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1

Investigating the characteristics, evolution and restoration modes of mining area ecosystems

100%
Dong J., Meng L., Bian Z., Fang A.
Polish Journal of Environmental Studies
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2019
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tom 28
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nr 5
The exploitation and utilization of mineral resources bring changes of the regional material cycle and energy flow, resulting in degradation and negative evolution of the local ecosystem. Many countries are committed to researching ecological environment issues and have proposed a series of techniques and methods in recent years, but the comprehensive study of mining area ecosystems and restoration modes is not enough. To further clarify the mining area ecosystem and global restoration, this research analyzed the characteristics and functions of the ecosystem in mining areas, explored its evolution principles, types and processes, and put forward four typical ecological restoration modes. The mining area ecosystem features open, artificial and complex characteristics and special energy and material flows. Its functions mainly refer to the input and output of materials, energy, information and stream of people in the internal and external systems. The ecosystem evolution of mining areas consists of negative evolution and positive evolution in arid and semi-arid areas and humid and semi-humid areas. Overall, four typical restoration modes of mining area ecosystems are featured, including land engineering reclamation, soil improvement and vegetation restoration, secondary wetland construction and ecological self-restoration modes.
2

Simulation of evapotranspiration based on leaf area index, precipitation and pan evaporation: A case study of Poyang Lake watershed, China

76%
Bian Z., Gu Y., Zhao J., Pan Y., Li Y., Zeng C., Wang L.
International Journal of Ecohydrology and Hydrobiology
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2019
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tom 19
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nr 1
3

How exogenous selenium affects anthocyanin accumulation and biosynthesis-related gene expression in purple lettuce

64%
Liu D., Li H., Wang Y., Ying Z., Bian Z., Zhu W., Liu W., Yang L., Jang L.
Polish Journal of Environmental Studies
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2017
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tom 26
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nr 2
We studied the effects of selenite on growth and anthocyanin accumulation in purple lettuce. The results will ascertain which selenite concentration is more advantageous to the purple lettuce and help study the effect of selenite on the molecular mechanism of plant anthocyanin accumulation using the quantitative reverse transcription polymerase chain reaction (qRT-PCR). In this study, the low selenite concentrations (≤ 8μM) could not only promote purple lettuce growth including plant height, leaf area (LA), and fresh weight (FW), but also the anthocyanin contents which may be due to how the selenite affects the UDP-glycose flavonoid glycosyl transferase (UFGT) and flavanone 3-hydroxylase (F3H) genes expression of anthocyanin biosynthesis. Next, on the basis of the obtained results from the preliminary experiments, 8 μM selenite was used to analyze the anthocyanin accumulation with the treatment time prolonged. Although the anthocyanin content of purple lettuce was not reduced on day 24, the control plants were significantly decreased. The reason may be that compared with the control plants, UFGT and F3H genes were markedly up-regulated on day 24. Therefore, the influence of selenium on anthocyanin accumulation and molecular regulation of anthocyanin synthesis is mainly due to the expression levels of the F3H and UFGT gene. It needs to be a further studied.
4

Microarray analysis reveals the role of matrix metalloproteinases in mouse experimental autoimmune myocarditis induced by cardiac myosin peptides

64%
Tang Q., Huang J., Qian H., Xiong R., Shen D., Wu H., Bian Z., Wei X.
Cellular and Molecular Biology Letters
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2007
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tom 12
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nr 2
Autoimmune myocarditis develops after the presentation of heart-specific antigens to autoaggressive CD4+ T cells and after inflammation has infiltrated the tissues. To shed light on global changes in the gene expression of autoimmune myocarditis and to gain further insight into the molecular mechanisms underlying the genesis of myocarditis, we conducted a comprehensive microarray analysis of mRNA using an experimental mouse autoimmune myocarditis model via immunization with α-myosin heavy chain-derived peptides. Of over 39,000 transcripts on a high density oligonucleotide microarray, 466 were under-expressed and 241 over-expressed by ≥ 1.5-fold compared with the controls in BALB/C mouse with autoimmune myocarditis. In this paper, we list the top 50 up-regulated genes related to the immune response. These altered genes encode for leukocyte-specific markers and receptors, the histocompatibility complex, cytokines/receptors, chemokines/receptors, adhesion molecules, components of the complement cascade, and signal transduction-related molecules. Interestingly, matrix metalloproteinases (MMPs) such as MMP-3 and MMP-9 were up-regulated, as further revealed by the reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry assays. This indicates that MMPs may act as major regulators of the cytokine profile. Together, these findings provide new insight into the molecular events associated with the mechanism of the autoimmune genesis of myocarditis.
5
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Abrogation of mitochondrial transcription in smooth muscle cells impairs smooth muscle contractility and vascular tone

51%
Jawien J., Bian Z., Sheikine Y., Olofsson P. S., Pang Y., Edholm T., Dou Y., Metzger D., Hellstrom P. M., Feil R., Hansson G. K.
Journal of Physiology and Pharmacology
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2008
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tom 59
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nr 2
Background: Smooth muscle cells (SMC) constitute the major contractile cell population of blood vessels and inner organs. SMC contraction depends on energy provided by adenosine triphosphate (ATP) catabolism, which can be generated through oxidative phosphorylation in mitochondria or by anaerobic glycolysis. Mitochondrial activity may also modulate smooth muscle tone by biotransformation of vasoactive mediators. Here, we study the role of mitochondrial DNA gene expression for vascular function in vivo. Methods: Since loss of functional mitochondria in SMC may not be compatible with normal development, we generated mice with inducible SMC-specific abrogation of the mitochondrial transcription factor A (Tfam). Deletion of this gene leads to dysfunctional mitochondria and prevents aerobic ATP production in affected cells. Results: Invasive blood pressure monitoring in live animals demonstrated that SMC specific Tfam deletion results in lower blood pressure and a defective blood-pressure response to stress, changes that were not compensated by increased heart rate. The contractility to agonists was reduced in arterial and gastric fundus strips from Tfam-deficient mice. Endothelium-dependent relaxation of arterial strips in response to ACh was also blunted. Conclusion: Our data show that mitochondrial function is needed for normal gastric contraction, vascular tone, and maintenance of normal blood pressure.
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