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Celem badań była ocena wybranych cech jakościowych takich, jak: wartość sensoryczna, zawartość kwasu L(+) mlekowego, kwasowość miareczkowa oraz przeżywalność 10 szczepów bakterii z rodzaju Bifidobacterium w ciągu 21 dni przechowywania w nieukwaszonym i ukwaszonym mleku bifidusowym. W wyniku przeprowadzonych badań stwierdzono, że jedynie chłodnicze warunki przechowywania mleka bifidusowego zapewniają utrzymanie prawidłowej jakości produktu. Wszystkie badane szczepy produkują prawie wyłącznie izomer L(+) kwasu mlekowego. Udział procentowy tego kwasu w całej puli kwasu mlekowego wynosi po 21 dniach niezależnie od warunków przechowywania powyżej 90%.
The article presents the results of the beet juice fermentation by Lb. brevis Lock 0944. The obtained product was characterised by desirable organoleptic features and microbiological stability It was concluded that the appliance of the starter cultures of lactic fermentation bacteria to production of galenical functional food assures that the obtained product will have a definite organoleptic features, that the risk of development of morbific bacteria is reduced, and allows to reduce applied preservatives.
Our study aimed to indicate the possibility of the application of 5 strains of Lactobacillus plantarum and 1 strain of Lactobacillus brevis isolated from plant material for reducing the count of Salmonella Senftenberg W775 in mixed culture. All the tested strains completely inactivated growth of Salmonella Senftenberg rods during 48 h of common culture. The strongest antagonistic activity was shown by strain Nos. 11, 13, and 15, which after 24 h of co-culture totally eliminated the pathogen cells. No effect of the pathogen on the growth dynamics of lactic acid bacteria was observed.
Gut microbiota was analyzed in children, aged 6–18 months and su$ering from atopic dermatitis before and after 3 month supplementation of their diet with Lactobacillus casei DN – 114001 in a dose of 10⁹ cells daily. On completion of this period the total number of fecal Lactobacillus sp. cells decreased from 7.86 Log₁₀ CFU/g to 6.40 Log₁₀ CFU/g. After the next 5 months (without dietary supplementation with the probiotic bacteria) the level of Lactobacillus sp. cells was maintained at the latter value. During the dietary supplementation with the probiotic strain, the level of Bifidobacterium cells was maintained at 6.15–6.89 Log₁₀ CFU/g while after 5 months it decreased to 5.57 Log₁₀ CFU/g. The population of Clostridium sp. was reduced after 3 months of dietary supplementation from 6.49 to 5.83 Log₁₀ CFU/g and was maintained at the latter level during the next 5 months. The dietary supplementation had no effect on populations of Bacteroides sp., Enterococcus sp. and Enterobacteriaceae. Supplementation of children who developed atopic dermatitis with the preparation of Lactobacillus casei DN – 114001 positively affected their gut microbiota in terms of bifidobacteria and clostridia populations.
Background. Selenium is an element of very great importance for the proper functioning of the human body, mainly due to its antioxidant properties. Selenium exhibits a preventive effect in the case of cardiovascular disease, the immune system, male infertility and inhibits the toxic action of other agents. Selenium is important for Hashimoto’s disease. Intake of selenium in the diet slows the aging process. The biological and toxicological effects of selenium strongly depend on its chemical form. Some organisms for example: plant, yeast, are capable of metabolizing low bioavailable selenium compounds (inorganic selenium) into its high bioavailable forms (organic selenium). Objective. The aim of this study was to investigate the bio-transformation of selenium by Lactobacillus bacteria towards the characterisation of selenium metabolites. Material and Methods. The speciation of selenium was evaluated by high performance liquid chromatography with inductively coupled plasma mass spectrometry detector. The extraction of selenium species from lyophilized bacteria was executed with water, the mixture of lipase and protease, as well as lisozyme and sodium dodecyl sulphate. Results. All investigated bacteria strains cultivated in the presence of Na2SeO3 effectively uptake selenium. Surprisingly, none of the applied extraction media exhibited a strong power to release the majority of the uptaken selenium compounds. Thus a maximum of 10% of the selenium was extracted from bacteria exposed to the enzymes. However, it was found that Lactobacillus bacteria are able to metabolize inorganic ions of selenium (IV) into Se-methionine, Se-methyloselenocysteine and other unidentified forms. Conclusions. The study confirmed the ability of probiotic bacteria to biotransform inorganic selenium into its organic derivatives. Therefore, Se-enriched bacteria can be considered as an addition to the functional food.
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