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The aim of this study was to assess the occurrence of Trichomonas tenax in individuals with periodontal disease and also to check the correlation between the occurrence of protozoan and a kind of periodontal disease, bleeding index and dental plaque index. The study involved 77 individuals aged 22-77 years (47 women and 30 men). Culture revealed flagellate Trichomonas tenax. The incidence of trichomonal infection was 32%. In spite of using two different media no Entamoeha gingivalis was found. There was no significant dependence between the frequency of occurrence of T. tenax and age, sex or smoking. There was a correlation between the occurrence of T. tenax and the amount of dental plaque (correlation index=0.3; p=0.06) and there was also a statistical dependence between the occurrence of T. tenax and a kind of periodontal disease (correlation index=0.3; p=0.02).
The aim of study was the evaluation of periodontal pockets microflora in patients with advanced periodontitis. From each subject 16-20 samples were taken using paper points. Pooled sample after 60 s. mixing was serially diluted in reduced BHI. For tolal cell counts and for the isolation of black pigmented anaerobes Brucella agar supplemented with 5% sheep blood, hemin, menadione, with and without Kanamycin-Vancomycin mixture and BM agar plates were used. For isolation of A. actinomycetemcomitans TSBV agar plates were used. Cultures were incubated in anaerobic chamber at 37°C for 7 days and TSBV agar plates in an atmosphere of 95% air-5% CO2 at 37°C for 5 days. Microorganisms were identified by Gram staining, colony morphology, fluorescence in UV-light, haemagglutination of 3% sheep erythrocytes, fermentation of sugars, production of indole, urease (API 20A), specific enzymes (Rapid ID 32A). Twenty seven subjects with clinically recognized periodontitis were examined. Microorganisms important in periodontitis were isolated from periodontal pockets of almost all examined subjects. The number of bacteria obtained from the sample of one patient ranged from 1•10 4CFU/ml to 3,6 •10 6 CFU/ml. Porphyromonas gingivalis was identified in the samples taken from 17 patients, Prevotella intermedia-19, Actinobacillus actinomycetemcomitans-11, Fusobacterium nucleatum-9, Peptostretococcus spp. -22.
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