The division and growth of most types of plant cells kept under in vitro culture requires that the medium has an outside source of auxin. Phytohormones stimulate morphology and are essential ingredients of the medium used in the in vitro culture. This article presents an experiment whose aim was to evaluate the impact of indole-3-butyric acid (IBA) on the regeneration capability of explants of six doubled haploid lines of rapeseed (Brassica napus ssp. oleifera) kept under in vitro conditions. The cotyledons and hypocotyls were incubated on an MS medium with the addition of 10 mg/l indole- 3-butyric acid (IBA).The regeneration assessment was made after 28 days of culture. For all the tested DH lines we observed both substantial genotype differentiation concerning shoot regeneration effectiveness and considerable impact on shoot regeneration of adding the regulator to the medium. The average shoot regeneration effectiveness of the explants incubated on the medium containing auxin was 19.3% with the range of variability falling in between 6.7% and 42.2%.