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The aim of this research was to determine the total content of zinc and its fractions in arable soils of the Province of Podlasie (województwo podlaskie) and to assess the correlation between the zinc content, its fractions and physicochemical properties of soils. The research material consisted of samples taken from arable soils in 81 points of the province, with one point chosen in the majority of the districts. Each point was located on mineral soil used as arable land, without any external source of contamination like roads or industrial plants. Samples were taken after harvest, from the arable layer, determined to lie within the depth of 0-30cm. The samples were submitted to determinations of the basic physicochemical properties of soil. The content of total zinc was determined after mineralization in aqua regia, by the flame atomic absorption spectrometry method. The content of zinc fractions was determined by the BCR method. There were four fractions determined: exchangeable, bound with oxides and hydroxides, bound with organic matter and residual zinc. The physicochemical properties of soils were highly differentiated except the grain-size composition. The content of total zinc was typical of arable uncontaminated soils and fluctuated in a wide range. The content and shares of zinc fractions in total zinc were as follows: for very light and light soil: organic>bound with oxides and hydroxides>exchangeable>residual; for medium-heavy soil: organic>exchangeable>bound with oxides and hydroxides>residual. Distribution of zinc in particular fractions was dependent to some degree on pH, content of organic matter and grain-size composition.
The content of trace elements (Cu, Cr, Ni, Pb, and Zn) in hair and skin of silver fox (Vulpes vulpes) and arctic fox (Alopex lagopus) were determined. The content of elements were determined using inductively coupled plasma atomic emission spectrometry (ICP-OES). Samples of animal hair and skin were collected from two farms located in mid-western Poland: farm A, situated in a rural area and farm B, situated in a typically urbanized and industrial region. The influence of farm location on the concentration of Cr, Cu, and Zn in fur coat, and Cr and Ni in skin was observed.
Progressing urbanization results in the potential increase of pollution sources such as wastes, industrial or municipal sewage, which may contain hazardous inorganic pollutants such as e.g. metal ions and their compounds. Urbanization has a negative effect also on soil, as a consequence of an increasing share of impermeable surfaces such as pavements, parking lots, housing developments and public buildings. The area of a hardened (impermeable) surface is exposed to intensive surface runoff during rain and as a result pollutants are transported through the storm drainage system directly to surface waters. The aim of this study was to determine the effect of metals contained in rainwater discharged from subcatchments of various land use types on the quality of surface waters (the Cybina River and the Antoninek reservoir) in the city of Poznań, as well as investigate whether metal contents in rainwater effluents depend on the relief and character of the immediate catchment. Investigations were conducted in 2009 along the lower section of the Cybina River. Water samples from the river and reservoir were collected each time when collecting rain water samples. Water and sewage samples were collected 15 times within a year. Analyses were conducted on 5 selected catchments (with different land uses) drained by storm water drainage system discharged to the Cybina River and the Antoninek reservoir. Water was collected from 13 sampling points (5 of which were sewer outfalls and 8 were situated on the river or the reservoir above and below the sewage discharge). Higher concentrations of all tested elements were recorded in rainwater in comparison to the water samples coming from the river or the reservoir. Canonical Variate Analysis (CVA) model presenting differences between water concentrations of Cu, Zn, Cd, Pb and Fe and environmental variables (rainfall intensity measured in a four-point scale, location as divided into industrial areas multi- and single-family housing as well as location of sewage discharge to the river on the reservoir) showed elevated concentrations of these elements especially in water collected directly from the storm water sewer. Elevated metal concentrations in storm system effluents did not have a significant effect on the content of these pollutants in the river or the reservoir. It could have been caused by the intensive immobilization of elemental ions in bottom deposits followed by the metal uptake by aquatic organisms, mainly plants.
