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The potential differentiations in litter chemistry among native and non-native trees are poorly understood. We compared the chemical composition of leaf litterfall of 11 exotic tree species, e.g. coniferous: Abies cephalonica, A. grandis, A. procera, Chamaecyparis pisifera, Pinus peuce, Pseudotsuga menziesii, Thuja plicata, and deciduous: Acer rubrum, A. saccharum, Betula alleghaniensis and Quercus rubra, with that of a native European conifer, Pinus sylvestris (as reference to coniferous species) anda mixture of native European Quercus robur, Carpinus betulus, Tilia cordata, T. platyphyllos and Corylus avellana leaves (as a reference mixture of deciduous species). We found significant differences among the species studied in nitrogen and carbon content in needles/leaves, C/N ratio, as well as total soluble phenolic compounds (TPh) and total nonstructural carbohydrates (TNC) content, including soluble carbohydrates and starch. However, we found no clear differentiation of exotic from native tree species in the analyzed elements and metabolites. Among the exotic coniferous tree species, P. menziesii stood out among the species studied – fallen needles of this species were characterized by relatively high TPh and TNC content. The relationships between TPh and TNC content in leaf and needle litter among tree species were similar among two consecutive years. For deciduous tree species, the tendency of higher TPh content and C/N ratio in leaves falling earlier (September; leaves of sun-type) than later (November; leaves of shade-type) was more distinct than in coniferous tree species. Generally, we cannot see any special differences in the levels or mutual quantitative relationships of the chemical compounds studied in fallen needles/leaves of exotic tree species in comparison with native tree species.
Activity of soil dehydrogenase (DHA) was measured in the mineral soil in a forest stand of 15 to 16-year-old Scots pine (Pinus sylvestris L.) from geographically diverse populations, as an indicator of biological activity of soil microorganisms, in a provenance experiment in Poland. The pine populations originated from six European countries (Sweden, Russia, Latvia, Poland, Germany, France) and differed widely in aboveground biomass and productivity. Soil DHA during two growing seasons showed pronounced seasonal variability, which was significantly related to the fine root concentration of nonstructural carbohydrates. Higher DHA was found in soil under canopies of the central and southern European populations than in those from more northern parts of the Scots pine range. Significant positive correlation between soil DHA and aboveground tree biomass suggest that these patterns most likely resulted from differences in carbon dynamics and productivity among populations.
The process of soil degradation and destabilization of forest ecosystem by industrial pollution is frequently associated with mobilization of toxic Al³⁺ ions. Both these processes exert a negative influence on tree root systems and may even result in the decline of whole forest stands. One-year-old seedlings of silver birch (Betula pendula Roth.) grown in pots were treated with a range of aluminum sulfate concentrations in order to test the effects of Al on growth, root structure, content of phenolic compounds and mineral nutrition of roots and foliage. Plants exposed to Al concentrations exceeding 50 mg Al dm⁻³ had reduced growth, root structure and nutrient uptake were affected, and a substantial increase of Al concentration occurred in foliage and roots. Concentration of several elements in the foliage and roots declined with increasing Al concentration, including Mg and Ca, and to a lesser extent, P, K, and Na. Most root traits such as root mass or root growth rate were more strongly affected by Al than the foliage. Changes in root Ca, Al and Ca:Al ratio, and root morphology were detected at the lowest Al concentration (50 mg Al dm⁻³) indicating usefulness of these traits as early indicators of adverse aluminum effects on plants.
At the end of July 1997 a premature shedding of one- and two-year-old foliage of Scots pine (Pinus sylvestris L.) was observed in central Poland. We examined the etiology and physiological consequences of this needle shedding event in a 15-year-old Scots pine plantation with diverse populations originating from Sweden, Russia, Latvia, Poland, Germany and France. On average, trees lost 20% of two-year-old foliage, with a local population from Poland having the highest needle loss (28%) and the lowest in a population from France (13%). However, differences among populations in needle loss were only marginally significant (p = 0.1). Phytopathological observations excluded biotic factors as responsible for needle loss. Analysis of thermal conditions in 1997 suggest that premature needle shedding may originate from the combination of winter physiological drought and unusually high (up to 35°C) air temperatures and low precipitation in late spring. We found that winter drought significantly affected the foliage by reducing its water content and concentration of nonstructural carbohydrates. High summer temperatures increased water stress and as a consequence led to reduction in crown density. Our data indicated that the needle shedding may be also related to root system damage due to low soil temperatures. Marginally significant differences among populations in needle shedding may indicate a weak genetic control over premature needle-fall among European Scots pine populations.
