Epilepsy is a life-threatening disorder that is marked by recurrent seizures. Febrile seizure is a common neurological disorder observed in neonates. In many cases, reducing body temperature can prevent febrile seizure. Transient receptor potential cation channel subfamily M member 8 (TRPM8) is a cation channel that is involved in body thermoregulation. It was reported that M8-B, a TRPM8 antagonist, can reduce body temperature. Thus, we aimed to investigate the effect of M8-B on different experimental seizure models. Eight-day-old male Wistar rat pups were used for induction of febrile seizure. M8-B and diazepam were injected intraperitoneally. The rat pups were then transferred to a heated plexiglas chamber and the latency to the first febrile seizure was measured. In addition, different groups of mice were pretreated with M8-B and received a convulsant dose of pentylenetetrazol (PTZ). Latencies to stages 2 and 4 and duration of stage 5 seizure episodes were measured. Furthermore, the effect of M8-B on electroshock-induced seizures was also investigated and hindlimb extension time was measured. The results showed that M8-B decreased the body temperature of rat pups and increased the latency to the first febrile seizure, implying a significant protective effect. It also induced a significant anticonvulsant effect in PTZ - but not electroshock-induced convulsions. M8-B showed anticonvulsant effects in both febrile- and PTZ-induced seizures. M8-B had a hypothermic effect with significant protective effects on febrile- and PTZ-induced seizures; however, it did not produce similarly protective effects on seizures induced by electroshock.
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.