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1

Alteration of Cryptosporidium parvum [Apicomplexa: Eucoccidiorida] oocyst antigens following bleach treatment

100%
Liao S. F., Du C., Yang S., Healey M. C.
Acta Protozoologica
|
2001
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tom 40
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nr 4
2

Proteomic identification of lipid-bodies-associated proteins in maize seeds

76%
Du C., Liu A., Niu L., Cao D., Liu H., Wu X., Wang W.
Acta Physiologiae Plantarum
|
2019
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tom 41
|
nr 05
Seeds store lipids in the form of lipid bodies (LBs) for germination and early seedling growth. LBs can be easily isolated by the established floating-extraction method from oleaginous seeds containing a large quantity of LBs. Compared to oleaginous seeds, maize and other cereal seeds contain a small quantity of LBs, so it is difficult to isolate a sufficient quantity of LBs from their embryos for 2DE-based proteomic analysis. At present, only a limited number of LBs-associated proteins in maize embryos have been identified. We here reported a modified floating-extraction method using polyvinylidene difluoride disc to collect floating LBs from maize embryo extracts. The LBs-associated proteins were resolved with two-dimensional electrophoresis and identified with mass spectrometry. As a result, several well-known LBs proteins were identified in the purified LBs fraction, such as oleosin, caleosin, and steroleosin. We also identified another two LBs proteins, corticosteroid 11-β-dehydrogenase 1 and 11-β-hydroxysteroid dehydrogenase-like 5. In particular, steroleosin, corticosteroid 11-β-dehydrogenase 1, 11-β-hydroxysteroid dehydrogenase-like, and hydroxyproline-rich glycoprotein were found as the most abundant protein components in maize LBs. The data set of maize LBs subproteome would provide insights into functional research of LBs-associated proteins during seed development and germination. Additionally, the protocol developed here is expected to be applicable for isolating LBs in other seeds or tissues containing a low quantity of LBs.
3

Interaction of boron and aluminum on the physiological characteristics of rape (Brassica napus L.) seedlings

76%
Yan L., Riaz M., Wu X., Wang Y., Du C., Jiang C.
Acta Physiologiae Plantarum
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2018
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tom 40
|
nr 02
It has been reported that aluminum (Al) toxicity is a major limiting factor for plant growth and production on acidic soils. Boron (B) is indispensable micronutrient for normal growth of higher plants, and its addition could alleviate Al toxicity. The rape seedlings were grown under three B (0.25, 25 and 500 μM) and two Al concentrations [0 (−Al) and 100 μM (+Al) as AlCl₃·6H₂O]. The results indicated that Al stress severely hampered root elongation and root activity at 0.25 μM B while the normal (25 μM) and excess (500 μM) B improved the biomass of rape seedlings under Al exposure. Additionally, normal and excess B treatment reduced accumulation of Al in the roots and leaves under Al toxicity, which was also confirmed by hematoxylin with light staining. This indicates that both normal and excess B could alleviate Al toxicity. Furthermore, it also decreased the contents of malondialdehyde and soluble protein under Al toxicity. Likewise, superoxide dismutase activity (SOD) improved by 97.82 and 131.96% in the roots, and 168 and 119.88% in the leaves at 25 and 500 µM B, respectively, while the peroxidase and catalase activities dropped as a result of Al stress. The study results demonstrated that appropriate B application is necessary to avoid the harmful consequences of Al toxicity in rape seedlings.
4
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Molecular characterization and expression patterns of ghrelin in the reindeer (Rangifer tarandus)

64%
Zhang M., Zhu X., Xu X., Jin X., Bao H., Dugger S., Du C., Cao G., Yang Y.
Polish Journal of Veterinary Sciences
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2018
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tom 21
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nr 1
5

Morphological, physiological and genetic analyses of an upward-curling leaf mutant in Brassica napus L.

64%
Faisal S., Guo Y., Du C., Zhang D., Lv J., Channa S. A., Qu G., Hu S.
Acta Physiologiae Plantarum
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2020
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tom 42
|
nr 04
6

A review of gene sequencing in infertility

64%
Zhao J., Sha R.-N., Liu F., Du C., Dai B., Zhang Y.-B., Tian R.-G., He J.-F., Chen X.-J.
Folia Biologica
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2020
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tom 68
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nr 3
7

Vertical-flow constructed wetlands applied in a recirculating aquaculture system for channel catfish culture: effects on water quality and zooplankton

64%
Zhang S.-Y., Zhou Q.-H., Xu D., He F., Cheng S.-P., Liang W., Du C., Wu Z.-B.
Polish Journal of Environmental Studies
|
2010
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tom 19
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nr 5
In recent decades, considerable attention has been paid to the serious water pollution caused by the fastgrowing aquaculture industry. On the other side, water quality determines to a great extent the success or failure of an aquaculture operation. So highlighted is the need for sustainable development of aquaculture. In the present work, we established a recirculating aquaculture system (RAS) by vertical-flow constructed wetlands (CWs) for channel catfish (Ictalurus punctatus) culture, and assessed its effects on water quality improvement. The results indicated that the CWs applied in the RAS showed relatively higher removal efficiency for particulate matter (more than 55%) and lower, uneven removal efficiency for nutrients and organic matter (from -34.1% to 48.7%). Paired t-tests showed that only parameters of NH4 +-N, TN, COD, BOD5, TSS, and Chl-a were significantly (p<0.05) lowered after wetland treatment. Despite this, nutrients (but NH4 +-N, NO2--N, and NO3--N), organic matter, and suspended solids (including plankton) in the recirculating ponds were significantly lowered compared to the control, indicating a decline in trophic status. Multivariate analyses revealed strong relationships between zooplankton community structure and the measured environment in the culture ponds. Cyanobacterial blooms that occurred heavily in the control were strongly restrained in the recirculating ponds. This led to water quality that was suitable for fish culture. Hereby, conclusions could be reached that the recirlulating treatment by the CWs achieved its aim of sustaining or extending water quality improvement in the RAS.
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