Wyniki wyszukiwania

1

Spatio-temporal expression of PsAPY1 during germination, differentiation, and organogenesis in pea (Pisum sativum L.Var. Alaska)

100%

Sharma T., Sahi V. P., Morita E. H., Abe S.

Acta Physiologiae Plantarum

|

2013

|

tom 35

|

nr 04

Apyrase (ATP-diphosphohydrolase, EC 3.6.1.5) catalyses the hydrolysis of nucleoside tri- and di-phosphates to nucleoside monophosphates and orthophosphates. In the present study, the spatio-temporal expression of an apyrase gene (PsAPY1) from pea (Pisum sativum L. Var. Alaska) was investigated during the early stages of germination [10 h after imbibition and 16, 35, 62, and 84 h after sowing (HAS)], using non-radioactive mRNA in situ hybridization. At 10 h after imbibition, the PsAPY1 transcript was visible in traces throughout the shoot and root tissues of the embryo. The transcript accumulation became higher by 16 HAS in the tunica layer of the shoot apical meristem, epidermis, vascular bundle, and root tip. At 35 HAS, expression signals became stronger and more widely distributed in the corpus tissues of the growing shoot apical region and in the root; the highest expression was detected in the epidermis, differentiating vascular bundle, and somewhat less in cortex tissues. By 62 HAS, the expression of PsAPY1 was strongest and extensively distributed in the above mentioned regions. At this time, higher expression was also detected in the zones of epicotyl differentiating into plumule and hook. However, at 84 HAS, the expression of PsAPY1 declined in the shoot and root, but was highly expressed in growing lateral root primordia. Germination and growth of seedlings are the physiologically most important stages in the life cycle of plants. The presence of PsAPY1 during these important stages i.e., germination, differentiation, and organogenesis indicates its essential role in growth and development of plants.

2

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Seed source effects on germination, growth, and yield of carrots under natural farming

100%

Katsu Y., Kato K., Abe S., Miyazawa K.

Journal of Horticultural Research

|

2021

|

tom 29

|

nr 2

3

Expression of a gene encoding a MKRN RING zinc finger protein in early germination stages of pea (Pisum sativum L.Var. Alaska) and its possible role in differentiation

100%

Sahi V. P., Arumugam T. U., Morita E. H., Abe S.

Acta Physiologiae Plantarum

|

2011

|

tom 33

|

nr 5

The MKRN gene belongs to the Makorin gene family, which is characterized by a unique array of C3H, RING and a novel Cys-His motif. In the present study we show the spatial expression of MKRN gene in pea (Pisum sativum L.Var. Alaska) tissues at 10 h after imbibition, and 10, 16 and 84 h after sowing, using non-radioactive mRNA in situ hybridization. The results show that the MKRN transcripts were prominently expressed in the shoot apical meristem (SAM), the tunica (region for anticlinal cell division), lateral root primordia and the differentiating zone of root tissues at all stages of germination. The expression of MKRN was more prominent in parenchyma cells of roots at 84 h after sowing. Localization of MKRN transcripts in the SAM after 10 h of imbibition, its induction in the early germination and its continued spatial expression in differentiating zones of the vegetative stage suggest a role for MKRN in organogenesis of pea seedlings. The present results were also consistent with our previous observations in rice seedlings at various stages of germination. We suggest that MKRN plays a crucial role in germination, differentiation and initial organogenesis of pea seedlings.

4

Alternations in phenolic acids content in developing rye grains in normal environment and during enforced dehydration

100%

Weidner S., Fraczek E., Amarowicz R., Abe S.

Acta Physiologiae Plantarum

|

2003

|

tom 25

|

nr 3 suppl.

5

Structure of the coding region and mRNA variants of the apyrase gene from pea [Pisum sativum]

100%

Shibata K., Abe S., Davies E.

Acta Physiologiae Plantarum

|

2001

|

tom 23

|

nr 1

6

Alternations in phenolic acids content in developing rye grains in normal environment and during enforced dehydration

100%

Weidner S., Fraczek E., Amarowicz R., Abe S.

Acta Physiologiae Plantarum

|

2001

|

tom 23

|

nr 4

7

Development of the deep flexor tendons and lumbricalis muscle in the hand and foot: a histological study using human mid-term foetuses

88%

Cho K. H., Kim J. H., Murakami G., Cho B. H., Abe S.

