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High intracellular Zn2plus ions modulate the VHR, ZAP-70 and ERK activities of LNCaP prostate cancer cells

100%

Wong P. F., Abubakar S.

Cellular and Molecular Biology Letters

2008

tom 13

nr 3

375-390

Malignant prostate tissues have markedly reduced zinc (Zn2+) contents in comparison to non-malignant tissues. In this study, we restored a high intracellular Zn2+ level to LNCaP prostate cancer cells by culturing the cells in a growth medium supplemented with a supraphysiological concentration of Zn2+ (10 μg/ml) over 5 weeks. The intracellular Zn2+ level increased in the Zn2+-treated cells, and there was a marked increase in the presence of zincosomes, a Zn2+-specific intracellular organelle. The proliferation rate of the Zn2+-treated cells was markedly reduced. There was also a significant increase (36.6% ± 6.4%) in the total tyrosine phosphorylated proteins. Vaccinia H1-related (VHR) phosphatase, zeta chain-associated protein-70 (ZAP-70) kinase and phosphorylated extracellular signal-regulated protein kinase 1 and 2 (p-ERK 1 and 2) were also present in higher abundance. Treatment with TPEN, which chelates Zn2+, reduced the abundance of VHR phosphatase and ZAP-70 kinase, but increased the abundance of p-ERK 1. However, the TPEN treatment restored the Zn2+-treated LNCaP cell proliferation to a rate comparable to that of the non Zn2+-treated cells. These results highlight the importance of a high intracellular Zn2+ content and the VHR/ZAP-70-associated pathways in the modulation of LNCaP prostate cancer cell growth.

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Plant: a necessity of life

80%

Abubakar B. U., Abubakar S., Alaku C., Nnadi O.

International Letters of Natural Sciences

2014

tom 15

nr 2

Plant is one of the major forms of life on earth. It can produce its own food but cannot move about. Plants have well organized structures. They are beautiful and they determine local climates and are used as live fences. They phytoremediate and cool our environment. They provide us with oxygen, medicines, fuel, timber, recreation, industrial products; preservatives, pesticides in addition to all the three necessities of life: food, clothing and shelter. They purify the air and water bodies. It is therefore impossible to talk about life without plants because of their importance to the balance of nature. However, people have altered and often degraded their environment, though it has been taking place so gradually that its effects have not been drastically felt. Despite the enormous benefits of plants to humanity, they are disappearing at alarming rates. The reasons for this loss are many and include many anthropogenic activities. It is therefore quite imperative to ensure their effective conservation. This paper summarizes available information about the direct and the indirect benefits of plants and suggests some important measures to ensure their posterity. I hope, by bringing this information together, people will appreciate our plants and be frugal in using them.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 International Letters of Natural Sciences

2 Abubakar S.

1 Abubakar B. U.

1 Alaku C.

1 Nnadi O.

1 Wong P. F.

1 2014

1 2008

Wyniki wyszukiwania

High intracellular Zn2plus ions modulate the VHR, ZAP-70 and ERK activities of LNCaP prostate cancer cells

Plant: a necessity of life