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The research was conducted with a view to establishing physiological parameters of the silver fir (Abies alba Mill.) with regard to germination energy, absolute germination and genetic overload produced by silver fir populations. The intention was to identify significant variability within populations and subpopulations, or rather within the two combined, and to give recommendations on the usage and usability of seeds from specific populations. The results form a basis for establishing silver fir genetic overload within five fir populations of central Bosnia and Herzegovina. The results of research on the characteristics such as absolute seed weight, germination energy and absolute germination are within a scope of previous studies. The studied physiological characteristics of silver fir seeds in the central Bosnia area showed distinct individual and inter-population variability when compared to variability of the subpopulations analyzed. The inclusion of non-physiological data (height, DBH, age and the presence of mistletoe) concerning sampled trees proved to be effective new quality of research. No correlation was found between DBH and seed physiological characteristics but increased tree height had a positive effect on seed quality in terms of absolute germination and growth, proportional to tree height. This in turn shows that seeds should be collected only from trees of above average height, because they are less genetically overloaded. The characteristics of healthy but ungerminated seeds from less tall trees displayed lower parameter values, also indicating that seeds should be collected from taller than average trees only. The presence of mistletoe had a very interesting effect of increasing rotten seeds percentage, and therefore reducing seed quality. Accordingly, seed collection should be limited to trees not affected by mistletoe, however further research on this aspect is required.
Three populations of Norway spruce from ecologically extreme environments in Slovenia and Bosnia and Herzegovina were examined for genetic polymorphism. The spruces there grow in specific forest communities (Sphagno-Piceetum) which represent the remnants of the post-glacial vegetation. The aim of the study was to search for similarities in the genetic variation among populations adapted to such conditions. In total, 10 isoenzyme systems involving 16 gene loci were analysed. The results showed differences in genetic differentiation at loci Got-B, Skdh-A and 6-Pgdh-C between the two Slovenian populations and the Bosnian population, but also indicated an interestingly close relationship between the Slovenian population Pohorje and the Bosnian population Nišići.
In the area of the western Balkans, xerothermal broad-leaf forests hold a very special position, the main species being pubescent oak (Quercus pubescens Willd.). The study comprises 36 populations of pubescent oak from Croatia, Bosnia and Herzegovina, Montenegro, Kosovo, Albania and Macedonia. Genetic variability and population diversity was analysed on chloroplast DNA in order to determine to which haplotype an individual or studied population belongs, as well as how they are related. For the detection of one haplotype analysed four sequences (AS, TF, DT and CD) on the chloroplasts DNA. The results of the analysis suggest that there are six different haplotypes of pubescent oak (2, 4, 5, 17, 31, 33) in the studied populations. Two more sub-haplotypes were found in haplotype 5 (a and b), but only in populations occurring in Croatia and Bosnia and Herzegovina. The results suggest the great variability of pubescent oak in the researched area, which is generally characteristic of other species from the area of the Balkan Peninsula. The results obtained by means of cpDNA analysis can help in the formation of seed zones. However, due to the strong selection pressure of unplanned logging, and the possible introduction of herbal material of unknown origin, it is necessary to find a sufficient number of autochthonous entities (i.e., populations of the researched species) as well as new suitable markers for their characterization. The results of this paper constitute a scientifically recognised, partial examination of the origin of the starting forest reproductive material from the broader geographic area, thus serving future projects on its regeneration.
Thirty silver fir populations originating from the putative suture zones of the postglacial recolonization (Slovenia, Bosnia and Hercegovina, Ukraine) were studied using a mitochondrial nad5-4 gene marker. The geographical distribution of mtDNA haplotypes in the Ukrainian Carpathians and their northern foothills indicates a very recent meeting of migration streams arriving from the Romanian Carpathians and Central Europe. In the western part of the Balkan Peninsula, two counterparallel migration streams are the most plausible explanation of the pattern observed. The haplotype typical for the Balkan Peninsula predominates along the Adrian coast, whereas the CentralEuropean haplotype is more represented in the inland.
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