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Bakterie najczęściej izolowane z klinicznych postaci mastitis u krów oraz ich wrażliwość na antybiotyki

100%

Lassa H., Kubiak J., Malkinska-Horodyska M.

Życie Weterynaryjne

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2013

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tom 88

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nr 08

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Phenotypical identyfication of atypical Staphylococcus aureus strains isolated from milk of cows from one herd

100%

Malinowski E., Lassa H., Tomaszewska J., Malkinska-Horodyska M.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 2

The aim of the study was to characterise the free coagulase-negative Staphylococcus sp. strains isolated from normal (9.4%) and inflamed quarter milk samples of dairy cows in one herd localised in southern Poland. The isolates were examined phenotypically by cultural features, tube coagulase test, clumping factor (CF), and commercial agglutination test. Total number of 427 strains were cultivated on different media: Congo red agar, DNase medium, mannitol salt agar, and crystal violet agar. About 25% of the strains were examined by the disc diffusion method for their sensitivity to chosen antibiotics. All of the analysed strains showed α, ß, α+ß, or lh type of haemolysis on plate with 5% sheep blood agar, and had positive reaction in CF and agglutination tests, but were negative in tube coagulase test. These strains showed DNase activity and ability to mannitol fermentation; however, none of the strains produced slime. There were variations in growth on crystal violet agar: examined strains belonged to three types of growth (A, E, and C). The strains were highly susceptible in vitro to oxacillin (99.2%), cefoperazone (95.2%), and amoxicillin with clavulanic acid (93.6%), and highly resistant to neomycin (27.2%), tetracycline (21.6%), and streptomycin (20.8%). In conclusion, it can be stated that intramammary infections in cows can be caused by atypical free coagulase-negative strains of Staphylococcus aureus.

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Changes in antibiotic susceptibility pattern of atypical Staphylococcus aureus strains isolated from cows of the same herd in 2008-2010

100%

Malkinska-Horodyska M., Kubiak J., Lassa H., Malinowski E.

Bulletin of the Veterinary Institute in Puławy

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2012

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tom 56

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nr 2

The isolates of Staphylococcus aureus strains were examined phenotypically by cultural features, tube coagulase test and clumping factor (CF), and genotypically by conventional PCR. The strains had positive reaction in CF test, but were negative in tube coagulase test. The analysed strains from the same cows in each year expressed also nuc and coa genes. About 25% of the strains were examined by the disc diffusion method for their sensitivity to antibiotics. During three years, the strains were highly susceptible in vitro to amoxicillin with clavulanic acid, oxacillin, bacitracin, and cefoperazone (more than 90%), and highly resistant to tetracycline, neomycin, and streptomycin. Forty randomly chosen strains, and eight strains from the same cows in each year were analy sed for minimal inhibitory concentration of penicillin G using microdilution method. An increasing resistance to the penicillin was noted. Moreover, eight strains, the same in each year, were also examined for ß-lactamase production and methicillin resistance. No ß-lactamase producers and no methicillin resistant strains were found using phenotypic and genotypie methods. In conclusion, it can be stated that antimicrobial susceptibility can change from one year to another.

Ograniczanie wyników

2 Bulletin of the Veterinary Institute in Puławy

1 Życie Weterynaryjne

3 Lassa H.

3 Malkinska-Horodyska M.

2 Kubiak J.

2 Malinowski E.

1 Tomaszewska J.

1 2013

1 2012

1 2011

Bakterie najczęściej izolowane z klinicznych postaci mastitis u krów oraz ich wrażliwość na antybiotyki

Phenotypical identyfication of atypical Staphylococcus aureus strains isolated from milk of cows from one herd

Changes in antibiotic susceptibility pattern of atypical Staphylococcus aureus strains isolated from cows of the same herd in 2008-2010