The different defence strategies of trees against herbivores are very often connected with succession status, leaf life span and the level of secondary metabolites. We examined the effect of simulated leaf grazing on the differences in the leaf life span and defence chemistry of two pioneer tree species that belongs to the same family (Betulaceae), black alder (Alnus glutinosa (L.) Gaertn.) and European white birch (Betula pendula Roth.). At the beginning of the growing season, mature leaves were perforated using a paper punch. The holes removed about 10% of the leaf surface. Each species was represented by six trees – one branch was chosen for perforation and one branch as a control. All leaves were counted every week until their abscission. Additional damages caused by grazing insects were also noted. Undamaged birch leaves were held much longer than those of alder. The average difference in half leaf life span between control and perforated leaves was 28 days in birch and 6 days in alder. The control unperforated alder leaves were significantly (P <0.05) more often grazed by insects than those that were perforated. Leaf perforation in alder increase phenolic concentrations in the new, young leaves. In birch we did not observe these changes. The comparison of alder and birch indicate that the species with similar successional status can have different strategies of leaf defence. The birch leaves were characterized by a longer leaf life span, constitutive defence, a lack of induced defence accumulation of phenolics and earlier shedding of damaged leaves in comparison to the control. The black alder foliage had a shorter leaf life span, induced defence reaction (produced more phenolics after perforation), and only slightly earlier shedding of damaged leaves than the control.
Effects of elevated temperature and soil pollution with fluorine on host-pathogen relations were studied in seedlings of the pedunculate oak (Quercus robur L.) inoculated with oak powdery mildew (Microsphaera alphitoides Griff. et Maubl.) and control seedlings. The plants were grown for 1month in elevated temperature (on average by 1.6°C) and soil pollution with sodium fluoride (330 ppm F). The above factors did not have any significant effect on nitrogen content of leaves or on concentrations of metabolites favourable to growth and development of the fungal pathogen (total non-structural carbohydrates, including soluble carbohydrates and starch) and those unfavourable to fungi (soluble phenols, condensed tannins and lignins). The elevated temperature and fluorine pollution did not affect the leaf infection rate. However, a significant temperature × pollution interaction was observed in inoculated seedlings. At the elevated temperature, fluorine caused a less severe infection by powdery mildew. This could be due to a direct toxic effect of fluorine on the pathogen or by an indirect influence, resulting from changes in levels of other metabolites, which were not analysed in this study. The inoculation of oak seedlings with powdery mildew caused a decline in the carbohydrate content of leaves but did not have any significant effect on levels of other analysed metabolites. However, it significantly affected the distribution of phenols and lignins in oak leaves. Those compounds accumulated within necrotic lesions and in adjacent cells. Our results do not enable drawing definite conclusions on effects of a slight rise in temperature and a relatively low level of fluorine pollution of the soil on relations between the pedunculate oak and oak powdery mildew. Lower values of the leaf infection rate in seedlings growing in elevated temperature and fluorine pollution suggest that in warmer years a lower level of infection by M. alphitoides may be expected in areas affected by fluorine pollution.
One-year-old seedlings of silver birch (Betula pendula Roth.) were grown in pots filled with a soil substrate that originated from an area polluted by a phosphate fertiliser factory and characterised by a high soil Al level and low Ca/Al ratio or with a substrate from an area regarded as free from toxic pollution. In addition the effect of fertilisation with a mixture of nutrients was evaluated. Birch seedlings grew slowest in the unfertilised polluted substrate. In the unfertilised polluted substrate seedlings were characterised by high biomass allocation to roots (60% vs. 30 to 40% in control or fertilised substrate), lower diversity of ectomycorrhizae and the lowest rate of root and substrate microbial respiration. Roots of seedlings grown in the polluted soil were characterised by a significantly higher level of phenolic compounds. Fertilisation of plants grown in the polluted soil accelerated their growth, and lowered RWR (g root g-1 plant) and increased biomass allocated to foliage. Our results indicate that elimination of air pollution does not decrease the toxic effect of a polluted soil. Fertilisation may improve the condition of seedlings growing in polluted soil, however it was not able to eliminate entirely the adverse effect of soil pollution.
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