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Evaluation of the sensitivity of individual test organisms to residual concentrations of selected types of anticoccidial drugs

100%

Kozarova I., Mate D.

Bulletin of the Veterinary Institute in Puławy

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2000

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tom 44

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nr 2

Anticoccidial drugs with antibiotic or antibacterial activity can be detected by microbiological agar diffusion methods based on the ability of certain drugs to inhibit the growth of sensitive strains of bacteria which act as test organisms and produce inhibition zones. In practice, microbiological agar diffusion methods form the basis of monitoring the residues of inhibitory substances and obtaining primary proof of the presence of residues in foods and raw materials of animal origin. The aim of the present study was to observe the sensitivity of individual test organisms to residual concentrations of selected types of anticoccidial drugs. In our study, four bacterial strains were tested by agar diffusion: Bacillus stearothermophilus var. calidolactis C 953, Bacillus subtillis BGA (at pH 6.0, pH 7.2 and pH 8.0), Micrococcus luteus ATCC 9341 and Bacillus cereus ATCC 11778. The following anticoccidial drug standards were used: amprolium, nicarbazin and furazolidone as chemical compounds, monensin and lasalocid as polyether ionophores, and sulfamethazine and sulfaquinoxaline as representatives of the sulfonamide group. The residual concentrations capable of inhibiting the growth of individual test organisms in our study were compared with maximum residual limits that have been established for these anticoccidial drugs. All anticoccidial drugs were tested using dilutions from 0.0001 mg/ml up to 1 mg/ml.

2

ffects of dietary rosemary extract and alpha-tocopherol on the performance of chickens, meat quality, and lipid oxidation in meat storaged under chilling conditions

81%

Spernakova D., Mate D., Rozanska H., Kovac G.

Bulletin of the Veterinary Institute in Puławy

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2007

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tom 51

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nr 4

3

Aflatoxins and possibilities for their biological detoxification

81%

Laciakova A., Ciconova P., Mate D., Laviak V.

Medycyna Weterynaryjna

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2008

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tom 64

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nr 03

Mycotoxins are secondary metabolites of fungi, which may cause diseases in animals or humans. Aflatoxin B₁ is mycotoxin, which is known to frequently contaminate poorly stored food products destinated for human consuption. In nature, there exist microorganisms for which the aflatoxins are non-toxic. Aflatoxins are degraded through the microorganism’s activity and the obtained products are probably utilized in their metabolism or the microorganisms have ability to bind aflatoxins to their surface. Components of herbs and spices have antiaflatoxigenic properties. They inhibit fungal development and subsequent aflatoxin production. The study reviews literature concerning the detoxification of mycotoxins by microorganisms and components of herbs and spices.

4

Utilization of Monascus purpureus in the production of foods of animal origin

81%

Bakosova A., Mate D., Laciakova A., Pipova M.

Bulletin of the Veterinary Institute in Puławy

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2001

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tom 45

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nr 1

The aim of this study was to use the natural pigment produced by Monascus purpureus as a substitute for nitrites in the production of meat products. Two different concentrations of a Monascus purpureus extract (0.5 g.kg⁻¹ and 0.75 g.kg⁻¹) were tested and compared with the control sample (C) containing a nitrite salting mixture without any addition of Monascus purpureus extract. Based upon the results, poultry ham prepared with half the quantity of nitrite salting mixture and 0.5 g.kg⁻¹ of Monascus purpureus extract showed the most desirable colour, flavour and appearance, the best microbiological parameters and the most suitable salt content.

5

Comparison of the sensitivity of antibiotic residue screening methods to sulphonamide standards and their presumptive identification by para-aminobenzoic acid

81%

Janosova J., Kozarova I., Mate D., Okerman L.

