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Functional traits related to environmental divergence in combination with phylogenetic relationship of Picea species

100%

Wang M., Wang J., Zhang A., Zhang X., Sun S., Zhao C.

Dendrobiology

2018

tom 80

Identification of new T1BL.1RS translokation lines derived from wheat (Triticum aestivum L. cultivar "Xiaoyan No. 6") and rye hybridization

100%

Ji J., Wang Z., Sun J., Li J., Zhang X., Wang D., Zhang A.

Acta Physiologiae Plantarum

2008

tom 30

nr 5

Two new T1BL.1RS translocation lines, 48112 and 89121, derived from cross between common wheat (Triticum aestivum L.) cultivar ‘‘Xiaoyan No. 6’’ and rye (Secale cereale L.) cultivar ‘‘German White’’, were developed and identified by using of molecular markers and cytogenetical methods, GISH and FISH. PCR results of primers NOR-R1 specific for rye and Glu-B3 for 1BS detected the presence of 1RS chromatin and absence of 1BS, and primer for gene 1Bx14 in 1BL indicated the existence of chromosome arm 1BL in the two lines. GISH and FISH methods confirmed the replacement of chromosome arm 1BS with 1RS. Further stripe rust resistant test and quality analysis demonstrated that the new 1BL.1RS translocation lines were higher resistant to mixed races of P. striiformis Westend and observed considerable better quality than other popularized T1BL.1RS cultivars in China. The two lines have been used in wheat breeding for high-yield potential and rust resistance.

The role of the GABA-A receptor of the adjacent intact dorsal root ganglion neurons in rats with neuropathic pain

84%

Ran R., Gu J., Fu J., Zhong H., Zhao Y., Gu Y. C., Zhang A., Li G., Wang L., Wang Q.

Acta Neurobiologiae Experimentalis

2014

tom 74

nr 4

We aim to investigate the changes of The y-aminobutyric acid (GABA) signals in the adjacent intact dorsal root ganglion (DRG) and the contribution of these changes to the development and maintenance of neuropathic pain (NPP). After establishment of neuropathic pain model with the lumbar 5 spinal nerve ligation (L5 SNL), the GABA-evoked currents were recorded in the acutely dissociated L4 DRG neurons using whole-cell patch clamp. Moreover, Muscimol or Bicuculline were respectively topically injected to the L4 DRG at the time of nerve injury and post-operative 5 days (POD5). The pain thresholds of hind paw were recorded. We found that the incidence and amplitude of GABA currents significantly decreased in the large and medium L4 DRG neurons after SNL (P<0.05). Furthermore, in the L5 SNL rats, 20 ug Muscimol injected immediately after injury caused a long-lasting attenuation of mechanical allodynia (P<0.05), whereas the thermal hyperalgesia was not alleviated (P>0.05). 0.15 ug Bicuculline further decreased the pain thresholds (P<0.05) and reversed the pain-alleviating effects of Muscimol. However, at POD5, 20 ug Muscimol only exhibited transient alleviating effects (P<0.05). In conclusion, the decrease of inhibition signal mediated by GABA-A receptor in the adjacent intact DRG neurons may be crucial in the development of hyperalgesia in NPP.

Ograniczanie wyników

1 Acta Neurobiologiae Experimentalis

1 Acta Physiologiae Plantarum

1 Dendrobiology

3 Zhang A.

2 Zhang X.

1 Fu J.

1 Gu J.

1 Gu Y. C.

1 Ji J.

1 Li G.

1 Li J.

1 Ran R.

1 Sun J.

1 Sun S.

1 Wang D.

1 Wang J.

1 Wang L.

1 Wang M.

1 Wang Q.

1 Wang Z.

1 Zhao C.

1 Zhao Y.

1 Zhong H.

1 2018

1 2014

1 2008

Wyniki wyszukiwania

Functional traits related to environmental divergence in combination with phylogenetic relationship of Picea species

Identification of new T1BL.1RS translokation lines derived from wheat (Triticum aestivum L. cultivar "Xiaoyan No. 6") and rye hybridization

The role of the GABA-A receptor of the adjacent intact dorsal root ganglion neurons in rats with neuropathic pain