Wyniki wyszukiwania

1

Somatic embryogenesis and plant regeneration from cell suspension culture of niger (Guizotia abyssinica Cass.)

100%

Naik P. M., Murthy H. N.

Acta Physiologiae Plantarum

|

2010

|

tom 32

|

nr 1

Somatic embryogenesis was achieved from cell suspension cultures of niger (Guizotia abyssinica Cass.). Initially, friable embryogenic calluses were induced from cotyledonary leaves of niger on Murashige and Skoog (MS) agar medium containing 5 µM 2,4-Dichlorophenoxyacetic acid (2,4-D) and 0.5 µM kinetin (KIN). Cell suspension cultures were established by using embryogenic calluses in MS liquid medium containing 5 µM 2,4-D and 0.5 µM KIN. Initiation of somatic embryogenesis and development up to globular stage from embryogenic cell clumps occurred in the liquid medium itself. Thereafter embryogenic cell aggregates were transferred to MS agar medium supplemented with 3 µM KIN for embryo differentiation, whereas maturation of somatic embryos occurred in MS agar medium containing 10 µM abscisic acid.

2

Effects of macro elements and nitrogen source on biomass accumulation and bacoside A production from adventitious shoot cultures of Bacopa monnieri (L.)

80%

Naik P. M., Manohar S. H., Murthy H. N.

Acta Physiologiae Plantarum

|

2011

|

tom 33

|

nr 4

The present work deals with optimization of adventitious shoot culture of Bacopa monnieri for the production of biomass and bacoside A and has investigated the effects of macro elements (NH₄NO₃, KNO₃, CaCl₂, MgSO₄ and KH₂PO₄) and nitrogen source [NH₄⁺/NO₃⁻] of Murashige and Skoog (Physiol Plant 15:473–497, 1962) medium (MS) on accumulation of biomass and bacoside A content. Optimum number of adventitious shoots (99.33 shoots explant⁻¹), fresh weight (1.841 g) and dry weight (0.150 g) were obtained in the medium with 2.09 strength of NH₄NO₃. The highest production of bacoside A content was also recorded in the medium of 2.09 NH₄NO₃, which produced 17.935 mg g⁻¹ DW. The number of adventitious shoot biomass and bacoside A content were optimum when the NO₃⁻ concentration was higher than that of NH₄⁺. Maximum number of shoots (70.00 shoots explant⁻¹), biomass (fresh weight 1.137 g and dry weight 0.080 g) and also bacoside A content (27.106 mg g⁻¹ DW) were obtained at NH₄⁺/NO₃⁻ ratio of 14.38/37.60 mM. Overall, MS medium supplemented with 2.0 × NH₄NO₃ is recommended for most efficient bacoside A production.

3

In vitro regeneration of brahmi shoots using semisolid and liquid cultures and quantitative analysis of bacoside A

71%

Praveen N., Naik P. M., Manohar S. H., Nayeem A., Murthy H. N.

Acta Physiologiae Plantarum

|

2009

|

tom 31

|

nr 4

The major objectives of this study were to investigate an efficient rapid protocol for mass propagation of adventitious shoots of brahmi using semisolid and liquid cultures; and to assess the amount of bacoside A accumulated in the regenerated shoots. Leaf explants were grown in vitro on Murashige and Skoog semisolid medium supplemented with 0.5, 1.0, 1.5, 2.0 and 2.5 mg l⁻¹ 6-benzyladenine or kinetin (KN) or thidiazuron (TDZ) for 4 weeks. Adventitious shoots developed from leaf explants on all cytokinin supplemented media. After 4 weeks of incubation, leaf explants were split into two batches and one set was subcultured on semisolid medium and another set in liquid medium containing same concentration of cytokinins where they have come from. Highest rate of shoot regeneration was observed for explants cultured on medium with 2 mg l⁻¹ KN. The fresh and dry weight of shoots was also highest with this treatment. Liquid cultures were found suitable for proliferation of shoots (155.6 shoots per explant) and they also favored highest biomass accumulation (8.60 g fresh and 0.35 g dry biomass). The bacoside A contents were determined in shoots using HPLC. Analysis revealed that, the contents were highest with shoots regenerated on medium supplemented with 2 mg l⁻¹ KN. The amount of bacoside A was highest in the shoots regenerated in liquid medium (11.92 mg g⁻¹ DW) and it was 2.2-fold higher compared to shoots grown on semisolid cultures.

Ograniczanie wyników

3 Acta Physiologiae Plantarum

3 Murthy H. N.

3 Naik P. M.

2 Manohar S. H.

1 Nayeem A.

1 Praveen N.

1 2011

1 2010

1 2009

Somatic embryogenesis and plant regeneration from cell suspension culture of niger (Guizotia abyssinica Cass.)

Effects of macro elements and nitrogen source on biomass accumulation and bacoside A production from adventitious shoot cultures of Bacopa monnieri (L.)

In vitro regeneration of brahmi shoots using semisolid and liquid cultures and quantitative analysis of bacoside A