Wyniki wyszukiwania

1

Rekombinacja integracyjna bakteriofaga λ

100%

Grzesiuk E.

Postępy Mikrobiologii

|

1985

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tom 24

|

nr 3

2

System SOS: Mutagenna odpowiedz komorki na uszkodzenia DNA

100%

Grzesiuk E.

Postępy Biochemii

|

1992

|

tom 38

|

nr 2

59-64

3

The role of mutation frequency decline and SOS repair systems in methyl methanesulfonate mutagenesis

100%

Grzesiuk E.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 2

Methyl methanesulfonate (MMS) is an SN2 type alkylating agent which predominantly methylates nitrogen atoms in purines. Among the methylated bases 3meA and 3meG are highly mutagenic and toxic. The excision of these lesions leads to the formation of apurinic (AP) sites and subsequently to AT-->TA or GC-->TA transversions. The in vivo method based on phenotypic analysis of Arg+ revertants of Escherichia coli K12 and sensitivity to T4 nonsense mutants has been used to estimate the specificity of MMS induced mutations. In the E. coli arg-his-thr- (AB1157) strain MMS induces argE3(oc)-->Arg+ revertants of which 70-80% arise by supL suppressor formation as a result of AT-->TA transversions. The remaining 20-30% arise by supB and supE(oc) suppressor formation as a result of GC-->AT transitions. The level of AT-->TA transversions decreases during starvation. This is a consequence of action of the repair mechanism called mutation frequency decline. This system which is a transcription coupled variant of nucleotide excision repair was discovered in UV induced mutations. We describe the mutation frequency decline phenomenon for MMS mutagenesis. MMS is a very efficient inducer of the SOS response and a umuDC dependent mutagen. In MMS treated E. coli cells mutated in umuDC genes the class of AT-->TA transversions dramatically diminishes. A plasmid bearing UmuD(D')C proteins can supplement chromosomal deletion of umuDC operon: a plasmid harbouring umuD'C is more efficient in comparison to that harbouring umuDC. Moreover, plasmids isolated from MMS treated and transiently starved E. coli AB1157 cells harbouring umuD(D')C genes have shown the repair of AP sites by a system which involves the UmuD'C or at least UmuD' protein.

4

Mutagennosc i kancerogennosc aflatoksyn AFB1.

63%

Pierzynowska J., Grzesiuk E.

Postępy Biochemii

|

1999

|

tom 45

|

nr 4

313-318

5

Heat shock response in gastrointestinal tract

63%

Sikora A., Grzesiuk E.

Journal of Physiology and Pharmacology. Supplement

|

2007

|

tom 58

|

nr 3

43-62

6

Antimutagenic effects of ellagic acid, rutin and psoralen against aflatoxin B1

63%

Pierzynowska J., Grzesiuk E.

Journal of Animal and Feed Sciences. Supplement

|

1998

|

tom 07

|

nr 1

7

Reversion of argE3 ochre strain Escherichia coli AB1157 as a tool for studying the stationary-phase [adaptive] mutations

63%

Nowosielska A., Grzesiuk E.

Acta Biochimica Polonica

|

2000

|

tom 47

|

nr 2

Adaptive (starvation-associated) mutations occur in non-dividing cells and allow growth under the selective conditions imposed. We developed a new method for the determination of adaptive mutations in Escherichia coli. The system involves reversion to prototrophy of the argE3OC mutation and was tested on AB1157 strains mutated in the mutT and/or mutY genes. The bacteria that mutated adaptively grow into colonies on minimal medium plates devoid of arginine (starvation conditions) when incubated longer than 4 days. Using the replica plating method we solved the problem of discrimination between growth-dependent and adaptive argE3Arg+ revertants. Phenotype analysis and susceptibility of the Arg+ revertants to a set of T4 phage mutants create an additional possibility to draw a distinction between these two types of Arg+ revertants.

8

Bacterial DNA repair genes and their eukaryotic homologues: 3. AlkB dioxygenase and Ada methyltransferase in the direct repair of alkylated DNA

63%

Nieminuszczy J., Grzesiuk E.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr 3

Environmental and endogenous alkylating agents generate cytotoxic and mutagenic lesions in DNA. Exposure of prokaryotic cells to sublethal doses of DNA alkylating agents induces so called adaptive response (Ada response) involving the expression of a set of genes which allows the cells to tolerate the toxic and mutagenic action of such agents. The Ada response includes the expression of four genes: ada, alkA, alkB, and aidB. The product of ada gene, Ada protein, is an activator of transcription of all four genes. DNA bases damaged by alkylation are removed by distinct strategies. The most toxic lesion 3meA is removed by specific DNA glycosylase initiating base excising repair. The toxic and mutagenic O6meG is repaired directly by methyltransferases. 1meA and 3meC are corrected by AlkB DNA dioxygenase. The mechanisms of action of E. coli AlkB dioxygenase and its human homologs ABH2 and ABH3 are described in more details.

