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Understanding population trends of any species is essential for conservation and management. However, due to difficulty in sampling some species, population status of many bat species is poorly understood. In an effort to resolve this issue, especially in light of emerging threats (e.g., white-nose syndrome and wind energy), a national mobile acoustic monitoring protocol, modeled after European programs, was developed to survey summer bat populations in the United States. While the program calls for conducting transects along roadways, some have suggested that waterways may allow for the gathering of more information. Therefore, we quantified species richness and abundance along car and boat transects to identify the most efficient mobile method. Furthermore, to compare the capabilities of mobile acoustic transects to a more traditional and better understood survey method, we compared species density along transects to stationary acoustic detectors. Using sample-based rarefaction, there was no difference at the 95% confidence level in species density (species/sample) between methods, however stationary points accumulated species more quickly than mobile methods. Of the mobile transect methods, car transects had higher diversity indices than boat transects and tended to show slightly higher species density. While over 1.5 times as many calls were recorded and identified along boat transects, there were no clear advantages of boat transects for monitoring bats except for Myotis grisescens. Additionally, car transects were least time consuming, leading us to conclude that car transects are the most efficient mobile acoustic method to monitor species. Mobile acoustic transects can likely monitor 2–4 species in the Eastern United States, including species with no current population monitoring methodology.
Fluorescent paint powders have frequently been used to determine the spatial activity patterns and microhabitat use of small mammals. The time of day that powdered animals were released differs among studies and data used in many studies were collected on the trails of powdered animals released in the morning outside the normal period of activity of many species of small mammals. We tested for differences in the characteristics of fluorescent powder trails of white-footed mice Peromyscus leucopus (Rafmesque, 1818) released using three protocols: night capture-night release, morning capture-morning release, and morning capture-delayed night-release. The night release and morning release protocols were established to replicate the extremes found in the literature. We added the third protocol to evaluate how capturing mice in the morning, holding them in captivity, and releasing them at night affected their use of microhabitat. There were no significant differences in the use of microhabitat between the night release and delayed night-release treatments. However, a significantly greater proportion of the trails of mice released in the morning were in areas of thick cover and under logs than the trails of mice released at night. Because mice released during the day used microhabitat differently than mice released at night, we caution against the interpretation of data on use of habitat collected using the fluorescent powder trails of animals released outside their normal period of activity.
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