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Laboratory Manual on General and Special Ichthyology

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The manual presents the main methods for studying fish. The general features of the external structure of fish are described: the shape of the body, external organs, the structure and functions of the fins, the types of scales, and the structure of the lateral line. The indicators of morphometric studies of fish with the use of meristic and plastic features according to the classical schemes of measuring fish are also presented. A short description of the statistical method of comparative study of two data samples using the Student's test is given. A method for determining the age of fish by scales is described, as well as a methodology for calculating the annual growth of fish. General information on anatomical structure of fishes is also given. Furthermore, methods of studying the fertility of fish, as well as assessing the nutrition of fish, are described. Particular attention is paid to creating fish preparations: the production of the skeleton of fish, the design of fixed moist preparations and the preparation of a stuffed fish. The presented material has visual illustrations, accompanied by short biological information and forms of tabular material design. The proffered material will be useful for students of the biological and ecological profile, students of natural and agrarian faculties, postgraduate students and young scientists.
Crayfish are valuable invertebrates that are constantly in great demand in all corners of the Earth. Breeding crayfish is not only large-scale production as it can also be arranged in small volumes using pools and installations of closed water supply. Cultivation of crayfish generates profit after a certain period of time, if all biotechnological norms of cultivation are considered. Cultivation of crayfish is a rather promising production, in which it is possible to obtain from 200 to 300% of stable profits during 3–4 years old. The article gives an overview of the technology of growing crayfish, taking into account the characteristics of farming in Ukraine. The basic biotechnological stages of the establishment and operation of the economy are determined, calculations of capital expenditures for the establishment of the economy are presented and the profitability of the project for the production of freshwater crayfish is determined. Cultivation of river crayfish makes it possible to obtain environmentally friendly products that can compete on the markets of Ukraine or EU countries. Also, this way of growing crayfish allows getting stocking material for release in natural reservoirs in order to restore the populations of river crayfish.
Black-striped pipefish Syngnathus abaster Risso, 1827 (Actinopterygii: Syngnathidae) has successfully spread in freshwater ecosystems of Ponto-Caspian basin. Monitoring studies show a steady increase in the number and biomass of pipefish in waters of Dnipro region. It is highly adaptable and breeds in a fresh water. This fact indicates the physiological plasticity of the reproductive system and ability to adapt to new environmental conditions. In the Zaporizke Reservoir pipefish spawns at a temperature of 18.0–22.0°C in the coastal area of the reservoir. Pipefish with a body length of 8.9 to 12.5 cm had gonads with weight of about 0.13 g, and the number of eggs ranged from 26 to 68. The maximum fertility had female 155 mm long and it reached 69 eggs. Size of eggs reached about 1.2±0.05 mm. The histological analysis of the gonads of pipefish and research of histometric measurements of eggs of fish at various stages of development was first conducted. In the period before pipefish spawning, there were eggs in different stages of maturity in the ovaries: oocyte in the phase of primary follicle «С», oocyte in the phase of vacuolation «D₃», oocyte in the phase of trophoplasmatic growth «Е», beginning phase of mature follicle «F». Research of pipefish reproduction has important theoretical and practical significance for resolving issues with the spread of this species and its adaptation to new conditions.
Over the past 50 years, the complete histological study of carp fish gonads was first carried out at Zaporozhian Reservoir. In conditions of environmental transformation of the reservoir the regularities of oogenesis and gonadogenesis, passing sexual cycles were found and the ecology of spawning carp fish was investigated. The adaptive potential of the carp fish reproductive systems, which is expressed through the asynchrony of reproductive products development as well as the functional peculiarities of the fish spawning process, was brought to light. Both environmental assessment and faunal analysis of biodiversity and distribution of young fish of Zaporozhian Reservoir were carried out. The growth and development of the fish larvae dwelling within the industrial agglomerations of Dnipropetrovsk city are found to be under anthropogenic influence. The efficiency of the use of artificial spawning nests for the carp fish spawning was studied, and practical recommendations for their installation and operation in the waters of the reservoir were elaborated.
The educational publication contains microphotographs and description of histological preparations of the structure of marbled crayfish. The above materials may be used both for carrying out laboratory work on disciplines "Histology", "Cytology", "Cell Biology", "Special Practice", and for selfstudy of relevant educational topics. Designed for specialists in the field of hydrobiology and histology, students and graduate students of institutions of higher education who studying in the field of "091 Biology", "207 Water bioresources and aquaculture" and "162 Biotechnology and bioengineering". The publication contains the results of studies conducted by President’s of Ukraine grant for competitive projects Ф75/142 «The reproductive potential of invasive species of Dnieper region reservoirs and their impact on bioproductivity formation» (№ 0118U006319) of the State Fund for Fundamental Research.
