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Possibility of perchlorate reduction by microbes raises hope for an eco-friendly mode of degradation of this toxic rocket fuel. This study reports 3 isolates (A1, A2 and A3) capable of molybdenum-independent degradation of perchlorate under aerobic conditions. The rate of degradation was the highest when perchlorate concentration was 17 mM, and then 3.2 mM, 4.7 mM and 4.1 mM of perchlorate was reduced by isolates A1, A2 and A3 (respectively) after 72 h at 28°C under aerobic conditions. Presence of perchlorate at a concentration higher than 17 mM resulted in some inhibition of perchlorate reduction. 16S ribosomal RNA gene analysis revealed isolate A1 to be Pseudomonas stutzeri (Proteobacteria) while isolates A2 ad A3 where found to belong to the genus Arthrobacter ( Actinobacteria). The study, apart from demonstrating ribotyping as a rapid method of identification of economically important soil microbes, also raised prospects for using artificial consortia for environmental degradation of perchlorate, without apparent domination of Dechloromonas spp. (a group of microbes known for perchlorate remediation in the environment).
Soil fertility evaluation has been considered as a most effective tool for sustainable planning of a particular region. This study was conducted to determine the soil fertility status of the Agricultural Research Station, Pakhribas, Dhankuta, Nepal. The total 60 soil samples were collected randomly at a depth of 0-20 cm by using the soil sampling auger. For identification of soil sampling points A GPS device was used. The collected samples were analyzed for their texture, pH, OM, N, P₂O₅, K₂O, Ca, Mg, S, B, Fe, Zn, Cu and Mn status following standard methods in the laboratory of Soil Science Division, Khumaltar. The Arc-GIS 10.1 software was used for soil fertility maps preparation. The observed data revealed that soil was dark yellowish brown (10YR 4/4) and yellowish brown (10YR 5/6) in colour, and single grained, granular and sub-angular blocky in structure. The sand, silt and clay content were 56.61±0.97%, 27.62±0.56% and 15.77±0.58%, respectively and categorized as loam, sandy loam and sandy clay loam in texture. The soil was very acidic in pH (4.66±0.07) and very low in available sulphur (0.53±0.11mg/kg), available boron (0.24±0.07mg/kg). The organic matter (1.34±0.07%), total nitrogen (0.09±0.003mg/kg), available calcium (605.70±31.40mg/kg), available magnesium (55.96±4.67mg/kg) and available zinc (0.54±0.22mg/kg) were low in status. Similarly, available potassium (115.98±9.19 mg/kg) and available copper (1.13±0.09 mg/kg) were medium in status. Furthermore, available manganese (36.31±2.82mg/kg) was high, whereas available phosphorus (105.07±9.89 mg/kg) and available iron (55.80±8.89 mg/kg) were very high in status. The soil fertility management practice should be adopted based on the determined status in the field for the sustainable production of crops. The future research strategy should be built based on the soil fertility status of the research farm.
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