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In 2007 and 2008 studies aimed to determine the effect of preparation belonging to carbamate compounds (Pirimor 500 WG), organophosphorus compounds (Diazol 500 EW), and quinazolin compounds (Magus 200 SC), on the oxygen consumption rate by adult beetles Anoplotrupes stercorosus were performed. Experiments were carried out under diverse ambient temperatures (14, 19, 24 i 29°C) using two ways of intoxication – contact intoxication or intoxication by ingestion of the biocide. In control insects the ambient temperature affected the oxygen demand only to a small extent. Usually, insecticide preparations which were used, markedly potentiated the oxygen consumption. In those experimental groups significant increases of oxygen consumption as the effect of ambient temperature elevation were noted. The mode of the intoxication influenced oxygen consumption only very slightly. The highest values of oxygen consumption were noted in animals treated by contact intoxication.
The present study focuses on the assessment of porcine endogenous retrovirus (PERV) release from PK15 cells in a time dependent manner. the highest amount of PERV A RNA was detected in PK15 cells after 16 hours of culture. !e highest amount of PERV B RNA was detected in PK15 cells after 20 hours. !e highest amount of both subtypes RNAs was detected in culture medium after 32 hours of culture. the peaks of PERV reverse transcriptase (RT) activity were detected after 28 h of culture in PK15 cells and after 32 hours in the culture medium. the monitoring of PERV release from PK15 cell line may be useful for the evaluation of PERV replication.
Microarray analysis has been used for screening genes involved in specific biological processes. Many studies have shown that restriction factors may play an important role in xenotransplantation safety, but it is still unclear whether porcine endogenous retroviruses (PERVs) may be inhibited by these factors. Therefore, the present study focused on the microarray analysis retroviral restriction factors gene expression in normal human dermal fibroblasts (NHDFs) in response to PERVs. PERV infectivity was analyzed using a co-culture system of NHDFs and porcine kidney epithelial cells (PK15 cell line). Detection of the copy number of PERV A, PERV B DNA and PERV A, PERV B RNA was performed using real-time Q-PCR and QRT-PCR. The expression of retroviral restriction factor genes was compared between PERV-infected and uninfected NHDF cells using oligonucleotide microarray. The up-regulated transcripts were recorded for two differentially expressed genes (TRIM1, TRIM16) with the use of GeneSpring platform and Significance Analysis of Microarrays. In conclusion, our results suggest that the TRIM family may play an important role in innate immunity to PERV infection. These results can allow a better understanding of restriction mechanism of PERV infection and probably design molecularly targeted therapies in the future. Moreover, knowledge of retroviral restriction factor gene expression in human cells may help to uncover strategies for determining their exact function. Microarray analyses seem to be promising in biological and biomedical studies, however, these results should be further confirmed by research conducted at the protein level.
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