Medycyna Weterynaryjna

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2008

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tom 64

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nr 05

The sensitivity of three microbiological antibiotic residue screening methods - the Four Plate Test (FPT), the Screening Test for Antibiotic Residues (STAR) and the Premi®Test - was compared for the detection of 10 different standards from the sulphonamide (SA) group. Phtalylsulphathiazole (PHT), sulphadimidine (SD), sulphaguanidine (SG), sulphachlorpyridazine (SCHP), sulphamerazine (SRZ), sulphamethoxazole (SMX), sulphanilamid (SAM), sulphanilic acid (SAC), sulphaquinoxaline (SQ) and sulphathiazole (STZ) were tested using the concentrations from 0.05 µg.ml⁻¹ to 1 µg.ml⁻¹. The detection sensitivity of the methods represented by minimum inhibiting concentrations (MIC) of SA standards was evaluated. The MIC of SAs represent the detection limits of methods (LOD) for individual substances. The MIC of SA standards detected by the FPT was 0.2 µg.ml⁻¹ for SMX, STZ, SQ and SRZ, 0.3 µg.ml⁻¹ for SCHP, and 0.4 µg.ml⁻¹ for SD. The MIC of SA standards detected by the STAR was 0.05 µg.ml⁻¹ for SMX, STZ, SQ and SCHP, 0.1 µg.ml⁻¹ for SRZ, and 0.3 µg.ml⁻¹ for SD. No detection sensitivity of either method was observed for SAM, SAC, SG and PHT standards. The MIC of SA standards detected by Premi®Test was 0.05 µg.ml⁻¹ for SMX, STZ, SQ, SCHP, SRZ and SD, 0.1 µg.ml⁻¹ for PHT, 0.2 µg.ml⁻¹ for SAM, and 0.3 µg.ml⁻¹ for SAC and SG. The results of the tests showed that Premi®Test is the most sensitive method to detect Sas, followed by the STAR and the FPT methods. The inhibitory effect of para-aminobenzoic acid (PABA) toward the residual concentrations of SA standards was evaluated. The reference PABA concentrations for the confirmation of the presence of all SAs detected by the FPT and the STAR was 1 µg.ml⁻¹ and 10 µg.ml⁻¹ for the Premi®Test. In the authors’ opinion, the presented concentration of PABA can be recommended for a reliable confirmation of the presence of SA residues even at concentrations that present a potential risk to human health.

6

Action of selected disinfectants on Toxocara canis eggs

71%

Laciak V., Laciakova A., Mate D., Severa J., Pagac M.

Medycyna Weterynaryjna

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2009

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tom 65

|

nr 02

The goal of the work was to monitor the occurrence of helmintologic disorders of dogs in the district of Košice-surroundings. Moreover, the authors wished to evaluate the potential devitalising effect of the following selected chemical substances and disinfective agents: NaOH (5%, 70°C), Savo (10%), Saniten (10%), H1(100%) on eggs of T. canis under laboratory conditions. Using coprologic examination the authors realized that the most common eggs in dog feces are of T. canis (41.4%). Then follow eggs of Trichuris sp. (21.8%), T. leonina (11.5%), eggs from the Ancylostomatidae group (9.2%) and Capillaria sp. (2.3%). Under laboratory conditions, the ovocide effectiveness of NaOH on nonembryonated eggs of T. canis by an exposition of 180 minutes was 23.98 ± 4.33%. The disinfective agent SAVO also had low effectiveness; by an exposition of 180 minutes it devitalized 24.77 ± 5.33% of eggs of T. canis. A higher effectiveness was evidenced by the disinfective agent Saniten. Using the disinfective agent H1, 98.04 ± 1.77% eggs of T. canis were devitalized. Based on the testing of each agent under laboratory conditions the authors can recommend the usage of the disinfective agent Hviezda, as it evidenced the best devitalizing effect on the eggs of T. canis.

7

Utilisation of para-aminobenzoic acid [PABA] for the presumptive identification of sulphadimidine at its residue screening by using the microbiological four-plate test

71%

Kozarova I., Janosova J., Mate D., Pipova M., Jevinova P.