In Poland 11% of soils feature elevated zinc content or slight zinc contamination. This investigation aimed at estimation of the effect of progressive acidification of slightly zinccontaminated soils on zinc content in plants and translocation of this metal downwards the soil profile. The study involved a two-year lysimetric experiment on lessive soil. The amount of zinc indicating slight soil contamination was introduced into 0.2 m of topsoil, which was subjected to progressive acidification with sulfuric acid solution in the course of the experiment. Zinc content proved to considerably increase in plants (barley straw and maize) only under strong acidification. Soil reaction did not significantly influence the zinc content in soil, both total and assayed in HCl zinc forms, while a considerable increase in easily soluble zinc forms (in CaCl2 solution) occurred on strongly acidified soils. Considering the whole research period, increasing soil acidification did not result in any alterations involving zinc content in Bbr and C horizons of soil profiles (below 30 cm).
The impact of mineral fertilization with or without multi-component fertilizers on the content of microelements in soil and spring triticale grains was investigated in field trials, in 2009-2011. The experiment was carried out on 8 fertilizing treatments with three replications, which included two varieties of spring triticale: Andrus and Milewo. The content of available zinc and manganese was higher on plots cropped with the cultivar Andrus and nitrogen fertilization with urea or with urea and ammonium nitrate. It was also found out that the content of available manganese, zinc and iron in the analyzed soils was within the natural average range. A higher content of manganese and zinc in grains was detected after the application of multi-component fertilizers. Nitrogen fertilization at a dose of 120 kg.ha-1 together with Azofoska and Ekolist resulted in an increase in the iron content in cv. Andrus. The regression analysis between the content of the analyzed microelements in soil and in triticale grains revealed a significant increase in the iron, manganese and zinc content in grains together with an increase in the content of these elements in soil under cv. Milewo. With respect to the zinc content in soil and in grain from this variety, the coefficient of determination was the closest to the coefficient of a linear correlation (R2 = 0.9105). It was shown that an increase in the content of microelements in soil was not always accompanied by an increase in the content of these elements in spring triticale grains.
The aim of the study was to analyze the total content of micronutrients in the soils in some habitats in the area of the Jezierzyca River Valley Landscape Park. Experimental sites were chosen with regard to different habitats (humid mixed coniferous forest, fresh mixed coniferous forest, humid mixed forest, fresh mixed forest). There are arenosoils, rusty and podzol soils and gley soils. The soils were developed from the river alluvial sands and fluvial sands and prove the acidic reaction. Results showed that the total content of Mn, Zn and Cu is highest in the organic levels and decreases with the depth in the analyzed soils. Only in the podzols soils the total content of Mn decreases in the eluvial horizon and increases in the iluvial horizon. The conducted investigations indicate no pollution of the soils with the micronutrients. The exception was a higher content of Cu in the superficial level of soils in humid and fresh mixed coniferous forest.
In a vegetation experiment, the effect of composts made from unsorted municipal solid waste and urban green waste on the content of zinc in plants has been analyzed. The municipal waste composts matured in heaps for 1, 3 and 6 months. They were applied in three rates: 10, 20 and 30 g kg–1 of soil. The compost made of urban green waste aged in a pile for 6 months and was added to soil in the amount of 10 g kg–1 of soil. It has been determined that the content of zinc in plants was varied and depended on the type and rates of compost used as well as the species of crops. Application of higher rates of composts did not invariably result in an increased concentration of zinc in plant tissues; on the contrary, in some cases the level of zinc determined in fertilized plants was lower than in the control. Much more elevated concentrations of zinc were found after the application of fresh compost made of municipal waste (maturing for one month in a heap). Fertilization of maize and barley with urban green waste compost produced a more favourable result than the application of analogous rates of municpal solid waste compost, as the plants contained less zinc. With respect to the other test plants, such as sunflower, mustard and phacelia, the application of composted green matter caused a considerable increase in the content of zinc, as compared with the treatments fertilized with composted municipal waste, at the same fertilization rates.