9

Is the use of antimicrobial drugs in agriculture risky for human health?

51%

Taljanski-Zygmunt W., Grzesiuk E., Zabielski R., Pierzynowski S. G.

Journal of Animal and Feed Sciences. Supplement

|

1998

|

tom 07

|

nr 1

10

Replication and expression of fragments of phage PM2 cloned in Escherichia coli K-12

51%

Grzesiuk E., Rempola B., Fikus M.

Acta Microbiologica Polonica

|

1981

|

tom 30

|

nr 4

11

Spontaneous mutagenesis in exponentially growing and stationary-phase, umuDC-proficient and -deficient, Escherichia coli dnaQ49

51%

Nowosielska A., Nieminuszczy J., Grzesiuk E.

Acta Biochimica Polonica

|

2004

|

tom 51

|

nr 3

Spontaneous mutations arise not only in exponentially growing bacteria but also in non-dividing or slowly dividing stationary-phase cells. In the latter case mutations are called adaptive or stationary-phase mutations. High spontaneous mutability has been observed in temperature sensitive Escherichia coli dnaQ49 strain deficient in 3 '=>5' proofreading activity assured by the £ subunit of the main replicative polymerase, Pol III. The aim of this study was to evaluate the effects of the dnaQ49 mutation and deletion of the umuDC operon encoding polymerase V (Pol V) on spontaneous mutagenesis in growing and stationary-phase E. coli cells. Using the argE3oc =>Arg+ reversion system in the AB1157 strain, we found that the level of growth-dependent and stationary-phase Arg+ revertants was significantly increased in the dnaQ49 mutant at the non-permissive temperature of 37°C. At this temperature, in contrast to cultures grown at 28°C, SOS functions were dramatically increased. Deletion of the umuDC operon in the dnaQ49 strain led to a 10-fold decrease in the level of Arg+ revertants in cultures grown at 37°C and only to a 2-fold decrease in cultures grown at 28°C. Furthermore, in stationary-phase cultures Pol V influenced spontaneous mutagenesis to a much lesser extent than in growing cultures. Our results indicate that the level of Pol III desintegration, dependent on the temperature of incubation, is more critical for spontaneous mutagenesis in stationary-phase dnaQ49 cells than the presence or absence of Pol V.

12

Effect of SOS-induced Pol II, Pol IV, and Pol V DNA polymerases on UV-induced mutagenesis and MFD repair in Escherichia coli cells

51%

Wrzesinski M., Nowosielska A., Nieminuszczy J., Grzesiuk E.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 1

Irradiation of organisms with UV light produces genotoxic and mutagenic lesions in DNA. Replication through these lesions (translesion DNA synthesis, TSL) in Escherichia coli requires polymerase V (Pol V) and polymerase III (Pol III) holoenzyme. However, some evidence indicates that in the absence of Pol V, and with Pol III inactivated in its proofreading activity by the mutD5 mutation, efficient TSL takes place. The aim of this work was to estimate the involvement of SOS-inducible DNA polymerases, Pol II, Pol IV and Pol V, in UV mutagenesis and in mutation frequency decline (MFD), a mechanism of repair of UV-induced damage to DNA under conditions of arrested protein synthesis. Using the argE3→Arg+ reversion to prototrophy system in E. coli AB1157, we found that the umuDC-encoded Pol V is the only SOS-inducible polymerase required for UV mutagenesis, since in its absence the level of Arg+ revertants is extremely low and independent of Pol II and/or Pol IV. The low level of UV-induced Arg+ revertants observed in the AB1157mutD5DumuDC strain indicates that under conditions of disturbed proofreading activity of Pol III and lack of Pol V, UV-induced lesions are bypassed without inducing mutations. The presented results also indicate that Pol V may provide substrates for MFD repair; moreover, we suggest that only those DNA lesions which result from umuDC-directed UV mutagenesis are subject to MFD repair.

13

Mutator specificity of Escherichia coli alkB117 allele

51%

Nieminuszczy J., Janion C., Grzesiuk E.

