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Increased postharvest peroxidase activity in selenium enriched mushrooms Agaricus bisporus

100%

Debski B., Milner J. A., Beelman R. B.

Żywienie Człowieka i Metabolizm

2003

tom 30

nr 3-4

Edible mushrooms (Agaricus bisporus) were enriched with an irrigation solution containing or not fortified with selenium in form of sodium selenite. Se in control mushrooms was 1.04 mcg/g D.M. and in mushrooms subjected irrigations with water solution of Se this increased to 15 mg of Se/dm) after the first flush and rose to with subsequent flushes to 30.14 mcg/g D.M. Peroxidase activity offresh mushrooms was reduced in Se-enriched Agaricus bisporus. Postharvest storage Peroxidase activity increase for 6 days and then a depression in activity .occutred. A positive correlation between peroxidase activity and fruit body mass of mushrooms was observed. Following fractionation on Sephadex G-100 column 4 peaks of peroxidases activity was observed in water extracts of mushrooms regardless of Se treatment. Molecular mass of these protein fractions were estimated by molecular mass markers as 82, 57, 45 and 22 kDa. Substantial part of peroxidase activity appears to originated from Se-dependent glutathione peroxidase.

Ograniczanie wyników

1 Żywienie Człowieka i Metabolizm

1 Beelman R. B.

1 Debski B.

1 Milner J. A.

1 2003

Wyniki wyszukiwania

Increased postharvest peroxidase activity in selenium enriched mushrooms Agaricus bisporus