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Polyploidization causes ‘genomic shock’ at the genetic and epigenetic levels relative to the parental species. However, few studies have compared the genetic and epigenetic alterations during the process of polyploidization by interspecific hybridization in Brassica species. In the present study, the early generations of hexaploid derived from a triploid hybrid between Brassica napus cv. ‘Zhongshuang 9’ and B. oleracea cv. ‘SWU01’ were developed, and used to detect genetic and epigenetic alterations together with triploid and two parental lines using simple sequence repeats (SSRs) and methylation-sensitive amplified polymorphisms (MSAPs). The derived hexaploids had relatively stable karyotype (69.84% euploid chromosome segregation in S0, 100% and 72.7% hexaploid karyotype in S1 and S2, respectively) and good fertility (88.75% ± 5.43% for pollen fertility, 4.68 ± 3.25 seeds/pod for self pollination and 13.55 ± 3.66 seeds/pod for open pollination), but wide morphologic characters. Compared with the parental lines, the triploid exhibited 13.08% new SSR alleles (17 of 130) and up to 26.45% new MSAP alleles (65 of 246), indicating that interspecific hybridization caused genetic and epigenetic alterations. No change in SSRs and 33.74% alterations in MSAP alleles (83/246) was observed between the triploid and hexaploid S0, suggesting chromosome doubling only caused epigenetic alteration. On average of 39.23% ± 5.78% of DNA methylation alterations and 6.35% ± 3.69% of SSR alterations occurred among the S2, S1, and S0 generations. It indicated that genetic alteration was caused by interspecific hybridization and generation descent of hexaploid, whereas epigenetic alteration resulted from interspecific hybridization, chromosome doubling and generation descent. It is interesting to find stronger epigenetic changes than genetic changes brought on during the processes of hexaploidization between B. napus and B. oleracea. It also found the average genetic distance between hexaploid S2 and hexaploid S1 (SSR 0.056 ± 0.029, MSAP 0.337 ± 0.201) was further than that between hexaploid S1 and hexaploid S0 (SSR 0.023 ± 0.009, MSAP 0.178 ± 0.126), suggesting more genetic variance in the hexaploid S2 generations than hexaploid S1.
The Chinese coking industry (CCI) is currently facing a great challenge on reducing greenhouse gas (GHG) emissions. Our study was set up to assess the GHG emission characteristics of 10 representative coke enterprises and analyse the factors of fuel type, production scale, loading method, oven model, loading rate, etc. The research results showed that fuel gas type, oven model, and loading rate had obvious impacts on the carbon emission intensity of coking plants, while the production scale and the loading method had little effects. The carbon emission intensity of the coking plant using mixed fuel gas was much higher than the plant using coke oven gas as fuel. The carbon emission intensity of the 6.0 m coke oven was 0.7 times higher than that of the 4.3 m coke oven. And the emission intensity of the clean heat recovery coke oven was the highest. An infrequent low loading rate would increase the direct carbon emission intensity signifi cantly. The research results will help the government to compare the emission intensity of the coking industry and make policies about carbon emission intensity reduction.
The Qinshui Basin in Shanxi Province in northern China is currently the largest production area of coal bed methane (CBM) in China. For this study, methane (CH₄) measurements were collected from 113 wellheads to determine primary gas leakage locations. The results indicate that the leakage is primarily from water outlets and tubing; three leakage points accounted for 95.79% of the total measured gas. With respect to measurement variability, the standard deviation for gas measurements of the tubing was the largest at 12.28. Wells with good geological conditions and scientifi c management exhibited very low leakage. In contrast, wells with unfavorable geological conditions and improper management had much higher leakage values. The standard deviation of leakage at the water outlets was the next lowest. The role of different processes and running states had the greatest role in CH₄ leakage. The leakage from horizontal wellheads was the highest, with an average rate of 20.80 l/min, compared to the average of leakage from fl owing wells at 0.88 l/min; this is far below that of the wells that used mechanical gas pumping. T he overall emission factor of the 113 examined wells was 176 kg CO₂-e t⁻¹, which was far greater than the previously reported Australian emission level (11.7 kg CO₂-e t⁻¹).
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