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Ectomycorrhizal communities associated with Pinus sylvestris growing on dry coniferous forest habitat after the fire were studied. In order to investigate the fire effect on ectomycorrhizal fungi and changes of soil parameters in the upper (0−15 cm) soil layer three study plots were established: 1 – the control, 2 – fire zone left for artificial renewal (with the economic method of renewal) and 3 – fire zone left for natural renewal. The analysis of mycorrhizae revealed presence of eight mycorrhizal fungi on Scots pine roots and the value of Shannon−Wiener species diversity index H’ equaled to 1.76. The most abundant were mycorrhizae formed by Tomentella feruginea (31.7%) and Hebeloma crustuliniforme (23.3%). The fraction of Paxillus involutus and em>Rhizopogon sp. mycorrhizae was 16.7% and 13.0%, respectively. The lowest was the number of Thelephora terrestris mycorrhizae (only 1.7%). At the site 2, number of mycorrhizal fungi diminished to five and Shannon−Wiener species diversity index was also lower (1.37). The predominant were mycorrhizae of Cenococcum eophilum (26.7%) and P. involutus (21.7%), whilst the lowest were mycorrhizae of Suillus luteus (1.7%). Although at the third site the number of ectomycorrhizal fungi was the same as on the site 2, the dominance of Thelephora terrestris (66.7%) caused that Shannon−Wiener’s species diversity index was the lowest (1.05). Mycorrhizae of Paxillus involutus and Wilcoxina mikolae were characterised by similar abundance (13.3% and 11.7%, respectively). The lowest number of mycorrhizae was observed for Tomentella sp. (3.3%). Analysis of soil parameters showed an increase of pH on burnt sites in comparison to the control. The soil of burnt sites were also characterised by lower value of C and C/N ratio than the soil on the control treatment. The result showed that ubiquitous mycorrhizal fungi, such as T. terrestis, are able to persist on roots in changed soil environment with low content of nutritions.
Effects of post−harvest wood−debris utilization and pre−planting soil preparation in clear−cut forest on the community structure of soil fungi and bacteria and their possible biological activity towards Armillaria and Heterobasidion were studied in 1− and 10−year−old Scots pine plantations in Bierzwnik and Międzychód Forest Districts (W Poland). Post−harvest wood−debris utilization included: (i) removal from the surface, (ii) spread of the coarse or chipped wood−debris on the surface and (iii) mixing of the chipped wood debris with the soil. Pre−planting soil preparation included: (i) deep furrowing, (ii) shallow turning of the topsoil, (iii) ridging and (iv) no ground preparation. The soil−dilution method was used for detection of fungi and bacteria in soil. Morphotyping was used for identification of fungi. Phenotypic traits and biochemical properties were used for identification of bacteria. Molecular method, MID−66 or BIOLOG® systems were additionally applied for identification of the most common bacteria. Removal of post−harvest wood−debris from the surface of the clear−cut land and shallow turning of the topsoil or ridging before planting increased abundance of fungi in soil of 1−year−old Scots pine plantation. Deep furrowing resulted in increased abundance of fungi and no ground preparation in increased abundance of bacteria in soil of 10−year−old Scots pine plantation. Increased abundance of fungi and bacteria was associated with increased abundance of taxa considered as antagonistic to Armillaria and Heterobasidion. Removal of the post−harvest wood debris and moderate or no mechanical intervention into the soil habitat on the clear−cut site before planting of Scots pine seedlings seems to create the habitat, which may be beneficial for the growth of young trees.
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