Folia Morphologica

|

2012

|

tom 71

|

nr 3

To revisit foetal development of the deep flexor tendons of the hand and foot, we examined the paraffin-embedded histology of 20 mid-term foetuses at 8–15 weeks of estimated gestational age (35–118 mm crown-rump length or CRL). At 8–9 weeks, in front of the metacarpal bones, the flexor pollicis longus and flexor digitorum profundus (FDP) muscles provided a plate-like, common tendon from which the lumbricalis muscles originated. However, in the foot, we had no evidence of such a common tendon. The flexor pollicis tendon was separated from the common tendon at 9–10 weeks possibly due to mechanical stress from the laterally growing thumb. Notably, at the lumbricalis muscle origins at 10–12 weeks, the FDP and flexor digitorum longus tendons remained undifferentiated and the primitive tenocytes were dispersed from them. The dispersed cells seemed to develop into an interface tissue between the lumbricalis muscle fibre and the deep tendon. In 3 of 5 specimens at 15 weeks, we found an excess number of the FDP tendons (5–7) in the proximal side of the lumbricalis muscle origin. However, the excess tendons dispersed in the lumbricalis muscle origin. The development of the lumbricalis muscle origin might follow the tendon splitting for four fingers. However, conversely, we hypothesised that the developing lumbricalis muscles re-arranged the deep flexor tendons to provide a configuration of “one deep tendon per one finger (or toe)”. The quadrates plantae muscle seemed not to contribute on the re-arrangement. (Folia Morphol 2012; 71, 3: 154–163)

8

Genomic organization and expression profile of a gene encoding makorin RING znic finger protein in germinating pea (Pisum sativum L. var. Alaska) seeds

88%

Abe S., Nakasuji H., Arumugam T. U., Gray T. A., Weidner S. M.

Acta Physiologiae Plantarum

|

2007

|

tom 29

|

nr 1

The makorin RING finger protein gene (MKRN) family encodes proteins with a characteristic array of zinc-finger motifs. In the present study, we characterized the structure and expression of MKRN gene from pea (Pisum sativum L.Var.- Alaska). Pea MKRN cDNA encoded a putative protein, makorin, with four C3H zinc-finger-motifs, one Cys-His putative zinc-finger motif, and one RING zinc-finger motif. The presence of this distinct motif organization clearly established this gene in pea as a true MKRN ortholog. We also isolated RNA from embryonic axes of seeds at various stages of imbibition and germination, including dry seeds, and studied the temporal expression profile of MKRN by RT-PCR. This analysis revealed that MKRN transcripts were present at very low levels, even in dry seeds. The expression of MKRN increased slowly in imbibition period, leveled off in the germination period, and declined in the seedling growth stage. Our reference genes, cytoskeletal actin (ACT) and tubulin (TUB1), were expressed from the early stages of imbibition and steadily increased during germination and plateaued 16–35 h after germination. Similar to MKRN transcripts, TUB1 and ACT transcripts were also detected in dry embryo cDNA. After 62 h of germination, a tissue-dependent expression pattern of MKRN was observed highest in root, moderate in stem, and lowest in plumule. The presence of MKRN transcripts in dry seed and the very early induction of MKRN in germination suggest a developmental role for makorin in pea.

9

Individual variations in the vascular content of retrodiscal tissue in the temporomandibular joint: a study using histological sections of human foetuses and magnetic resonance images of adults without pathology

76%

Yamamoto M., Cho K. H., Choi S. S., Rodriguez-Vazquez J. F., Murakami G., Abe S.

Folia Morphologica

|

2014

|

tom 73

|

nr 2

The vascular content of retrodiscal tissue in the temporomandibular joint (TMJ) plays a critical role in joint function, and its morphology is therefore likely related to TMJ pain. Using histological sections of human foetuses as well as T2-weighted magnetic resonance images (MRI), we measured the vascular content of retrodiscal tissue. MRI showing no pathology in and around the TMJ were obtained from 18 young patients who had been suffering from headache. In 10 small foetuses (12–14 weeks of gestation) as well as 10 larger foetuses (30–37 weeks), the vascular content showed individual variations exceeding 5 times the minimum value (0.24 vs. 0.04 mm² per 1 mm²), but no difference between foetal stages was evident. In the MRI from young adults, the variation was less than twice the minimum value (13.6 vs. 8.7 mm² per 100 mm²). The vascular density appeared to be lower in adults than in foetuses. In both foetuses and adults, the thickness (anteroposterior length) of the tissue did not correlate with the vascular sectional area. These findings suggest that the considerable inter-individual differences evident in the vascular content of foetal retrodiscal tissue may be reduced during further development. (Folia Morphol 2014; 73; 2: 153–158)

10

The influence of abscisic acid on different polysomal populations in embryonal tissue during pea seeds germination

76%

Weidner S., Fraczek E., Romanowska M., Amarowicz R., Abe S., Davies E.