Acta Biochimica Polonica

|

2006

|

tom 53

|

nr 2

The Escherichia coli AlkB protein encoded by alkB gene was recently found to repair cytotoxic DNA lesions 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) by using a novel iron-catalysed oxidative demethylation mechanism that protects the cell from the toxic effects of methylating agents. Mutation in alkB results in increased sensitivity to MMS and elevated level of MMS-induced mutations. The aim of this study was to analyse the mutational specificity of alkB117 in a system developed by J.H. Miller involving two sets of E. coli lacZ mutants, CC101-106 allowing the identification of base pair substitutions, and CC107-CC111 indicating frameshift mutations. Of the six possible base substitutions, the presence of alkB117 allele led to an increased level of GC→AT transitions and GC→TA and AT→TA transversions. After MMS treatment the level of GC→AT transitions increased the most, 22-fold. Among frameshift mutations, the most numerous were -2CG, -1G, and -1A deletions and +1G insertion. MMS treatment appreciably increased all of the above types of frameshifts, with additional appearance of the +1A insertion.

14

Mutacje adaptacyjne.

51%

Nowosielska A., Grzesiuk E.

Postępy Biochemii

|

2003

|

tom 49

|

nr 2

68-76

15

Physiological and chemical characteristics of antibacterial activity of pancreatic juice

45%

Laubitz D., Zabielski R., Wolinski J., Nieminuszczy J., Grzesiuk E.

Journal of Physiology and Pharmacology

|

2003

|

tom 54

|

nr 2

Attempts were made to find and characterize an antibacterial activity (ABA) factor in porcine pancreatic juice (PJ). Its isolation requires several steps. Since ABA factor was found to be heat resistant, the first step was heating for 30 min at 65°C. Afterwards column chromatography, ethanol precipitation and polyacrylamide gel electrophoresis were involved. Finally, we obtained a pancreatic juice fraction with antibacterial activity against Escherichia coli strain AB1157. In the presence of this fraction the number of living bacterial cells in overnight culture decreased about 10,000 fold and a spot-test gave clearly positive results. The results of analysis suggest that the antibacterial factor is a polypeptide active in a pH range 8.0 - 8.5, that migrates in polyacrylamide gel electrophoresis as a band under 14,000 Da. Mass spectroscopy analysis of active fraction showed high concentration of porcine pancreatic spasmolytic polypeptide (PSP). In conclusion, a polypeptide controlling bacterial homeostasis has been found in the porcine pancreatic juice.

16

Bacterial DNA repair genes and their aukaryotic homologues: 1. Mutations in genes involved in base excision repair [BER] and DNA-end processors and their implication in mutagenesis and human disease

45%

Krwawicz J., Arczewska K. D., Speina E., Maciejewska A., Grzesiuk E.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr 3

Base excision repair (BER) pathway executed by a complex network of proteins is the major system responsible for the removal of damaged DNA bases and repair of DNA single strand breaks (SSBs) generated by environmental agents, such as certain cancer therapies, or arising spontaneously during cellular metabolism. Both modified DNA bases and SSBs with ends other than 3'-OH and 5'-P are repaired either by replacement of a single or of more nucleotides in the processes called short-patch BER (SP-BER) or long-patch BER (LP-BER), respectively. In contrast to Escherichia coli cells, in human ones, the two BER sub-pathways are operated by different sets of proteins. In this review the selection between SP- and LP-BER and mutations in BER and end-processors genes and their contribution to bacterial mutagenesis and human diseases are considered.

17

Alterations in the liver of intrauterine growth retarded piglets may predispose to development of insulin resistance and obesity in later life

39%

Ferenc K., Pietrzak P., Wierzbicka M., Matyba P., Grzesiuk E., Gajewski Z., Zabielski R.

Journal of Physiology and Pharmacology

|

2018

|

tom 69

|

nr 2

18

Intestinal MMC-related electric fields and pancreatic juice control the adhesion of gram-positive and gram-negative bacteria to the gut epithelium - in vitro study

33%

Jankowska A., Wrzesinski M., Laubitz D., Kazimierczak W., Skrzypek H., Bardowski J., Zabielski R., Grzesiuk E.