The article shows the results of studies on the influence of heavy metal ions (manganese, nickel, lead) on the viability and metabolic enzyme activity of marbled crayfish Procambarus virginalis (Lyko, 2017) (Decapoda). Due to the fact that marbled crayfish got into the reservoirs of the Dnipropetrovsk region in 2015, it was necessary to study the possibilities of its adaptation to environmental factors of reservoirs for further prediction of its distribution or even acclimatization under conditions of toxicological contamination of the ponds of the steppe Prydniprovya. In the experiment with marbled crayfish, chronic effects of various concentrations of heavy metal ions on the physiological state and enzyme activity were investigated. The obtained results showed that among the investigated heavy metals nickel ions influenced the weight indexes and mortality of crustaceans the most negatively. According to the results of the research, significant changes were noted in the individual biochemical parameters of marbled crayfish under the influence of manganese, lead and nickel ions. The most significant changes in the activity of lactate dehydrogenase were detected in muscle tissues affected by manganese and nickel ions. A significant decrease in the activity of succinate dehydrogenase in muscle of marbled crayfish was determined after the action of heavy metal ions. Investigation of changes in the activity of alkaline phosphatase under the influence of the ions of manganese, lead and nickel has its own characteristics, which indicates certain violations in the tissues of cell membranes. Changes in the activity of enzymes were also reflected in the overall protein content. In conclusion, changes in these parameters may indicate a rapid biochemical response of crustaceans to the toxic effects of heavy metals.
It has been investigated the ecological status of the cooling pond of Zaporizhzhya Nuclear Power Plant (NPP) on the hydrochemical and toxicological indicators. The ecological water quality by most hydrochemical and hydrophysical indices corresponds to the class II, category 3, and is characterized as "fairly clean". However, according to the BOD5 index, the water quality is "satisfactory" (class III, category 4). By the contents of both nitrogen of nitrates and phosphorus of phosphates the water quality belongs to the class IV, category 6, as "bad, dirty water". The main anthropogenic pollutants are copper, iron, zinc, and fluorides. The ecological characteristics by their concentrations in the water correspond to the class III, and categories 4–5 of quality, which are "poorly and moderately polluted" water. Accumulation of toxic metals in the surface layer of the reservoir bottom sediments depends on their types, the minimal content is found in sandy sediments, and maximal one is in muddy sediments. The possibility of significant secondary pollution of the water environment by toxic metals is small under the hydrophysical and hydrochemical conditions typical for the cooling pond of Zaporizhzhya NPP. According to the calculations, the value of the average chemical index of ecological quality is "2.5", which characterizes the water of the Zaporizhzhya NPP cooling pond as "good" and "clean".
The article presents the results of biology and ecology studies about the black-striped pipefish Syngnathus abaster (Eichwald, 1831) in the Zaporozhian Reservoir. Quantity and biomass of fish fingerlings and yearlings in different parts of the reservoir was determined. For the first time the reproductive indicators of pipefish in Zaporozhian Reservoir were investigated: the timing of spawning, fish fertility analysis, information on the number of embryos in the brood chamber of males were shown.
The results of studies on the effects of low molecular weight acidic solution peptides on the growth and development of the marbled crayfish artificial cultivation.An increasing weights of juvenile freshwater crayfish under the influence of dietary supplement "Albuvir" drug. With the use of histological methods of research, found the impact of 0.01 % solution of the drug on the state of the marbled crayfish lobules of hepatopancreas and fat cells. Developed a method for growing juvenile freshwater crayfish with "Albuvir", which allows to increase the weight gain of crustaceans on 24.3–27.2 % and reduce the level of cannibalism at 20 %.
Manganese, lead and nickel are heavy metals, which are the primary fresh water toxic contaminants being in the most dangerous class of chemicals. Heavy metals cause functional disorders in the tissues and organs of hydrobionts, affecting their linear and weight indices, reproductive system, digestive and extraction organs. In our experiment on marbled crayfish Procambarus virginalis (Lyko, 2017) we studied the long-term effects of various concentrations of heavy metal ions on the physiological state and histostructure of tissues and organs. According to the results of research, it was found that among the studied heavy metals nickel ions influenced the weight indexes and mortality of crustaceans the most negatively. The results of morphometric studies on marbled crayfish indicate no significant differences between the control group and experimental one, but heavy metals influenced the form of the hepatopancerase lobes and the size of the lumen of the gland. Research on adipocytes of the connective tissue showed a significant difference between the size of cells under the influence of heavy metals. Dimensions of adipocytes fluctuated over a wide range from 144 μm² to 537 μm². In control group the adipocyte area was 406.96 μm². Experimental studies of hemolymph showed that, under the influence of lead ions, a significant 1.4 times increase was observed in the area of hemolymph cells; when manganese and nickel were added, moderate decrease was observed in cells. It was found that under the influence of nickel, the area of round hyalinocytes has reduced by 1.7 times.
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