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The objective of the study was to determine feeding preferences of the khapra beetle (Trogoderma granarium Everts) towards some plant products. The material for the investigations was composed of grain of four barley varieties: Brenda, Krona, Maresi, Rasbet; barley malt, and nutlets of three varieties of buckwheat: Hruszowska, Emka and Kora. The reference material comprised wheat grain of the commercial variety Almari. The results showed that the development of T. granarium larvae on barley grain and malt took longer than on wheat grain. The authors observed that buckwheat nutlets were comparable to barley kernels as a food for the development of the pest. The pest showed more feeding preferences to buckwheat products, grits and meal to barley grain.
The aim of the experiment was to assess the susceptibility of buckwheat grains and products to the infestation by Trogoderma granarium Everts. The observations were conducted on three buckwheat cultivars: Hruszowska, Emka and Kora. The results suggest that buckwheat grains and products as suitable for the development of the first larval stages of Trogoderma granarium as wheat grain and wheat flour. However, extension of the development stage on buckwheat seems to indicate that the seed cover inhibits the capacity of larvae to feed on buckwheat.
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Background. Polyethylenimine (PEI) plays important roles in the pharmaceutical design of non-viral gene delivery systems. Due to a set of unique physicochemical properties this cationic polymer has a great potential in modern gene therapies. Objective. The aim of the present study was to determine the distribution of branched PEI (0.8 kDa) in zebrafish embryos (Danio rerio). Material and methods. Zebrafish embryos at 3 hours post-fertilization (hpf) were incubated with PEI (10 μg/ml) for 24 and 48 hours and studied using the confocal laser microscopy. Results. The obtained results show that PEI effectively distributed into the layers of the chorion and yolk sac in developing embryos due to first 24 hours of exposure. In contrast, PEI was found in the yolk, head, trunk and tail of the embryos due to prolonged treatments (48 hours). Conclusion. The study evidences a high distribution of the branched PEI (0.8 kDa) polymer in the zebrafish embryo tissues.
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