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Analysis of occurrence of virulence genes among Yersinia enterocolitica isolates belonging to different biotypes and serotypes

100%

Kot B., Piechota M., Jakubczak A.

Polish Journal of Veterinary Sciences

2010

tom 13

nr 1

13-19

The 150 Y. enterocolitica strains isolated from humans and from pigs belonged to biotypes 4 (68.7%), 1A (18.7%) and 2 (4%), or were biochemically untypeable (8.6%). Biotype 4 was comprised of Y. enterocolitica strains representing serotype 0:3, within biotype 1A the strains either belonged to serotypes 0:5 and 0:6 or were untypeable, and biotype 2 was represented by the strains of serotype 0:9. The strains which were biochemically untypeable belonged to serotypes 0:5, 0:6 and 0:3. Among the strains tested there also were those of an unidentified biotype and serotype. Nearly all the strains of biotype 1A represented genotype ystB+myfA+, and few belonged to genotype ystB+. The presence of the ystB gene in the strains of biotype 1A and only occasional occurrence of the gene in the other biotypes makes ystB a distinguishing marker of biotype 1A. The strains of genotype ystA+ail+myfA+yadA+ predominated in biotype 4 (serotype 0:3). The strains of biotype 2 (serotype 0:9) represented genotype ystA+ail+myfA+, and the plasmid yadA gene was detected in some of them. Within the group of biochemically untypeable strains ystB- and myfA-specific PCR products were mainly obtained. The genotypes determined for the tested biotypes and serotypes of Y. enterocolitica, based upon the selected genes of virulence, can be applied as distinguishing markers and indicators of the potential virulence of Y. enterocolitica strains, excluding bioserotyping.

Application of multiplex PCR for the evaluation of the occurrence of ystA, ystB, ystC, and ymoA genes in Yersinia enterocolitica strains isolated from fattening pigs

100%

Bancerz-Kisiel A., Szczerba-Turek A., Platt-samoraj A., Szweda W.

Bulletin of the Veterinary Institute in Puławy

2011

tom 55

nr 1

The purpose of the study was to evaluate the occurrence of genes directly connected with pathogenicity in Yersinia enterocolitica strains isolated from fattening pigs. Multiplex PCR, used for ystA, ystB, ystC, and ymoA gene detection, was optimised in order to determine the existence of the genes in one reaction. Material for the study consisted of 138 strains of Y. enterocolitica, which were preliminary examined by the bacteriological, sero- and biotyping methods, then bacterial DNA was isolated and multiplex PCR was performed. The presence of the products of length corresponding to the ystA and ymoA gene fragments was found in each of the examined strains. The ystB and ystC genes were not detected in any of the tested samples. The molecularly confirmed existence of Y. enterocolitica in fattening pigs indicates the carrier state and a possibility of shedding the microorganism into the environment, and shows that pigs are an important reservoir of the bacteria and a potential source of infection for humans.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Polish Journal of Veterinary Sciences

1 Bancerz-Kisiel A.

1 Jakubczak A.

1 Kot B.

1 Piechota M.

1 Platt-samoraj A.

1 Szczerba-Turek A.

1 Szweda W.

1 2011

1 2010

Wyniki wyszukiwania

Analysis of occurrence of virulence genes among Yersinia enterocolitica isolates belonging to different biotypes and serotypes

Application of multiplex PCR for the evaluation of the occurrence of ystA, ystB, ystC, and ymoA genes in Yersinia enterocolitica strains isolated from fattening pigs