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The goal of this study is to estimate the effects of low-level laser irradiation (LLLI) on the superovulatory response according to the number of corpora lutea (CL), follicles (F) and the embryo yield. In recent years, while searching for new, more efficient and organic methods to improve superovulatory response and embryo yield with respect to the conventional methods, low-level laser irradiation (LLLI) is a more sensitive and less costly technology that can be used to improve animal reproduction, namely, artificial insemination and the embryo production system. The dairy-cow donors were treated for superovulation with PlusetR, at any time during the oestrus cycle, and the total dose per donor was 700 IU. The first group of the donors (n=25), test group (TG), was irradiated on the sacroiliac area for 180 seconds per day, from the 1st to 11th superovulatory treatment (ST) days in a row, with LLLI in the 870-970-nm wavelength, 65.93 J/cm dose, frequencies in the 20-2000 Hz range and pulse durations commonly in the range of about 1 second. For the second control group (CG) (n=25), the ST was performed without LLLI. After the ST, The mean number of CL in the right side ovaries in the TG was 25.43% (p<0.05) greater than in those of the CG. The number of total recovered and transferable embryos was greater in the TG compared with the CG by 28.97% (p<0.05) and 15.8% (p>0.05), respectively. With respect to conventional methods, LLLI can be used to improve the superovulatory response and embryo yield as a supplementary environment and animal-friendly method of treatment.
The objectives of this study were to define the effect of the milk progesterone (P4) concentration on estrus expression in dairy cows with high milk yield and to identify the effect of milk yield and parity on the milk P4 concentration and cow’s pregnancy after artificial insemination (AI). In this study, 48 clinically healthy cows without reproduction disorders, on day 90-100 after calving were used. At the beginning of estrus and 12 hours after the beginning of estrus, the milk P4 concentration in dairy cows with high milk yield (group 3) was higher than in group 2 (33.66; 22.36%) and in group 1 (51.60; 65.26%) (P < 0.001). The milk P4 concentration in the ≥ 3 lactation cows was higher than the second (13.45%; P > 0.05) and the first (28.28%; P < 0.01) lactation ones (28.28%; P < 0.01). The milk P4 concentration at the beginning of estrus and 12 hours after the beginning of estrus in pregnant and non-pregnant cows was 2.58 and 3.32; 4.20 and 5.00 ng/ml, respectively (P < 0.001). As a result, it was concluded that high progesterone concentration affected the expression of estrus and pregnancy results in dairy cows, and the measuring of progesterone concentration in milk can be used as a non-invasive method to provide detailed information about fertility in high milk yield cows.
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