Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Modulation of inflammatory response by pentoxifylline is independent of heme oxygenase-1 pathway

100%

Taha H., Grochot-Przeczek A., Was H., Kotlinowski J., Kozakowska M., Marek A., Skrzypek K., Lackowska B., Balcerczyk A., Mustafa S., Dulak J., Jozkowicz A.

Journal of Physiology and Pharmacology

2009

tom 60

nr 2

3-12

Objective: It was reported that some effects of pentoxifylline (PTX) are mediated by heme oxygenase-1 (HO-1) induction. We investigated the role of HO-1 in anti-inflammatory activity of PTX. Methods: Experiments were performed in human and murine monocytes and endothelial cells and in HO-1 deficient mice. Results: PTX dose-dependently decreased expression of HO-1 in cell lines studied. As expected, PTX reduced also production of TNF. This effect was independent of HO-1 activity, as demonstrated in cells treated with HO-1 activators and inhibitors or in cells overexpressing HO-1. Moreover, inhibition of TNF was the same in human endothelial cells of different HO-1 genotypes, showing that PTX is similarly efficient in carriers of more and less active HO-1 promoter variants. In mice, PTX did not influence HO-1 expression, as measured in liver, kidney, spleen, heart, and skin. Accordingly, the response of PTX treated animals to LPS was the same in wild type and HO-1 deficient mice. PTX to a similar extent increased influx of leukocyte into peritoneal cavity, decreased production of TNF and reduced expression of VCAM-1 in vascular intima. Conclusion: PTX inhibits production of TNF and may decrease inflammatory reaction both in vitro and in vivo, but these effects are independent of HO-1.

Ograniczanie wyników

1 Journal of Physiology and Pharmacology

1 Balcerczyk A.

1 Dulak J.

1 Grochot-Przeczek A.

1 Jozkowicz A.

1 Kotlinowski J.

1 Kozakowska M.

1 Lackowska B.

1 Marek A.

1 Mustafa S.

1 Skrzypek K.

1 Taha H.

1 Was H.

1 2009

Wyniki wyszukiwania

Modulation of inflammatory response by pentoxifylline is independent of heme oxygenase-1 pathway