The level of inbreeding in three subspecies of leopard (Panthera pardus). The estimation of inbreeding level is one of the most important task in captive breeding. The rising of inbreeding coefficient can caused reduction of traits value. It caused deterioration of trait connected with reproduction and immunity. The aim of this paper was to estimate the level of inbreeding coefficient and its changes in time within three leopard Panthera pardus captive populations: Panthera pardus japonensis, Panthera pardus saxicolor and Panthera pardus orientalis. All subspecies are included in European Endangered Species Programme (EEP). The material for analysis was pedigree data taken from the ISIS data base covered information about animals born between year 1915 and 2003. The inbreeding coefficient was calculated using additive matrix method. In the first years (until 1979) average inbreeding was on the high level reachingthe 0.25. Specimens born in the last period (2001- -2003) have smaller inbreeding average on the level 0.043. The percentage of inbred specimens within subspecies was equal to: 13.1% (japonensis), 24.7% (orientalis) and 28.6% (saxicolor).