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Enhanced microdialysis of neuropeptides

100%

Pettersson A., Markides K., Bergquist J.

Acta Biochimica Polonica

2001

tom 48

nr 4

An enhanced microdialysis method for neuropeptides is described and some preliminary results of this novel approach are presented. The enhancement is achieved by adding a vehicle (solid support) to the perfusion fluid in order to increase the diffusion coefficient across the membrane and efficiently transport the analytes towards the detector. The microdialysis samples are desalted and then analyzed on an electrospray ionization orthogonal time-of-flight mass spectrometer. The preliminary results show major increase in signal when comparing this new approach of microdialysis with ordinary microdialysis.

Micro-capillary liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry - a powerful tool for peptide and protein identification

76%

Ramstrom M., Palmblad M., Amirkhani A., Tsybin Y. O., Markides K. E., Hakansson P., Bergquist J.

Acta Biochimica Polonica

2001

tom 48

nr 4

In order to be able to study complex biological samples, a micro-capillary liquid chromatography system was coupled to a 9.4 T Fourier transform ion cyclotron resonance mass spectrometer. The setup was tested on a tryptic digest of bovine serum albumin, which resulted in high sequence coverage (> 92 %) of the protein.

Ograniczanie wyników

2 Acta Biochimica Polonica

2 Bergquist J.

1 Amirkhani A.

1 Hakansson P.

1 Markides K.

1 Markides K. E.

1 Palmblad M.

1 Pettersson A.

1 Ramstrom M.

1 Tsybin Y. O.

2 2001

Wyniki wyszukiwania

Enhanced microdialysis of neuropeptides

Micro-capillary liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry - a powerful tool for peptide and protein identification