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Easy PCR screening of Pichia pastoris transformants

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Arora D., Chauhan A., Khanna N.

Cellular and Molecular Biology Letters

1998

tom 03

nr 1

A simple protocol for easy PCR screening of P. pastoris transformants is described. In short, the P. pastoris cells are lysed with very small amount of the enzyme Zymolase and the crude cell lysate is directly used in PCR. This protocol needs no tube transfer steps and also obliviates the requirement of freezing the samples at -80°C before PCR screening. Because of a single step screenig, both overall and actual hands on time are considerably reduced.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Arora D.

1 Chauhan A.

1 Khanna N.

1 1998

Wyniki wyszukiwania

Easy PCR screening of Pichia pastoris transformants