Acta Physiologiae Plantarum

|

2003

|

tom 25

|

nr 1

11

Associations of maize protein bodies with cytoskeleton, membranes, and ribosomes in the endosperm of wild type and opaque-2 mutant

76%

Stankovic B., Abe S., Azama K., Shibata K., Ito Y., Weidner S., Davies E.

Acta Physiologiae Plantarum

|

1999

|

tom 21

|

nr 4

12

Water-deficit-induced changes in cytoskeleton-bound and other polysomal populations in embryonic tissue during triticale caryopsis germination

76%

Kosowska M., Fraczek E., Amarowicz R., Karamac M., Abe S., Weidner S.

Acta Physiologiae Plantarum

|

2004

|

tom 26

|

nr 1

13

Foetal development of the human gluteus maximus muscle with special reference to its fascial insertion

64%

Shiraishi Y., Jin Z. W., Mitomo K., Yamamoto M., Murakami G., Abe H., Wilting J., Abe S.

Folia Morphologica

|

2018

|

tom 77

|

nr 1

The human gluteus maximus muscle (GMX) is characterised by its insertion to the iliotibial tract (a lateral thick fascia of the thigh beneath the fascia lata), which plays a critical role in lateral stabilisation of the hip joint during walking. In contrast, in non-human primates, the GMX and biceps femoris muscle provide a flexor complex. According to our observations of 15 human embryos and 11 foetuses at 7–10 weeks of gestation (21–55 mm), the GMX anlage was divided into 1) a superior part that developed earlier and 2) a small inferior part that developed later. The latter was adjacent to, or even continuous with, the biceps femoris. At 8 weeks, both parts inserted into the femur, possibly the future gluteal tuberosity. However, depending on traction by the developing inferior part as well as pressure from the developing major trochanter of the femur, most of the original femoral insertion of the GMX appeared to be detached from the femur. Therefore, at 9–10 weeks, the GMX had a digastric muscle-like appearance with an intermediate band connecting the major superior part to the small inferior mass. This band, most likely corresponding to the initial iliotibial tract, extended laterally and distally far from the muscle fibres. The fascia lata was still thin and the tensor fasciae latae seemed to develop much later. It seems likely that the evolutionary transition from quadripedality to bipedality and a permanently upright posture would require the development of a new GMX complex with the iliotibial tract that differs from that in non-human primates. (Folia Morphol 2018; 77, 1: 144–150)

Ograniczanie wyników

Spatio-temporal expression of PsAPY1 during germination, differentiation, and organogenesis in pea (Pisum sativum L.Var. Alaska)

Seed source effects on germination, growth, and yield of carrots under natural farming

Expression of a gene encoding a MKRN RING zinc finger protein in early germination stages of pea (Pisum sativum L.Var. Alaska) and its possible role in differentiation

Alternations in phenolic acids content in developing rye grains in normal environment and during enforced dehydration

Structure of the coding region and mRNA variants of the apyrase gene from pea [Pisum sativum]

Alternations in phenolic acids content in developing rye grains in normal environment and during enforced dehydration

Development of the deep flexor tendons and lumbricalis muscle in the hand and foot: a histological study using human mid-term foetuses

Genomic organization and expression profile of a gene encoding makorin RING znic finger protein in germinating pea (Pisum sativum L. var. Alaska) seeds

Individual variations in the vascular content of retrodiscal tissue in the temporomandibular joint: a study using histological sections of human foetuses and magnetic resonance images of adults without pathology

The influence of abscisic acid on different polysomal populations in embryonal tissue during pea seeds germination

Associations of maize protein bodies with cytoskeleton, membranes, and ribosomes in the endosperm of wild type and opaque-2 mutant

Water-deficit-induced changes in cytoskeleton-bound and other polysomal populations in embryonic tissue during triticale caryopsis germination

Foetal development of the human gluteus maximus muscle with special reference to its fascial insertion