disease. Wistar rats were treated with cerulein at 10 mg/kg of body weight or with L-arginine at 1.5 or 3 g/kg of body weight in order to induce the oedematous or necrotic type of acute pancreatitis. After the induction period we examined samples of pancreata with light and electron microscopes. Morphological examination showed profound changes in the histology of the pancreas and its acinar cells and subcellular structures, especially in the group of rats which received a higher dose of L-arginine, amounting to 3 g/kg body weight. These included parenchymal haemorrhage and widespread acinar cell necrotic changes. 4-OH-TEMPO successfully prevented morphological deterioration as well as amylase release, suggesting that the severity of the two types of disease strongly depends on the intensity of the oxidative stress. Our results lend support to the assumption that reactive oxygen species play an axial role in the pathogenesis of both types of acute pancreatitis.

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Controlled cholesterol efflux from the aortic smooth muscle cells triggers microheterogeneity of plasma membrane lipids and induces modification of the mitochondrial topology

86%

Tukaj C., Syta-Ksiazek E., Zauszkiewicz A., Drozd K., Figarski A., Spodnik J. H., Wozniak M.

Folia Morphologica

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2009

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tom 68

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nr 4

244-246

It is generally accepted that phospholipids of plasma membrane display lateral segregation into small microdomains commonly known as lipid rafts. Such lateral lipid organization is under the control of cholesterol. Cholesterol depletion evolved by methyl-β-cyclodextrin (MCD) has been found to induce further marked perturbation in lateral lipid organization, evidenced in the high field part of electron paramagnetic resonance spectra of plasma membranes labelled with a spectroscopic probe, namely 5-doxyl-stearic acid (5DOXS). Such perturbation of surface lipid topo-logy has been found to induce distinct changes in the mitochondrial morpho-logy, i.e. switch from filamentous form into small granular form. (Folia Morphol 2009; 68, 4: 244–246)

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Catechol estrogen displays protective effect on albumin- important extracellular antioxidant being exposed to peroxyl radical generating system

86%

Figarski A., Walczak P., Szymendera J., Powierza T., Greci L., Wozniak M.

Acta Biochimica Polonica

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2003

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tom 50

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nr Supplement 1

4

4-OH-TEMPO prevents the morphological alteration of rat thymocytes primed to apoptosis by oxidative stress inducer ButOOH

58%

Kossowska E., Zauszkiewicz A., Kubasik-Juraniec J., Tukaj C., Spodnik J. H., Hallman A., Klimek J., Syta E., Figarski A., Wakabayashi T., Wozniak M.

Folia Morphologica

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2004

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tom 63

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nr 3

Thymocytes exposed to the pro-oxidant tert–butyl-hydroperoxide (ButOOH) display a number of dramatic changes in morphology similar to those observed in the case of dexamethasone-treated cells. Both reagents induce nuclear chromatin peripheral aggregation below the nuclear membrane. Some nuclei themselves break up producing two or more fragments. ButOOH-treated cells are morphologically characterised by cell shrinkage, extensive surface blebbing and, finally, fragmentation into membrane–bound apoptotic bodies composed of cytoplasm and tightly packed with or without nuclear fragments. An increased level of lipid hydroxyperoxides was detected after exposure of thymocytes to ButOOH. Both oxidative stress markers and morphological damage to cells were prevented by the antioxidant 4-OH-TEMPO.

Ograniczanie wyników

3 Folia Morphologica

1 Acta Biochimica Polonica

4 Figarski A.

3 Spodnik J. H.

3 Wozniak M.

2 Kubasik-Juraniec J.

2 Tukaj C.

2 Zauszkiewicz A.

1 Drozd K.

1 Greci L.

1 Hallman A.

1 Klimek J.

1 Kossowska E.

1 Krajewski E.

1 Krajewski J.

1 Powierza T.

1 Syta E.

1 Syta-Ksiazek E.

1 Szymendera J.

1 Wakabayashi T.

1 Walczak P.

1 2009

1 2005

1 2004

1 2003

Changes in the morphology of the acinar cells of the rat pancreas in the oedematous and necrotic types of experimental acute pancreatitis

Controlled cholesterol efflux from the aortic smooth muscle cells triggers microheterogeneity of plasma membrane lipids and induces modification of the mitochondrial topology

Catechol estrogen displays protective effect on albumin- important extracellular antioxidant being exposed to peroxyl radical generating system

4-OH-TEMPO prevents the morphological alteration of rat thymocytes primed to apoptosis by oxidative stress inducer ButOOH