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The potential involvement of activated oxygen species by submergence stress was studied in two Malaysian rice cultivars, MR219-4 and MR219-9, and cultivar FR13A that is known to be tolerant to submergence. Seedlings of these three rice cultivars were subjected to different submergence periods (4, 8, and 12 days). Under 8 days of complete submergence, FR13A cultivar showed higher lipid peroxidation in terms of malondialdehyde level and activities of antioxidative enzymes, superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) when compared to the MR219-4 and MR219-9 cultivars. MR219-9 showed higher SOD, APX, and GR activities after 12 days of submergence. The levels of SOD activity indicated that detoxification of O₂⁻ to H₂O₂ was maintained at a stable level throughout the submergence stress until up to 8 days and increased rapidly at 12 days of submergence. The results indicated that tolerance to submergence in rice is associated until 8 days submergence for MR219-4 and FR13A cultivars. These findings suggested that tolerance to submergence stress in rice might be proven by increased the capacity of antioxidative system. In addition, CAT activity has much higher affinity for scavenges H₂O₂ than APX. Therefore, ascorbate glutathione cycle might be more efficient to scavenge H₂O₂.
The potential agricultural lands are falling prey to salinity in the world over including Pakistan. The limited water supply is also becoming a serious problem to feed the humans and livestock production. Therefore, research studies were undertaken to enhance the growth and development of sunflower (Helianthus annuus L.) on saline soils to increase productively of crop. The treatments consisted of (a) two lines of sunflower (Hysun-33 and LG-56-63), (b) two levels of salinity (0, 120 mM (NaCl)) and (c) two levels of salicylic acid (0, 200 mg L-1) and were arranged in a completely randomized design with four replications. The results showed that biological yield was significantly reduced due to imposition of salinity at the rate of 120 mM (NaCl) on both sunflower lines (Hysun -33, LG 56-63). The stem length was also reduced due to decrease in biological yield in response to salinity. However, the exogenous application of salicylic acid at the rate of 200 mg L-1 mitigated the adverse effects of salts and improved the biological yield and stem length under saline and non-saline environments. The quantity of chlorophyll (SPAD) values were impacted negatively in response to salt stress, however, the phenomenon was recovered by foliar spray of salicylic acid. The nutrient concentration of K+, Cl- and Na+ were altered because of presence of excess quantity of NaCl in the substrate. The translocation of K+ ion was reduced substantially, while higher amount of Na+ and Cl- ions were absorbed, thus creating ionic imbalance in the plant system. The foliar spray of salicylic acid (200 mg L-1) enhanced the uptake of K+ from the soil medium. The salicylic acid proved a potential phytoprotectant to mitigate the adverse effects of salinity and thereby improving the physiological and biochemicals attributes, stem length and also enhanced uptake of K+ ion while depressing Na+ and Cl- ions in plant system.
Silymarin, a potential phytochemical compound obtained from the seeds of Silybum marianum plant has been used as a hepatoprotective agent for more than a decade. So far, eight active components of silymarin flavonolignans have been identified, among which silibinin has been proven the most active. However, it had poor oral bioavailability due to extensive phase II metabolism, low permeability across intestinal epithelial cells, low aqueous solubility, and rapid excretion in bile and urine. Therefore it becomes necessary to understand all its formulation and analytical aspects from past to present, including all of its possible future prospects. In modern research scenario, nanotization strategies of drugs has served as a potential approach to enhance solubility, bioavailability and to develop a robust formulation. Several approaches have been utilized previously to enhance the solubility and bioavailability of silymarin to provide it a robust strength against physical, chemical, and environmental degradation. Nanoscale formulations such as nanoemulsion, nanosuspension, liposomes, and solid–lipid nanoparticles can be used to enhance solubility and to target them to desired cells with minimum harm to normal cells. However, many other approaches exist such as dendrimers, ceramic nanoparticles, and carbon nanotubes, which serve as a great vehicle in drug delivery to transport medicament at target sites. Therefore, the purpose of this review was to develop a better understanding of the problems associated with silymarin and approaches to overcome the difficulties to develop a better and stable formulation for food and pharmaceutical applications.
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