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1

Właściwości biologiczne plemników buhaja konserwowanych w różnych typach rozcieńczalników

100%
Demianowicz W., Strzezek J.
Medycyna Weterynaryjna
|
1990
|
tom 46
|
nr 11
It was tried to introduce to a diluent used to freeze bovine men a lipoprotein fraction (LDL) of a low density of egg yolk. The obtained by ultracentrifugation electrophoreallv homogenous fraction of LDL (10%) pasteurized and Ided into a diluent has similar as an yolk egg values otecting plasmolemma of the freezed and thawed spermazoons. A significant differences between the variants of luents containing a full egg yolk and LDL were not und both in a percentage of mobility of spermatozoons, orfological changes in the acrosom and leak of the AspAT iring a process of freezing and after semen freezing, le obtained results point to a possibility to produce diluent for bovine semen of a prolonged stability.
2
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Content available

Biological properties of spermatozoa of Dreissena polymorpha

81%
Bialkowska J., Demianowicz W., Glogowski J.
Folia Malacologica
|
2000
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tom 08
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nr 4
3

Evaluation of CASA system for predicting the motility of zebra mussel [Dreissena polymorpha Pallas] spermatozoa

80%
Bialkowska J., Dietrich G. J., Demianowicz W., Glogowski J.
Electronic Journal of Polish Agricultural Universities. Series Biology
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2004
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tom 07
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nr 2
A computer – aided semen analyzer (CASA) was used for monitoring some motility parameters of zebra mussel sperm after spawning for up to 1,5h at 22°C and after 24 hours of storage at 8°C. The mean percentage of motility (MOT) for sperm shortly after spawning was 70ą2.0 %, VSL = 31.8ą3.0 µm s-1, and VCL= 110.2ą6.4 µm s-1. The motility expressed as LIN was 27.7ą2.0 %, STR=44.5ą4.8 %, ALH=8.7ą2.0 µm, BCF=6.8ą2.0 Hz. The data demonstrate a consistent decrease in VSL (12.8ą 4.0), VCL (57.4ą 5.7), LIN (15.8ą 3.0) and ALH (1.1ą 0.23) values after 24 hours. No significant differences were found for % of MOT, BCF and STR parameters values during the time of experiment. We found CASA analyzer as a proper tool helpful to discerning the quality of zebra mussel semen samples.
4

Glycogen phosphorylase activity and glycogen concentration in developing eggs of Ascaris suum [Nematoda]

80%
Zoltowska K., Dmitryjuk M., Demianowicz W.
Acta Biologica Cracoviensia. Series Zoologia
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1998
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tom 40
5

Activity of glycogen phosphorylase and level of glycogen during development of Ascaris suum [Nematoda] eggs

80%
Zoltowska K., Dmitryjuk M., Demianowicz W.
Acta Biologica Cracoviensia. Series Botanica et Zoologia. Supplement
|
1997
|
tom 39
|
nr 1
6

Determining the viability of zebra mussel [Dreissena polymorpha] spermatozoa and changes in the integrity of its plasma membrane using the fluorescence method

80%
Bialkowska J., Demianowicz W., Glogowski J.
Archives of Polish Fisheries
|
2004
|
tom 12
|
nr 1
The viability of zebra mussel (Dreissena polymorpha) sperm and changes in the integrity of its plasma membrane were examined using combined nucleic fluorescent stains SYBR-14 and propidium iodide. Approximately 97 ± 3% of the sperm in freshly ejaculated zebra mussel semen was stained with SYBR-14 (potentially viable). A statistically significant decrease in plasma membrane integrity was observed after 48 hours (8°C) when 42 ± 11% of the sperm stained as viable. Semen stored at room temperature (20°C) lost its plasma membrane integrity after 4 hours (0% viable). Motility declined from 60 ± 4% (0 time) to 30 ± 3% after 24 hours. The percentage of sperm stained with SYBR-14 was not correlated with sperm motility or the fertility test.
7
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Sexual cells in the reproductive system of female Ascaris suum

80%
Zoltowska K., Lopienska E., Gielwanowska I., Demianowicz W.
Wiadomości Parazytologiczne
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1998
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tom 44
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nr 3
8

Doskonalenie kriokonserwacji nasienia jelenia szlachetnego pobranego metoda sztucznej pochwy