Bulletin of the Veterinary Institute in Puławy

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2005

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tom 49

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nr 1

8

Prevalence of microscopic filamentous fungi in poultry processing plant and concentration of aflatoxin B1 and ochratoxin A in eggs

61%

Laciakova A., Mate D., Pipova M., Pastorova B., Laciak V., Bakosova A.

Bulletin of the Veterinary Institute in Puławy

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2001

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tom 45

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nr 1

The composition of mycoflora in storage rooms, and other rooms in a poultry-processing plant, as well as on the surfaces of egg shells was observed. The concentrations of both aflatoxin B₁ and ochratoxin A were determined in the shell eggs at room temperature and humidity, at a higher temperature and humidity, and in the eggs previously contaminated by Aspergillus flavus. We found that there was a reciprocal correlation between the presence of microscopic filamentous fungi in the air and on the working tables (Cladosporium spp. 45.5%, Penicillium spp. 36.4%, Mucor spp. 9.0%). The penetration of mycotoxins through the egg shell was relatively low and the residue limit of aflatoxin B₁ allowed (5 μg.kg⁻¹) was not exceeded in any sample of egg tested. However, the residue limit of ochratoxin A (20 μg.kg⁻¹) was exceeded in one case.

9

Determination of oxytetracycline residues in milk with the use of HPLC method and two microbial inhibition assays

61%

Jevinova P., Dudrikova E., Sokol J., Nagy J., Mate D., Pipova M., Cabadaj R.

Bulletin of the Veterinary Institute in Puławy

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2003

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tom 47

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nr 1

10

Premi test - screening test for detection of sulphadimidine residues in eggs of laying hens

61%

Marcincak S., Hussein K., Mate D., Kozarova I., Sokol J., Zdolec N.

Medycyna Weterynaryjna

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2006

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tom 62

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nr 02

The study investigated the presence of sulphadimidine residues in eggs after per oral administration of Sulfadimidin PG plv. sol. ad us. vet. (120 mg/hen/day) to laying hens. Premi®Test, a four-plate microbiological method, and HPLC were used to detect sulphadimidine residues. The positive findings of the Four-plate test (FPT) were confirmed by the results of Premi®Test. The absence of sulphadimidine residues was confirmed 72 hours after the last sulphadimidine administration by using the FPT. The presence of sulphadimidine residues was detected by Premi®Test within 8 days and by the FPT within 3 days after the last administration. The FPT test reported false-negative results for five days (kappa < 0.6) as compared with the results of Premi®Test. The study confirmed the conformity of results obtained by both Premi®Test and HPLC (kappa = 0.6).

11

Comparative evaluation of analytical techniques to quantify malondialdehyde in broiler meat

51%

Marcincak S., Sokol J., Turek P., Rozanska H., Dicakova Z., Mate D., Popelka P., Korim P.

Bulletin of the Veterinary Institute in Puławy

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2003

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tom 47

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nr 2

Ograniczanie wyników

Evaluation of the sensitivity of individual test organisms to residual concentrations of selected types of anticoccidial drugs

ffects of dietary rosemary extract and alpha-tocopherol on the performance of chickens, meat quality, and lipid oxidation in meat storaged under chilling conditions

Aflatoxins and possibilities for their biological detoxification

Utilization of Monascus purpureus in the production of foods of animal origin

Comparison of the sensitivity of antibiotic residue screening methods to sulphonamide standards and their presumptive identification by para-aminobenzoic acid

Action of selected disinfectants on Toxocara canis eggs

Utilisation of para-aminobenzoic acid [PABA] for the presumptive identification of sulphadimidine at its residue screening by using the microbiological four-plate test

Prevalence of microscopic filamentous fungi in poultry processing plant and concentration of aflatoxin B1 and ochratoxin A in eggs

Determination of oxytetracycline residues in milk with the use of HPLC method and two microbial inhibition assays

Premi test - screening test for detection of sulphadimidine residues in eggs of laying hens

Comparative evaluation of analytical techniques to quantify malondialdehyde in broiler meat