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The aim of the study was to isolate the most common pathogenic strains from calves, cows and the environment, as well as estimating the intensity of (OD) of IgG₁, IgG₂ and IgM reactions to those microorganisms. The study was held in a large commercial dairy farm over the course of 1 year, and included 100 calves and their mothers. OD of IgG₁, IgG₂ and IgM to bacteria isolated from cows, calves and the environment were examined in the investigated serums using the ELISA test. Higher levels of OD were obtained in relation to Salmonella Dublin, Pasteurella multocida, Escherichia coli O26 K6 and Haemophilus som. The above bacteria were also considered to be the most dangerous microorganisms for young calves in the studied farm.
During one year study, 394 stool samples obtained from a random cases of diarrhea were examined for the presence of free shiga-like toxins Stx1 and Stx2 in Vero cells assay. Of 394 stool specimen supernatants tested, 2 (0.5%) gave positive results. The two stool supernatants positive on Vero cell line were wholly capable of being neutralized with the monoclonal antibody to Stx2. Broth cultures of strains isolated from the two positive stool samples were negative for Stxs-production in Vero cytotoxicity assay and by PCR.
Shiga-like toxin-producing (SLTEC) Escherichia coli strains are one of the most important food borne emerging pathogens. One hundred and fifty-seven E. coli strains isolated from 39 children with diarrhea of unknown origin and one hundred and five E. coli strains from 20 healthy children were examined for Shiga-like toxin production in Vero cell line assay. The synthesis of Shiga-like toxin was observed on Vero cell line and confirmed by PCR for one of 262 E. coli strains tested. The shiga-like toxin II-positive E. coli strain was isolated from 2-years old healthy child with no symptoms of gastrointestinal tract infection.
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