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1

Mast cells and eosinophils in rat mammary gland tumours induced by N-Nitroso-N-methylurea

100%
Kissova V., Sevcikova Z., Revajova V., Herich R., Levkut M.
Bulletin of the Veterinary Institute in Puławy
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2015
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tom 59
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nr 3
The aim of the study was to evaluate the distribution and number of mast cells and eosinophils in rat mammary gland tumours induced by N-Nitroso-N-methylurea. The highest density of mast cells was found in cystic papillary adenocarcinomas of grade II. Eosinophils were detected only in the cystic papillary adenocarcinoma of grades I and II, in non-invasive cribriform adenocarcinoma and comedo-type carcinoma. Mast cell populations were observed perivascularly in the tumour stroma, in the host tumour interface, as well as in necrotic areas of neoplasms. Mast cells were observed to be intact according to their morphological changes, collectively referred to as degranulation. The obtained results indicate that mast cells and eosinophils play an important role in tumour micro-environment formation. The increased density of these cells in experimentally-induced rat mammary gland tumours suggests a poor prognosis in these cancers. Our results also confirmed that rat mammary gland tumours are good models for the study of breast cancers.
2

Polymerase chain reaction detection of caecal bacteria in case of preventive application of Enterococcus faecium EK13 against Salmonella enterica subsp. Enteritidis in chickens

86%
Herich R., Laukova A., Strompfova V., Revajova V., Levkut M., Pistl J.
Journal of Animal and Feed Sciences
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2005
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tom 14
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nr 1
3

Prevalence of anti-microsporidial antibodies in randomly examined dogs in Eastern Slovakia

86%
Herich R., Levkutova M., Kokincakova T., Reiterova K., Hipikova V., Levkut M.
Medycyna Weterynaryjna
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2008
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tom 64
|
nr 05
Sixty-eight dogs were examined for the presence of Encephalitozoon spp. antibodies. Twenty-one dogs (30.9%) were healthy without any clinical signs of diseases. Forty-seven animals (69.1%) developed clinical symptoms of diseases such as chronic otitis externa, conjunctivitis, upper respiratory tract inflammation, status epilepticus, pyodermatitis, skin hypersensitivity, demodicosis, flea allergy. Different detection methods of encephalitozoonosis including IFAT (Indirect Immunofluorescence Antibody Assay), in vitro cultivation, SDS PAGE electrophoresis, Western blot and PCR were applied. There were 33 (48.5%) positive reacting sera to E. cuniculi II (mouse type) antigen using IFAT, including 9 positive samples obtained from clinically healthy dogs. Sixteen samples with the antibody titer equal to 1 : 256 were then tested by Western Blot. Most of the samples reacted with E. cuniculi II and III antigens. The presence of E. intestinalis antibodies was lower and just a few samples reacted with E. hellem antigen. The electrophoretic analysis of the encephalitozoon strains used as antigens confirmed that they differ primarily in the molecular size. The strain of type II (mouse) expressed a double strip at 54 and 58 kDa level. The strain of type III (dog) expressed a wide strip at 59 kDa. E. cuniculi types II and III are more related in protein structure in comparison to the other analyzed strains. When PMP1/PMP2 primers were used in PCR, the size of the amplified product was 268 bp for E. cuniculi and 270 bp for E. intestinalis. A species-specific primer pair for E. cuniculi ECUNF/ECUNR gave a 549 bp fragment and V1/SI-500 primers specific for E. intestinalis gave a 370 bp fragment.
4
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The effect of dietary supplementation of sage plant extract and Enterocin M on the mucus in the the small intestine and caecum in rabbits

72%
Szaboova R., Laukova A., Herich R., Tarabova L., Chrastinova L., Faixova Z., Makova Z., Piesova E.
Polish Journal of Veterinary Sciences
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2021
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tom 24
|
nr 1
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