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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Detectibility of antibodies against Borrelia burgdorferi among forest workers in North-Eastern Poland

100%
Pancewicz S. A., Kondrusik M., Zajkowska J. M., Snarska-Furla I., Swierzbinska R., Hermanowska-Szpakowicz T.
Wiadomości Parazytologiczne
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1998
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tom 44
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nr 3
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Prevalence of DNA and antibodies to Borrelia burgdorferi sensu lato in dogs suspected of borreliosis

84%
Skotarczak B., Wodecka B., Rymaszewska A., Sawczuk M., Maciejewska A., Adamska M., Hermanowska-Szpakowicz T., Swierzbinska R.
Annals of Agricultural and Environmental Medicine
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2005
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tom 12
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nr 2
199-205
The aim of the paper was an attempt to correlate clinical signs with the presence of DNA of Borrelia burgdorferi (sensu lato) s.l. and the antibodies against B. burgdorferi s.l. in the blood of dogs. Among the animals studied there were 62 dogs delivered to the Veterinary Clinic in Szczecin and 30 from the Municipal Animal Shelter in Szczecin with varied clinical signs of borreliosis. In all cases the owners admitted frequent contacts of their dogs with ticks, both in the past, as well as shortly before the onset of sickness. We used two methods: PCR for detecting DNA of B. burgdorferi s.l. and ELISA test for detecting antibodies against the spirochete. Lameness, the principal symptom of canine borreliosis was the most frequent symptom of the group of 31 PCR-positive animals. The other most common symptoms in PCR-positive dogs were fever, swelling of joints and loss of body weight. DNA of B. burgdorferi s.l. was most frequently detected in the blood of dogs of the group 2-5 years old (13/54.1%). ELISA tests specific for IgG antibodies were positive in 37 of 92 sera (40.2%) taken from examined dogs. Lameness was observed in 15 of 37 IgG-seropositive dogs and in 25 of 55 seronegative animals. In 54% of dogs with the antibodies, swelling of instep- and wrist joints was observed compared to only 24.4% in seronegative dogs. An attempt to correlate the PCR results with the results of tests detecting antibodies against B. burgdorferi s.l. revealed that fewer than half (45.1%) of the dogs with presence of DNA of the spirochete, developed an immune response. Therefore the transfer of B. burgdorferi s.l. form, the primary lesion to the target tissues, is possible in dogs which did not develop immune response or develop an insufficient response. Among 92 borreliosis-suspected dogs 54 (over 58%) were diagnosed positively using laboratory methods. In most cases there was a correlation between clinical symptoms of borreliosis and presence of DNA B. burgdorferi, thus PCR may contribute to improving to a large extent diagnostic of canine Lyme disease.
3
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Synthesis of Th17 cytokines in the culture of peripheral blood mononuclear cells stimulated with Borrelia burgdorferi sensu lato

84%
Grygorczuk S., Swierzbinska R., Moniuszko A., Kondrusik M., Zajkowska J., Czupryna P., Dunaj J., Pancewicz S.
Annals of Agricultural and Environmental Medicine
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2016
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tom 23
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nr 2
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Borrelia burgdorferi genospecies detection by RLB hybridization in Ixodes ricinus ticks from different sites of North-Eastern Poland

84%
Dunaj J., Zakowska J., Kondrusik M., Gern L., Rais O., Moniuszko A., Pancewicz S., Swierzbinska R.
Annals of Agricultural and Environmental Medicine
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2014
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tom 21
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nr 2
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Molecular and serological diagnosis of Borrellia burgdorferi infection among patients with diagnosed erythema migrans

84%
Kondrusik M., Grygorczuk S., Skotarczak B., Wodecka B., Rymaszewska A., Pancewicz S., Zajkowska J., Swierzbinska R., Hermanowska-Szpakowicz T.
Annals of Agricultural and Environmental Medicine
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2007
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tom 14
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nr 2
The aim of the study was to assess the frequency of Borrelia burgdorferi DNA detection in the blood and urine of patients diagnosed with erythema migrans, and compare the results of PCR-based methods with ELISA methodology. The latter was used to detect serum antibodies against Borrelia burgdorferi of the IgM and IgG classes, before and after antibiotic therapy. The study included 86 patients hospitalized in the Department of Infectious Diseases and Neuroinfections in the Medical Academy in Białystok, diagnosed with the erythema migrans phase of Lyme borreliosis. Examinations were carried out twice: the fi rst at the moment of diagnosis (Trial 1), the second after 4 weeks of antibiotic therapy. The study showed that antibiotic therapy in the early phase of borreliosis does not decrease the sensitivity of PCR and that after 4 weeks of therapy (Trial 2), spirochete DNA is still detectable in most patients (45/86). There was no correlation between detectability of spirochete DNA and the presence of antibodies against B. burgdorferi s.l. (assessed by ELISA) during the course of erythema migrans. The largest percentage of positive results in the detection of B. burgdorferi s.l. DNA was observed in patients who simultaneously possessed IgM and IgG antibodies against B. burgdorferi, while the lowest percentage of PCR positive results was among patients with only IgM antibodies.
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