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Regeneracja roslin papryki (Capsicum annuum L.) w kulturach in vitro

100%

Borychowski A., Delis M., Niemirowicz-Szczytt K.

Postępy Nauk Rolniczych

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2001

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tom 48

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nr 6

63-74

Pepper (Capsicum annuum L.) is an important crop plant worldwide used as a spice and vegetable. Unlike other species of the Solanaceae family, pepper was a recalcitrant species with regard to its capacity to in vitro regeneration. In many cultivars of pepper, the regeneration was reported via organogenesis of shoot buds from cotyledons and hypocotyl explants, anther culture, somatic embryogenesis and via protoplast culture. However, plant regeneration in the pepper is limited to formation of distorted buds or shoot-like structures that do not produce the normal shoots. Pepper is susceptible to fungal and viral pathogens. One of the solutions to this problem is the development of pathogen-resistant pepper varieties using genetic transformation techniques. Development of an efficient plant regeneration procedure is the first step in optimizing a transformation system.

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Badanie wpływu regulatorów wzrostu na efektywność regeneracji w kulturach papryki z wykorzystaniem regresji logitowej

100%

Delis M., Kobus P., Niemirowicz-Szczytt K., Pietrzykowski R.

Colloquium Biometryczne

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2005

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tom 35

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Differentiation of adventitious buds from Capsicum annuum L. hypocotyls after co-culture with Agrobacterium tumefaciens

100%

Delis M., Garbaczewska G., Niemirowicz-Szczytt K.

Acta Biologica Cracoviensia. Series Botanica

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2005

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tom 47

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nr 1

In vitro organogenesis in hypocotyl explants of the pepper cultivar ‘Bryza’ was induced on MS medium containing 5 mg/l 6-benzyloaminopurine (BAP) and 1 mg/l indole-3-acetic acid (IAA). The hypocotyl explants were then inoculated with Agrobacterium tumefaciens LBA4404(pBI121). After 2 days of culture the first cell divisions were observed in the epidermis and cortex. After 6-7 days, numerous adventitious bud primordia appeared in 58.4% of the explants. In further stages of culture, buds developed into shoots in 8.4% of the explants. Histological analysis revealed hypertrophy and the presence of necrotic cells in the cortex. Necrotic changes were also observed in the vascular bundles. It is likely that culture on a selective medium containing kanamycin and co-culture with Agrobacterium tumefaciens strongly affected the organization of the hypocotyl meristematic tissue, and in consequence brought about necrosis and isolation of the adventitious buds from the vascular bundles.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Colloquium Biometryczne

1 Postępy Nauk Rolniczych

3 Delis M.

3 Niemirowicz-Szczytt K.

1 Borychowski A.

1 Garbaczewska G.

1 Kobus P.

1 Pietrzykowski R.

2 2005

1 2001

Regeneracja roslin papryki (Capsicum annuum L.) w kulturach in vitro

Badanie wpływu regulatorów wzrostu na efektywność regeneracji w kulturach papryki z wykorzystaniem regresji logitowej

Differentiation of adventitious buds from Capsicum annuum L. hypocotyls after co-culture with Agrobacterium tumefaciens