Journal of Physiology and Pharmacology

|

2008

|

tom 59

|

nr 4

795-810

The adhesion of six different Lactobacillus and Lactococcus and three pathogenic Escherichia and Salmonella strains was studied using Caco-2 cell line. In this in vitro model system the influence of weak electric field (EF) on bacterial adhesion was tested. The EF source was the in vitro reconstruction of spiking potentials recorded in the duodenum of a healthy calf during one myoelectrical migration complex (MMC) cycle. The ability to adhere to Caco-2 cells of bacteria belonging to two groups, Gram-positive lactobacilli and lactococci, and Gram-negative Escherichia and Salmonella differed considerably. The pathogenic bacteria adhered better to well-differentiated Caco-2 cells whereas lactobacilli and lactococci displayed better adhesion to non-differentiated Caco-2 cells. In the presence of MMC-related EF an increased adhesion of Lactobacillus and Lactococcus but not of Salmonella enterica s. Enteritidis and E. coli 269 to Caco-2 cells was observed. Two later strains adhered even less in the presence of EF. The same tendency was found in the presence of pancreatic juice in a cell medium. In conclusion, the myoelectric component of the small intestinal motility, the MMC-related EF, and pancreatic juice may increase the ability of lactic acid bacteria to adhere to GI epithelial cells, creating better environmental conditions for colonization of the intestine and competition with Gram-negative pathogens.

19

Pancreatic secretion differs according to the genotype of growing pigs

33%

Laubitz D., Jankowska A., Nieminuszczy J., Wrzesinski M., Jaworski A., Romanowicz K., Matyjek R., Grzesiuk E., Zebrowska T., Zabielski R.

Journal of Physiology and Pharmacology

|

2006

|

tom 57

|

nr 4

The objective of this study was to investigate the secretion of pancreatic enzymes and antibacterial activity in weaned pigs of three pure breeds, Pietrain, Duroc and Polish synthetic line 990, to look for eventual differences related to the genotype. Six male pigs of each breed, about 24 kg mean body weight, were equipped with chronic pancreatic duct catheters and duodenal cannulas to assess pure pancreatic juice, and jugular vein catheters for blood withdrawal. Pancreatic juice was collected before and after the morning feeding. Protein output and enzyme activities revealed two distinct profiles: strong manifestation of the prandial phase in Pietrain and line 990 pigs, and weak manifestation in Duroc. The antibacterial activity did not follow the enzyme kinetics, and it was the strongest in pancreatic juice from Pietrain pigs. Postprandial insulinaemia was reduced in the order of: line 990>Pietrain>Duroc. A slight (not significant) tendency towards a reduction of leptin after feeding in synthetic line 990 corresponded with elevated secretion of pancreatic enzymes and plasma insulin. The presented results suggest that the prandial secretion of pancreatic juice differs according to genotype, and the differences may be in part related to release of insulin.

Ograniczanie wyników

Rekombinacja integracyjna bakteriofaga λ

System SOS: Mutagenna odpowiedz komorki na uszkodzenia DNA

The role of mutation frequency decline and SOS repair systems in methyl methanesulfonate mutagenesis

Mutagennosc i kancerogennosc aflatoksyn AFB1.

Heat shock response in gastrointestinal tract

Antimutagenic effects of ellagic acid, rutin and psoralen against aflatoxin B1

Reversion of argE3 ochre strain Escherichia coli AB1157 as a tool for studying the stationary-phase [adaptive] mutations

Bacterial DNA repair genes and their eukaryotic homologues: 3. AlkB dioxygenase and Ada methyltransferase in the direct repair of alkylated DNA

Is the use of antimicrobial drugs in agriculture risky for human health?

Replication and expression of fragments of phage PM2 cloned in Escherichia coli K-12

Spontaneous mutagenesis in exponentially growing and stationary-phase, umuDC-proficient and -deficient, Escherichia coli dnaQ49

Effect of SOS-induced Pol II, Pol IV, and Pol V DNA polymerases on UV-induced mutagenesis and MFD repair in Escherichia coli cells

Mutator specificity of Escherichia coli alkB117 allele

Mutacje adaptacyjne.

Physiological and chemical characteristics of antibacterial activity of pancreatic juice

Bacterial DNA repair genes and their aukaryotic homologues: 1. Mutations in genes involved in base excision repair [BER] and DNA-end processors and their implication in mutagenesis and human disease

Alterations in the liver of intrauterine growth retarded piglets may predispose to development of insulin resistance and obesity in later life

Intestinal MMC-related electric fields and pancreatic juice control the adhesion of gram-positive and gram-negative bacteria to the gut epithelium - in vitro study

Pancreatic secretion differs according to the genotype of growing pigs