The effect of different ripening stages of durian Durio zibethinus fruit on zinc content in liver of rats loaded with cholesterol. Durian Durio zibethinus is an exotic, climacteric fruit, rich in dietary fiber, polyphenols, saccharides, vitamins and minerals. Postharvest changes in durian fruit influence its physical and chemical quality, which determined its health protective effect – antioxidant and hypocholesterolemic. Durian is a good source of zinc. The aim of this study was to investigate the zinc content in lyophilisate of durian fruit and in the diets supplemented with durian fruits at different stages of maturity. The in vivo experiment was designed to determine the influence of these diets on zinc content in the liver of rats loaded with cholesterol. The zinc content in durian fruit, in the diets, and in rats livers was examined using a flame atomic absorption spectrophotometry (Perkin-Elemer 1100B). The zinc content in durian fruits ranged from 8.1 to 12.3 mg/kg. The zinc content amounted 45.0, 44.2, 47.1, 47.3 and 48.0 mg/kg in C, ch, chDM, ch DR and chDOR diet, respectively. The content of zinc in the liver in all animal groups was within the normal limits and amounted from 25.0 to 33.0 mg/kg fresh weight. Diet supplemented with durian fruits at different stages of ripening increases accumulation of zinc in the liver of rats loaded with cholesterol. Durian fruits, especially ripe ones, could be used as a natural supplementation of zinc in the diet and might be helpful in prevention of diseases or disorders related to zinc deficiency.
The article presents an overview of the results of a study concerning the zinc and lead content in urban soils of Kielce with regard to different land use. 61 topsoil samples were collected in the industrial areas (I), urban green areas (Z), urban allotment gardens (O), as well as parks and forests (P). The maximum values for zinc and lead were observed in the industrial zone, i.e. 181.3 mg kg-1 and 112.6 mg kg-1, respectively; whereas the average content in the soils of Kielce oscillates at the level of 134.2 mg kg-1 for zinc and 92.85 mg kg-1 for lead. Geochemical indices, such as the geoaccumulation index (Igeo), contamination factor (CF) and accumulation index (AI) indicated that areas located in close proximity to the main roads of the city, especially in the city centre and the former industrial estate called Białogon, are characterised by the highest concentration of the analysed heavy metals. Generally, the enrichment of soils in zinc and lead decreases further from the city centre. In most cases, the content of the analysed heavy metals in surface layers of urban soils considerably exceeded values considered as geochemical background and valid legal norms of the Ordinance of the Minister of Environment on soil quality standards and land quality standards (Journal of Laws of 2002, No. 165, item 1359). The exceeded values of the analysed metals are caused mainly by deposition of contamination from car exhaust emissions and broadly defined industry.
Diets supplemented with Mytilus galloprovincialis from polluted and non-polluted waters and their influence on zinc content in liver of rats loaded with cholesterol. Mussels may reflect the environmental problems that the ecosystem faces. Mytilus galloprovincialis is widely used as an indicator of water pollution in biological monitoring studies. These mussels are filter feeders and may accumulate important ecosystem pollutants what in turn may pose a potential risk to other organisms in the food chain. The aim of the study was to determine zinc content in the diets supplemented with mussels from polluted and non-polluted areas and in the livers of rats fed these diets for 30 days. The zinc content in the diets with mussels from contaminated and noncontaminated waters and in rat livers was examined using a flame atomic absorption spectrophotometry (Perkin-Elmer 1100B). The slight increase in zinc content from 73.2 ±1.2 mg/ /kg in ch diet (control with 1% of cholesterol) to 77.2 ±0.5 mg/kg in the diet with mussels from polluted site was found, but zinc content in all diet was within the normal limits reviewed by McDowell (1992) – 40 to 100 ppm. Zinc content in the diet supplemented with mussels from nonpolluted site (73.7 ±3.7 mg/kg) was similar to that found in ch diet. It can be concluded that cholesterol does not affect zinc bioaccumulation. However, prolonged consumption of mussels from contaminated sites may increase accumulation of zinc in the liver of rats.
