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1

A derivation and evaluation of the Von Bertalanffy equation for describing growth in broilers over time

100%
Darmani Kuhi H., Kebreab E., Lopez S., France J.
Journal of Animal and Feed Sciences
|
2002
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tom 11
|
nr 1
2

Composition and in vitro digestibility of leaves and stems of grasses and legumes harvested from permanent mountain meadows at different stages of maturity

88%
Ammar H., Lopez S., Bochi-Brum O., Garcia R., Ranilla M. J.
Journal of Animal and Feed Sciences
|
1999
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tom 08
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nr 4
599-610
3

Nutritive evaluation of foliage from fodder trees and shrubs characteristic of Algerian arid and semi-arid areas

88%
Bouazza L., Bodas R., Boufennara S., Bousseboua H., Lopez S.
Journal of Animal and Feed Sciences
|
2012
|
tom 21
|
nr 3
The chemical composition and digestibility of foliage from nine browse plant species (Artemisia herba-alba, Atriplex halimus, Acacia nilotica, Acacia horrida, Acacia saligna, Faidherbia albida, Albizia julibrissin, Vicia faba and Punica granatum) grown in arid and semi-arid areas of Algeria were evaluated. Feed components were determined by proximate analysis, whereas phenolic and tannin compounds were analysed by colorimetric procedures and their activity tested using a biological assay. Digestibility was assessed by conventional gravimetric in vitro and in situ methods, and rumen fermentation kinetics were estimated from the in vitro gas production technique. The foliage from Acacia species was found to be a protein-rich fodder for ruminants, although the high lignin and tannin content of some species is an important constraint limiting its digestive utilization in the gastrointestinal tract. The leguminous fodder tree, A. julibrissin, has a high protein content and its foliage is highly digestible owing to its low tannin content. Foliage from P. granatum is a highly digestible browse for ruminants.
4

In situ degradability of soyabean meal treated with Acacia saligna and Atriplex halimus extracts in sheep

76%
Salem A. Z. M., Szumacher-Strabel M., Lopez S., Khalil M. S., Mendoza G. D., Ammar H.
Journal of Animal and Feed Sciences
|
2012
|
tom 21
|
nr 3
The effects of Acacia saligna (AC) and Atriplex halimus (AT) extracts were evaluated on ruminal soyabean meal (SBM) degradability using the nylon bag technique. Samples of SBM were treated with 0, 4, or 8 g of AC or AT extracts per 100 g SBM. Bags were incubated in two cannulated sheep for 2, 4, 6, 8, 12, 24, and 48 h. The chemical constituents (CCs) of extracts was determined using GC-MS. Rate and potential degradability of dry matter (DM) were decreased (P=0.015) to a greater extent than N degradability (P=0.145) with AC and AT doses. DM and N degradation were decreased (P<0.05) by 15% and 29%; 24% and 47% with AC, and 21% and 29%; 23% and 37% with AT at 4% and 8% for DM and N, respectively. The data suggest the possibility of using these extracts as feed additives to reduce ruminal degradability of SBM in ruminant diets.
5

Non-invasive diagnostic of Helicobacter pylori in stools by Nested-qPCR

52%
Taborda M. I., Aquea G., Nilo Y., Salvatierra K., Lopez N., Lopez S., Bresky G., Madariaga J. A., Zaffiri V., Haberle S., Bernal G.
Polish Journal of Microbiology
|
2018
|
tom 67
|
nr 1
The aim of this study was to develop a non-invasive diagnostic test for the detection of Helicobacter pylori in stool samples from digestive symptomatic patients, using a new protocol of nested-qPCR. A total of 143 patients were invited to participate in the study. A gastric biopsy of each patient was collected for Rapid Urease Testing (RUT) and histology by Giemsa stain. A fecal sample for nested-qPCR analysis was also obtained. DNA was extracted from the fecal samples, and conventional PCR followed by qPCR of the ureC gene of H. pylori was carried out. We evaluated the presence of H. pylori, in 103 females and 40 males, mean (± SD) age of 56.5 ± 14.18. The sensitivity of RUT to detect the infection was 67.0% (95% C.I.: 57.2 – 75.8) and specificity was 92.3% (95% C.I.: 76.5 – 99.1). Histology by Giemsa stain, commonly used as a reference for H. pylori detection, showed a sensitivity of 98.6% (95% C.I.: 92.5 – 100.0) and a specificity of 89.7% (95% C.I.: 72.7 – 97.8). In contrast, detection of H. pylori infection in stools by nested-qPCR showed a sensitivity of 100% (95% C.I.: 94.9 – 100.0) and a specificity of 83.9% (95% C.I.: 66.3 – 94.6). Our test, based in nested-qPCR is a better diagnostic alternative than conventional RUT, and is similar to histology by Giemsa stain in the detection of H. pylori, by which the test could be used for non-invasive diagnosis in clinical practice.
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