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Easy PCR screening of Pichia pastoris transformants

100%

Arora D., Chauhan A., Khanna N.

Cellular and Molecular Biology Letters

1998

tom 03

nr 1

A simple protocol for easy PCR screening of P. pastoris transformants is described. In short, the P. pastoris cells are lysed with very small amount of the enzyme Zymolase and the crude cell lysate is directly used in PCR. This protocol needs no tube transfer steps and also obliviates the requirement of freezing the samples at -80°C before PCR screening. Because of a single step screenig, both overall and actual hands on time are considerably reduced.

Stabilization of lyophilization-sensitive antibody-enzyme conjugates

100%

Arora D., Chauhan A. K., Khanna N.

Cellular and Molecular Biology Letters

1998

tom 03

nr 4

A simple method for stabilization of lyophilization or freezing/thawing sensitive enzyme-antibody conjugates is described. The method involves soaking of these reagents directly onto the placebo homoepathic sugar tablets followed by a brief air drying. This solid dry formulation is stable at elevated room temperature and is very convenient for storing or shipping small aliquots of these lyophilzation sensitive reagents.

Ograniczanie wyników

2 Cellular and Molecular Biology Letters

2 Arora D.

2 Khanna N.

1 Chauhan A.

1 Chauhan A. K.

2 1998

Wyniki wyszukiwania

Easy PCR screening of Pichia pastoris transformants

Stabilization of lyophilization-sensitive antibody-enzyme conjugates