Malignant prostate tissues have markedly reduced zinc (Zn2+) contents in comparison to non-malignant tissues. In this study, we restored a high intracellular Zn2+ level to LNCaP prostate cancer cells by culturing the cells in a growth medium supplemented with a supraphysiological concentration of Zn2+ (10 μg/ml) over 5 weeks. The intracellular Zn2+ level increased in the Zn2+-treated cells, and there was a marked increase in the presence of zincosomes, a Zn2+-specific intracellular organelle. The proliferation rate of the Zn2+-treated cells was markedly reduced. There was also a significant increase (36.6% ± 6.4%) in the total tyrosine phosphorylated proteins. Vaccinia H1-related (VHR) phosphatase, zeta chain-associated protein-70 (ZAP-70) kinase and phosphorylated extracellular signal-regulated protein kinase 1 and 2 (p-ERK 1 and 2) were also present in higher abundance. Treatment with TPEN, which chelates Zn2+, reduced the abundance of VHR phosphatase and ZAP-70 kinase, but increased the abundance of p-ERK 1. However, the TPEN treatment restored the Zn2+-treated LNCaP cell proliferation to a rate comparable to that of the non Zn2+-treated cells. These results highlight the importance of a high intracellular Zn2+ content and the VHR/ZAP-70-associated pathways in the modulation of LNCaP prostate cancer cell growth.
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The aim of this work was the assessment of the age, sex, way of nutrition, environment and health state influence on zinc content in hair of population living in the Middle Pomerania. Within years of 2005-2007 hair coming from 416 persons from a ten months old child to a 75 years old person was analyzed. All people were divided into four age groups: children (0-15 years), youth (16-25), adults (26-50) and the elderly (51-75). 3-centimeters long hair (counting from the skin) were put to chemical analyze. Then dried samples were weighed and mineralized with mixture of nitric and tetraoxochloric acides (mixed in proportion of 5:1). Zink determination was done by method of atomic absorption spectrophotometry using spectrometer ASA-3. Average zinc content in hair of researched population amounted to 167±58 7g g-1. Least of all zinc was ascertained in children hair (av. 125 7g g-1), and most of all in youth (194 7g g-1). In all age groups females had more of this metal (av. 180 7g g-1) than males (156 7g g-1). Average value of zinc for people living in a village was 147 7g g-1, and living in towns and cities 181 7g g-1. The least of zinc we have found at persons being on the meat- or milk-free diet (av. 157 7g g-1) and the most at persons that did not restrict in eating meat and milk products (197 7g g-1). The persons suffering from cardiovascular disease or hyperplasia prostate had adequately 221 and 195 7g g-1 Zn, considerably above the level in hair of without symptoms diseases persons (156 7g g-1). Smaller content of this element had persons suffering from atherosclerosis or hypertension (adequately 132 and 142 7g g-1).
The contents of cadmium, lead, zinc and copper were determined in 64 samples of produce (19 strawberries, 16 tops of parsley, 16 dills, 13 lettuces). The produce was gathered directly from home-gardens located near Opole, Wroclaw, Legnica and Jastrzebie Zdroj. The content of cadmium found was within the limits 0.0027–0.663 mg/kg. The contents exceeding the allowable value were found in three samples of dill. The determined quantity of lead ranged from 0.0047 mg/kg to 0.588 mg/kg and the exceeding of allowable contents was found in two dill samples, two lettuce samples and one parsley (greens). The level of zinc contents was between 0.716–34.76 mg/kg. In fourteen samples (3 parsley, 2 lettuces and 9 dills) the limits established before 2004 were exceeded.The contents of copper in produce was within 0.342–17.03 mg/kg. In seven samples (3 parsley and 4 dills) the allowable quantity of Cu established before 2001 was exceeded. The highest amount of cadmium and copper was determined in samples taken near Jastrzebie Zdroj, while the largest quantity of lead and zinc was found in the produce collected in Legnica. The relatively smallest quantities of metals were found in the samples collected near Opole. A comparison of collected data with those available in the literature shows that the recorded level of contamination of the tested produce with heavy metals is close to levels determined for other industrial regions of the country but higher than the world’s standards.
