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Considering the fact that certain phenotypic properties found in M. pachydermatis strains can indirectly indicate their pathogenicity, we have evaluated the morphological traits of the fungus (cell size and shape as well as colony type), expression of extracellular hydrolases (API-ZYM) and the activity of catalase, urease, casein, beta-glucosidase, phospholipase and the Tween hydrolases: 20, 40, 60 and 80. Considering the source of the analyzed strains’ origin as a differentiation criterium (animals clinically healthy and with otitis externa symptoms), it was found that the isolates obtained from the pathological material were generally (80%) characterized with a smooth growth type of light cream, large or medium-sized colonies, as well as high a activity of lipolytic enzymes, such as phospholipase and hydrolase Tween 80 and valine arylamidase, cystine arylamidase and basic phosphatase. Determining a profile of M. pachydermatis phenotypic characteristics may substantially facilitate defining the pathogenicity of the strains isolated directly from the clinical material.
Cryopreservation (storage in liquid nitrogen, –196°C) is a technique that ensures safe, long−term conservation of plant species with recalcitrant seeds, vegetatively propagated species and biotechnology products such as somatic embryos, cell lines and genetically transformed material. The aim of cryostorage is to keep plant tissue in a safe way, which does not cause changes during storage at neither phenotypic, cytological and histological, biochemical, genetic nor epigenetic level. Therefore, before cryostorage will be applied for long−term preservation it should be thoroughly assess if it does not cause any injuries in plant material.
Application of the method of analysis of main components aiming at recognition of phenotypic and genotypie divergence of several spring wheat varieties is presented in the paper. The analysis of main components was performed on three -year means for varieties and their GCA effects. Six traits / GCA effects/ or seven traits /phenotypic means/ concerning the grain yield per plant and components of the former were taken into consideration. Three first main components obtained for phenotypic means explain 86% of general variance of traits. In case of GCA effects two first main components explain over 87% of the general variance. The first main components for varietal means and GCA effects of the varieties tested discriminate them mainly in accordance with the two interrelated traits: number of stalks and number of ears per plant* The second main components determine discrimination in relation to the ear length. The Mephisto variety /FRG/ shows the greatest phenotypic and genotypie distance in relation to the remaining varieties. On the whole, a conformability between the results for varietal means and GCA effects has been found. In the light of the investigations as presented above the method of main components can be regarded as useful for investigations of divergence between genotypes.
Исчисляли (в эл) маргинальный доход связанный со следующими признаками свиней: число откормленных поросят (639 зл/год.), использoвание корма (1890 зл/овс,един.), суточный привес (3,04 зл/г) и сoдержание мяса в тушах (386 зл/1%) по ценах 1986 г. Значение единицы генетического стандартного отклонения признака содержания мяса в туше было гораздо выше остальных признаков.
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