Because of an increasing interest in “green” processes for the production of chemicals, researchers are constantly looking for new strains. The natural environment offers a rich isolation source. Strains from natural probes have strong metabolic properties because they must adapt to variable conditions, and they are also able to produce a wide range of metabolites efficiently. This work is a very early report on some capacities of a few bacteria from Hafnia and Citrobacter genera, isolated from environmental probes to 1,3-PD and organic acids (fumaric, succinic, and acetic) production from glycerol. In this report, the predisposition to effective synthesis of 1,3-PD by investigated strains, based on feedback tests and resistance of bacteria to raw material, have been described.