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1

Bacillus anthracis. Potencjalny kandydat na bron biologiczna?

100%
Futoma-Koloch B., Bugla-Ploskonska G.
Ekonatura
|
2008
|
nr 01
10-12
2

Isolation of outer membrane proteins [OMP] from Salmonella cells using zwitterionic detergent and their separation by two-dimensional electrophoresis [2-DE]

81%
Futoma-Koloch B., Bugla-Ploskonska G., Doroszkiewicz W.
Polish Journal of Microbiology
|
2009
|
tom 58
|
nr 4
363-366
We report that using the zwitterionic detergent Zwittergent Z 3-14® to isolate outer membrane proteins (OMPs) from Salmonella O48 is suitable for their separation by two-dimensional electrophoresis (2-DE) in a capillary tube system. Sample preparation is a very crucial step for any bacterial proteomic study. Some modifications were introduced to the 2-DE protocol suggested by O'Farrell and BioRad, which significantly impaired the resolution of proteins. 2-DE analysis of OMPs may be helpful in the interpretation of the variable susceptibility of Salmonella O48 rods to the bactericidal activity of serum.
3

Use of zwitterionic type of detergent in isolation of Escherichia coli O56 outer membrane proteins improves their two-dimensional electrophoresis [2-DE]

81%
Bugla-Ploskonska G., Futoma-Koloch B., Skwara A., Doroszkiewicz W.
Polish Journal of Microbiology
|
2009
|
tom 58
|
nr 3
205-209
Escherichia coli O56 were originally isolated from infected humans. Here it is reported that using the zwitterionic detergent (Zwittergent Z 3-14®) to isolate outer membrane proteins (OMPs) from Escherichia coli O56 is suitable for their separation by two-dimensional electrophoresis (2-DE) using pH 3-10 immobilized pH gradient IPG strips (BIO-RAD).
4

Lizozym - bakteriobojczy enzym o szerokim spektrum zastosowan

81%
Bugla-Ploskonska G., Wojtowicz I., Futoma-Koloch B.
Ekonatura
|
2008
|
nr 07
6-8
5

Rola bialek blony zewnetrznej w oddzialywaniach bakterii Gram-ujemnych z organizmem gospodarza

81%
Bygla-Plaskonska G., Futoma-Koloch B., Doroszkiewicz W.
Postępy Mikrobiologii
|
2007
|
tom 46
|
nr 2
139-152
6

Survival of Proteus mirabilis O3 [S1959], O9 and O18 strains in normal human serum [NHS] correlates with the diversity of their outer membrane proteins [OMPs]

80%
Futoma-Koloch B., Bugla-Ploskonska G., Doroszkiewicz W., Kaca W.
Polish Journal of Microbiology
|
2006
|
tom 55
|
nr 2
Urinary tract infections are frequently caused by Proteus mirabilis strains. In the previous studies there were defined the complete structures of O-polysaccharide parts of lipopolysaccharides from strains: P. mirabilis O3 (S1959), P. mirabilis O9 and P. mirabilis O18. In the present study it was investigated bactericidal effect of normal human serum (NHS) to P. mirabilis strains. We also focused on the diversity of outer membrane proteins (OMPs) being separated on a gel isolated from tested strains. Serial passage of P. mirabilis O18 in 90% normal bovine serum (NBS) contributed to over-expressing some classes of OMPs.
7
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The role of complement activity in the sensitivity of Salmonella O48 strains with sialic acid-containing lipopolysaccharides to the bactericidal action of normal bovine serum

71%
Bugla-Ploskonska G., Futoma-Koloch B., Rybka J., Gamian A., Doroszkiewicz W.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 1
53-62
Sialic acids are important constituents of animal tissue glycoconjugates and are also present in the antigens of some bacterial strains. Capsular polysaccharides with sialic acid (NeuAc) have been extensively studied with regard to sensitivity to the bactericidal action of serum, whereas little is known in this regard about lipopolysaccharides (LPS) which contain NeuAc. Strains of Salmonella 048, able to infect animals and containing the same structures of LPS with NeuAc, were examined for their susceptibility to the bactericidal action of normal bovine serum (NBS). The strains showed varied sensitivity to the bactericidal action of NBS, which indicates that the expression of LPS containing NeuAc residues is not critical for the strains' resistance to the serum's activity. In this study the mechanisms of complement activation responsible for killing serum-sensitive Salmonella 048 rods by NBS were also established. Three such mechanisms were distinguished: activation of the classi- cal/lectin pathways, important (decisive) in the bactericidal mechanism of complement activation, parallel activation of the classical/lectin and alternative pathways, and independent activation of the classical and lectin or the alternative pathway.
8

Modyfikacje struktur komórkowych mikroorganizmów wywoływane działaniem biocydów

71%
Krzyzewska E., Ksiazczyk M., Kedziora A., Futoma-Koloch B., Bugala-Ploskonska G.
Postępy Mikrobiologii
|
2015
|
tom 54
|
nr 4
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Selection and electrophoretic characterization of Salmonella enterica subsp. enterica biocide variants resistant to antibiotics

51%
Futoma-Koloch B., Ksiazczyk M., Korzekwa K., Migdal I., Pawlak A., Jankowska M., Kedziora A., Dorotkiewicz-Jach A., Bugla-Ploskonska C.
Polish Journal of Veterinary Sciences
|
2015
|
tom 18
|
nr 4
The proposed research outlines a serious common concern of Salmonella resistance to antimicrobials following prolonged exposure to the disinfectants (biocides). These phenotypes of bacteria could potentially result in hard to treat infections. Typical for avian sources, biocide sensitive S. enterica subsp. enterica serovars: Typhimurium, Enteritidis, Virchow and Zanzibar and their isogenic biocide-tolerant variants were studied in order to investigate bacteriostatic effect of two commercially available biocide formulations: potassium peroxymonosulfate (P) and dodecylamine based structure (triamine, D). We found that cultivating of the bacteria in the medium supplemented with a blend containing P did not influence their antibiotic susceptibility pattern. In contrast, tolerance of bacteria to D compound resulted in resistance to co-trimoxazole, cefotaxime and ciprofloxacin of which two cefotaxime and ciprofloxacin are used commonly for the treatment of invasive Salmonella infections in humans. The dependency between OMP patterns and the level of Salmonella survival in media containing the biocides was observed merely in serovar Typhimurium. In conclusion, these results suggest that Salmonella strains challenged by prolonged treatment with the disinfectants become resistant to antibiotics, however it depends on Salmonella serovar and the chemical used. This paper also highlights the loop-mediated isothermal amplification (LAMP) as a technique that offers great benefits to microbiological detecting of Salmonella species in the samples.
10

Human complement activation by smooth and rough Proteus mirabilis lipopolysaccharides

51%
Kaca W., Arabski M., Fudala R., Holmstrom E., Sjoholm A., Weintraub A., Futoma-Koloch B., Bugla-Ploskonska G., Doroszkiewicz W.
Archivum Immunologiae et Therapiae Experimentalis
|
2009
|
tom 57
|
nr 5
383-391
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