The aim of this study has been to determine the indirect and direct effects of farmyard manure and sewage sludge composts, produced in the north-eastern part of Poland, on the content of 1 mol HCl⋅dm-3 soluble forms of zinc and copper in soil. In 2004-2007, a field experiment was carried out at the Experimental Station in Bałcyny near Ostróda. The experiment was established on proper grey-brown podzolic soil originating from light boulder clay, which was rich in P, moderately abundant in K and low in Mg. It comprised a four-field crop rotation system (potato, spring barley, winter oilseed rape and winter wheat. The design of the experiment, set up according to the random block method, involved 8 objects (2 x 4): 1) farmyard manure, 2) compost (sewage sludge + straw), 3) dried and granulated sewage sludge, 4) composted sewage sludge. The composts and FYM were introduced to soil once (in 2004) at a rate of 10 t d.m.⋅ha-1 or 2 × 5 d.m.⋅ha-1 (under potato and winter oilseed rape). In 2004, nitrogen in the soil enriched with natural fertilizers was balanced to 150 kg⋅ha-1 according to the N-total content. In 2005, soil cropped with spring barley received only mineral fertilization, whereas winter oilseed rape received the second rate of organic fertilizers (in the series consisting of 2 × 5 d.m.⋅ha-1) and nitrogen was balanced to 120 kg⋅ha-1. In 2006, soil under winter wheat received only mineral fertilization. Prior to the establishment of the experiment, soil, manure and compost samples were taken. Having been averaged, the samples were subjected to determination of their content of Cu and Zn in 1 mol HCl dm-3. The soil, whose reaction was 5.04 in 1 mol HCl dm-3, was moderately abundant in available zinc and low in copper. After four years of the trials, the levels of available forms of copper and zinc in the soil fertilized with sewage sludge composts, compared to the soil enriched with FYM, were higher. In the first and fourth year of the experiment, the content of both elements was found to increase significantly in the objects fertilized with dry, granulated and composted sludge. Sludge composted with straw significantly raised the content of Zn and Cu in the second and third year of the experiment. However, fertilization of grey-brown podzolic soil with sewage sludge did not change its classification according to the abundance of available forms of copper and zinc.
The purpose of the study has been to determine the direct and residual effect of farmyard manure and composts made from sewage sludge on the content and uptake of copper and zinc by crops growing in a four-field crop rotation system. In 2004-2007, a field experiment was established on proper grey-brown podzolic soil, originating from light boulder clay, rich in P, moderately abundant in K and low in Mg, whose reaction was pH = 5.04. The experiment involved a four-field crop rotation cycle with the following crops: potato, spring barley, winter oilseed rape and winter wheat. The design of the experiment, set up according to the random block method, consisted of 8 object (2 x 4): 1) FYM, 2) composted sewage sludge, 3) compost (sewage sludge + straw), 4) dried and granulated sewage sludge. The composts and farmyard manure were applied once in the rotation system (under potato) at a rate of 10 t d.m. ha-1 or twice, each time 5 t d.m. ha-1 (under potato and under winter oilseed rape). In the objects fertilized with organic fertilizers and manure, nitrogen was balanced to 150 kg⋅ha-1 (under potato) and to 120 kg⋅ha-1 (under oilseed rape) depending on their total content of nitrogen. Spring barley and winter wheat received only mineral fertilizers. Before the experiment was set up, samples of soil, manure and composts had been collected for determination of Cu and Zn in 1 mol HCl dm-3 by atomic absorption spectrophotometry. The content of zinc and copper in the crops was modified by the form of sewage sludge and sewage sludge composts to a greater extent than by the way these fertilizers were applied in the crop rotation system. Soil fertilization with dried and granulated sewage sludge or composted sludge increased the content of copper and its removal with harvested potato and winter rape, but the analogous values were higher when the cereals were grown on FYM fertilized soil. The strongest influence on the content and removal of zinc was exerted by soil fertilization with dried and granulated sewage sludge. The index of copper and zinc uptake was to a greater degree conditioned by the species of a crop than by the type of fertilizers or their application method. Municipal sewage sludge and sewage sludge composts can be used as a substitute of manure in farms which do not keep farm animals.
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