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Celem badań było określenie wpływu diety, a konkretnie spożycia tłuszczów nasyconych, na poziom stresu dekompresyjnego. Badania przeprowadzono podczas powietrznych ekspozycji hiperbarycznych do nadciśnienia odpowiadającego głębokości 30 i 60 m. Dietę uczestników ekspozycji badano przy pomocy ankiety, a w oparciu o uzyskane dane wyliczano dzienne procentowe spożycie tłuszczów oraz wartość wskaźnika względnej masy ciała (BMI). Poziom stresu dekompresyjnego badano metodą Dopplera, trzykrotnie po każdej ekspozycji. Stwierdzono istotną zależność pomiędzy zwiększonym spożyciem tłuszczów nasyconych a poziomem stresu dekompresyjnego. Nie stwierdzono natomiast widocznej zależności pomiędzy BMI a poziomem stresu dekompresyjnego.
Sixteen white Wistar female rats were divided into two equal groups. Experimental group received per os 40 mg/kg b.w. of L-arginine, even other day for 2 weeks and were decapitated after 3 weeks of the experiment. Control rats received in the same manner 2 ml of distilled water and were decapitated after 3 weeks of the experiment. The renal lesions observed under electron microscope were of focal character and concerned only the experimental group. The tubules with necrotic cells were observed among normal tubules or single normal epithelial cells of the tubular wall. The boundaries between epithelial cells of the tubule wall were blurred. The mitochondria indicated abnormal structure. Numerous hsosomes and peroxisomes with dark, homogenous content were observed. The rough endoplasmic reticulum had widened channels and was focally completely destroyed. The nucleus of damaged cells was most commonly located in one of the cell poles; its shape was changed and visibly smaller than the nuclei of normal cells. Condensation and peripherally located chromatin were noticed. The lesions observed were characteristic for apoptotic cells.
The aim of the presented study was to determine whether pulmonary barotrauma without pneumothorax affects the parameters of acid-base balance. The experiment was conducted on rabbits divided into experimental and control groups. The animals from the experimental group were subjected to compression in the hyperbaric chamber and pulmonary barotrauma was induced using a method developed by authors. The arterial blood was sampled before the experiment, after anaesthesia and intubation, after injury, and after treatment (in the group undergoing hyperbaric therapy). All parameters of acid-base balance were found to be within the reference ranges. Since the biggest changes were observed after anaesthesia and intubation and not after the injury, it was concluded that pulmonary barotrauma had no significant effect on the parameters of acid-base balance.
The aim of the study was to examine the effects of hyperbaric oxygen on lung aeration on an animal experimental model and compare the obtained results with the anticipated scope of damage to pulmonary parenchyma in humans under the same exposure conditions. The research was carried out on Black Hood rats that were kept in a hyperbaric chamber designed for animals in an atmosphere of pure oxygen and at overpressures of 0.15, 0.2, 0.3, 0.4, and 0.5 MPa for 1, 2 or 4 h. After sacrificing the animals, histopathological specimens were obtained encompassing cross-sections of entire lungs, which were subjected to qualitative and quantitative examination with the use of the 121-point Haug-grid. A statistically significant decrease in pulmonary parenchyma was observed as a result of an increasing oxygen partial pressure as well as with prolonged exposure time. The intensification of changes observed was much higher than expected on the basis of calculations performed with the use of tables.
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