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The first successful report of the in vitro life cycle of Chinese Leishmania: the in vitro conversion of Leishmania amastigotes has been raised to 94% by testing 216 culture medium compound

100%

Li J., Zheng Z.-W., Natarajan G., Chen Q.-W., Chen D.-L., Chen J.-P.

Acta Parasitologica

2017

tom 62

nr 1

Phylogenic analysis of Chinese Leishmania isolates based on small subunit ribosomal RNA (SSU rRNA) and 7 spliced leader RNA (7SL RNA)

100%

Guan W., Cao D.-P., Sun K., Xu J.-N., Zhang J.-R., Chen D.-L., Chen J.-P.

Acta Parasitologica

2012

tom 57

nr 2

The leishmaniases are zoonotic diseases caused by protozoan parasites of the genus Leishmania. Leishmaniases are still endemic in China, especially in the west and northwest froniter regions. To revalue the preliminary phylogenetic results of Chinese Leishmania isolates, we amplified partial fragment of small subunit ribosomal RNA (SSU rRNA) and 7 spliced leader RNA (7SL RNA), then tested the phylogenetic relationships among Chinese Leishmania isolates and their relatives by analyzing SSU rRNA gene sequences and 7SL RNA gene sequences. 19 SSU RNA sequences and 9 7SL RNA sequences were obtained in our study, then analyzed with 42 SSU RNA sequences and 32 7SL RNA sequences retrieved from Genbank, respectively. In the Bayesian analysis of the SSU RNA gene, the isolate MHOM/CN/93/GS7 and the isolate IPHL/CN/77/XJ771 are members of Leishmania donovani complex, while the isolate MHOM/CN/84/JS1 clustered with Leishmania tropica. The other 11 Chinese Leishmania isolates (MHOM/CN/90/WC, MCAN/CN/90/SC11, MHOM/CN/80/XJ801, MHOM/CN/85/GS4, MHOM/CN/84/SD1, MCAN/CN/86/SC7, MHOM/CN/54/#3, MHOM/CN/83/GS2, MHOM/CN/90/SC10H2, MHOM/CN/89/GS6 and MHOM/CN/ 89/GS5) form an unclassified group, defined as Leishmania sp., and the most relative species to this group is L. tarentolae. In the Bayesian analysis of the 7SL RNA gene, 9 Chinese Leishmania isolates also formed an unclassified group with L. tarentolae, including canine isolate 10, MHOM/CN/85/GS4, MHOM/CN/84/SD1, MCAN/CN/86/SC7, MHOM/CN/54/#3, MHOM/ CN/83/GS2, MHOM/CN/90/SC10H2, MHOM/CN/89/GS6 and MHOM/CN/89/GS5. We concluded that: (1) Chinese Leishmania isolates are non-monophyly group; (2) an unclassified group may exist in China, and the most relative species to this group is L. tarentolae; (3) MHOM/CN/84/JS1, which was previously assigned as L. donovani, was most genetically related to L. tropica strain MHOM/SU/74/K27.

Exogenous application of gibberellic acid and ascorbic acid improved tolerance of okra seedlings to NaCl stress

100%

Wang Y.-H., Zhang G., Chen Y., Gao J., Sun Y.-R., Sun M.-F., Chen J.-P.

Acta Physiologiae Plantarum

2019

tom 41

nr 06

Accumulation of salts in soils has become a serious environmental threat for plant growth and causes considerable loss in yield. Okra Abelmoschus esculentus L. is an important crop and sensitive to saline stress. In the present study, to explore methods for growing okra in salty area, exogenous gibberellic acid (GA₃) and ascorbic acid (AsA) were foliage applied on okra seedling under NaCl stress. The results showed that treatment with 100 mM NaCl decreased shoot length, root length, fresh weight, dry weight, contents of chlorophyll pigments and nutrient elements, enhanced levels of electrolyte leakage, H₂O₂, lipid peroxidation and activities of antioxidant enzymes. Treatments with 0.1 mM GA₃ and/or 0.1 mM AsA could alleviated harmful effects of saline stress on okra seedlings by improving growth indicators, increasing contents of chlorophyll and carotenoids, stimulating activities of antioxidant enzymes and decreasing electrolyte leakage, H₂O₂ content and lipid peroxidation. Moreover, concentrations of K, Ca, Mg and Fe in leaves and roots as well as levels of osmo-protectants (proline and soluble protein) increased in response to treatment with GA₃ + AsA in NaCl-stressed okra seedling. Overall, foliar application of GA₃ and/or AsA demonstrated benefits to okra seedlings in salty environments. Combined application of GA₃ and AsA was more effective than sole use of GA₃ or AsA alone.

Ograniczanie wyników

2 Acta Parasitologica

1 Acta Physiologiae Plantarum

3 Chen J.-P.

2 Chen D.-L.

1 Cao D.-P.

1 Chen Q.-W.

1 Chen Y.

1 Gao J.

1 Guan W.

1 Li J.

1 Natarajan G.

1 Sun K.

1 Sun M.-F.

1 Sun Y.-R.

1 Wang Y.-H.

1 Xu J.-N.

1 Zhang G.

1 Zhang J.-R.

1 Zheng Z.-W.

1 2019

1 2017

1 2012

Wyniki wyszukiwania

The first successful report of the in vitro life cycle of Chinese Leishmania: the in vitro conversion of Leishmania amastigotes has been raised to 94% by testing 216 culture medium compound

Phylogenic analysis of Chinese Leishmania isolates based on small subunit ribosomal RNA (SSU rRNA) and 7 spliced leader RNA (7SL RNA)

Exogenous application of gibberellic acid and ascorbic acid improved tolerance of okra seedlings to NaCl stress