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Restriction fragment length polymorphism of mitochondrial DNA and phylogenetic relationships among five species of Indian freshwater turtles

100%

Rohilla M. S., Tiwari P. K.

Journal of Applied Genetics

2008

tom 49

nr 2

167-182

DNA-based identification of species for phylogenetic analysis as well as forensic identification is widely being carried out with the help of polymerase chain reaction (PCR). In this study, a successful effort has been made to identify 5 species of Indian freshwater turtles, including 3 hard-shell turtles (Geoemydidae), i.e. Kachuga dhongoka, K. kachuga and Geoclemys hamiltoni, and 2 species of soft-shell turtles (Trionychidae), i.e. Aspideretes gangeticus and Lissemys punctata punctata, by using a well-optimized PCR-RFLP method. The analysis of nucleotide sequence variations in the PCR-amplified mitochondrial cyt-b genes (encoding cytochrome b) from the 5 species revealed its usefulness in the taxonomic differentiation of these species. On the basis of cyt-b sequence data and the PCR-RFLP pattern, a phylogeny was developed to resolve the genetic relationships between these species, living in the same habitat type. In comparison, the PCR-RFLP of mitochondrial 16S rDNA genes appeared less decisive in analysing phylogenetic relationships or even in species differentiation. Further, the molecular method (PCR-RFLP) developed here is simple, rapid, reliable and reproducible; hence it can be routinely applied for species identification, essential for conservation and management of endangered chelonian species.

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The effect of additional additives on the axillary shoot micropropagation of medicinal plant Aegle marmelos (L.) Correa

80%

Mandal S., Parsai A., Tiwari P. K., Nataraj M.

World News of Natural Sciences

2021

tom 34

India is an immense medicinal plant repository, used in traditional medical treatments. The use of herbal medicine is growing day by day due to toxicity and allopathic drug effects. About 90 percent of the phytochemicals marketed come from wild resources and over 120 major phytochemistry come from plants. Aegle marmelos leaves contained π-sitosterol, aegelin, lupeol, rutin, marmesinine, flavone, glycoside, Oisopentenyl halfordiol, marmeline, and phenylethyl cinnamides. The additional growth promoters show its effect on tissue culture of plant.

Ograniczanie wyników

1 Journal of Applied Genetics

1 World News of Natural Sciences

2 Tiwari P. K.

1 Mandal S.

1 Nataraj M.

1 Parsai A.

1 Rohilla M. S.

1 2021

1 2008

Wyniki wyszukiwania

Restriction fragment length polymorphism of mitochondrial DNA and phylogenetic relationships among five species of Indian freshwater turtles

The effect of additional additives on the axillary shoot micropropagation of medicinal plant Aegle marmelos (L.) Correa