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This paper deals with the bio-synthesis of silver nano-particles using a soil fungi. In this work, FTIR examinations indicated that the encountered soil particles actually contained Silver particles, while XRD analysis confirmed the silver was in the nano-crystalline phase. Moreover, the hurried decline of silver (Ag+) ions monitored using UV-visible spectrophotometer, demonstrated that these silver nano-particles formed within 19 minutes. In addition, transmission electron microscopy (TEM) revealed that the synthesized silver nano-particles come in a size ranging between 16-57 nm and have a variety of shapes, including round, rod and uneven. The present study thus concludes that silver nano-particles were actually produced and that these have admirable antibacterial activity. The current study also indicates that increasing concentration increases the rate of reduction and decreases the particle size.
In the present study Limonia acidissima Groff. ethanolic leaves extract was used for the detection of its antidermatophytic assay. It results broad spectrum of antifungals and antibacterial. Where the antimycotic activity against Trichophyton rubrum, Trichophyton tonsurans, Trichophyton mentagrophytes, Microsporium gypseum, Candida albicans and four Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa were more pronounced. The effective antidermatophytic activity observed against M. gypseum, T. tonsurans, E. coli and B. subtilis. Minimum Inhibitory Concentration of all the test microbes was determined using broth dilution technique. MFC, MBC also standardized. Preliminary Phytochemical tests were carried for the revealing basics antimicrobial responsible molecules of secondary metabolites.
Antimycotic activity of Petroleum ether and 98% methanolic young leaf soxhlet extract of Solanum nigrum (Solanaceae) was evaluated against dermatophytic fungi namely, Trichophyton rubrum, Trichophyton tonsurans, Trichophyton mentagrophytes, Microsporium gypseum, Candida albicans, and bacteria like, Staphylococcus aureus, Psudomonas aeruginosa, Bacillus subtilis, Escherichia coli. The maximum activity was observed in interpolar methanolic extract when compared to low polar petroleum ether extract. The minimum inhibitory concentration, minimum fungicidal concentration and minimum bactericidal concentration were determined against all the test strains. This study provides a basis for the isolation and purification of anti-dermatophytic compounds from the young leaves of S. nigrum.
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