71%
Demianowicz W., Gizejewski Z., Kubiak D., Kowalski R., Glogowski J.
Medycyna Weterynaryjna
|
2008
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tom 64
|
nr 04B
608-612
Cryopreservation of red deer sperm is essential for establishing the biodiversity of this species. The aim of the study was to test four extenders and two freezing methods on the cryosurvival of red deer spermatozoa. Semen collected with an artificial vagina from 4 stags was diluted with compared extenders: A (citrate- -fructose-egg yolk-glicerol), B (Tris-fructose-citric acid-egg yolk-glicerol), C (Triladyl® with egg yolk) and D (Bioxcell®). Sperm, loaded into 0.25 ml straws, was frozen in nitrogen vapor (method L) and in the Minicool 40 PC (method M) cryogenic unit. After thawing the motility of spermatozoa was evaluated subjectively. Viability was assessed using nigrosin-eosin (N-E test), SYBR-14 with propidium iodide (L/D test) and hypoosmotic swelling test (test HOS) to detect membrane integrity. The best post-thaw motility was obtained with the use of extender D (43.0 ± 14.2%). Similarly, more viable spermatozoa (p < 0.05) at N-E and L/D tests (42.4 ± 11.3% and 39.9 ± 14.0% respectively) were preserved in extender D. The lowest results in those tests were received in extender A. The percentage of HOST-responsive spermatozoa was higher (p < 0.05) in method M than in L (19.8 ± 9.8 % vs 14.3 ± 7.6 %), independently of the utilized extenders. In conclusion, the use of extender D in combination with freezing method M significantly improved freezability of red deer spermatozoa.
9

Wpływ zawartości cynku w paszy na jakość nasienia indorów

71%
Glogowski J., Jankowski J., Suszynska-Rzoska E., Demianowicz W., Ciereszko A.
Zeszyty Naukowe. Przegląd Hodowlany
|
2000
|
tom 49
10
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Use biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus)

61%
Stasiak K., Glogowski J., Demianowicz W., Kowalski R., Nowak-Tkaczyk A., Janicki B.
Polish Journal of Veterinary Sciences
|
2014
|
tom 17
|
nr 3
The aim of this study was to use biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus). Twenty-three manually collected ejaculates were analysed for the main indicators of semen quality (sperm concentration and ejaculate volume). Sperm motility and percentage of morphologically normal and abnormal spermatozoa were determined according to the stage of cryopreservation (fresh - measurement A; equilibrated - measurement B; frozen/thawed - measurement C). Furthermore, the seminal plasma and supernatants were analysed after equilibration and freeze/thawing for the activity of the enzymes alkaline phosphatase (ALP), acid phosphatase (AcP), lactate dehydrogenase (LDH) and aspartate aminotransferase (AspAT), and for the activity of acrosin inhibitors (AP). The mean concentration of sperm was 625.1 million/cm3, and ejaculate volume averaged 1.6 cm3. Seminal plasma was characterized by the highest activity of alkaline phosphatase (3.43×103 U/l) and lowest activity of acrosin inhibitors (4.55×103 U/l). After equilibration, the supernatants showed the highest activity of acid phosphatase (94.9 U/l) and after freeze-thawing, they showed a high activity of lactate dehydrogenase (535.8 U/l) and aspartate aminotransferase (577.1 U/l), which indicates that these proteins had leaked from spermatozoa into the extracellular medium during the biotechnique of semen cryopreservation. In addition, several significant relationships were found between some indicators of semen quality and plasma and/or supernatant enzyme activity.
11

Wplyw cynku dodanego do paszy w zroznicowanych dawkach i postaci na jakosc nasienia indorow

61%
Jankowski J., Glogowski J., Suszynska D., Demianowicz W., Koncicki A., Ciereszko A.
Medycyna Weterynaryjna
|
2002
|
tom 58
|
nr 11
895-898
12

Influence of heavy metals and 4-nonylphenol on reproductive function in fish

42%
Popek W., Dietrich G., Glogowski J., Demska-Zakes K., Drag-Kozak E., Sionkowski J., Luszczek-Trojan E., Epler P., Demianowicz W., Sarosiek B., Kowalski R., Jankun M., Zakes Z., Krol J., Czerniak S., Szczepkowski M.
Reproductive Biology
|
2006
|
tom 06
|
nr Suppl.1
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